(Q42883037)

English

Evidence that the lack of high catalytic activity of thiolsubtilisin towards specific substrates may be due to an inappropriately located proton-distribution system. Demonstration of highly nucleophilic character of the thiol group of thiolsubtilisi

scientific article published on March 1981

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scholarly article

1 reference

Europe PubMed Central

9 November 2017

Evidence that the lack of high catalytic activity of thiolsubtilisin towards specific substrates may be due to an inappropriately located proton-distribution system. Demonstration of highly nucleophilic character of the thiol group of thiolsubtilisi (English)

1 reference

Europe PubMed Central

9 November 2017

author name string

Brocklehurst K

1

1 reference

Europe PubMed Central

9 November 2017

Malthouse JP

2

1 reference

Europe PubMed Central

9 November 2017

language of work or name

0 references

publication date

1 March 1981

1 reference

Europe PubMed Central

9 November 2017

Biochemical Journal

1 reference

Europe PubMed Central

9 November 2017

193

1 reference

Europe PubMed Central

9 November 2017

819-823

1 reference

Europe PubMed Central

9 November 2017

3

1 reference

Europe PubMed Central

9 November 2017

Evidence for a two-state transition in papain that may have no close analogue in ficin. Differences in the disposition of cationic sites and hydrophobic binding areas in the active centres of papain and ficin

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1162672

27 May 2018

Evidence that the active centre of chymopapain A is different from the active centres of some other cysteine proteinases and that the Brønsted coefficient (beta nuc.) for the reactions of thiolate anions with 2,2'-dipyridyl disulphide may be decreas

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1162672

27 May 2018

Chromatography and activity of thiol-subtilisin.

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1162672

27 May 2018

Properties of thiol-subtilisin. The consequences of converting the active serine residue to cysteine in a serine protease

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1162672

27 May 2018

On the Reactivity of the Thiol Group of Thiolsubtilisin

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1162672

27 May 2018

Reactions of papain and of low-molecular-weight thiols with some aromatic disulphides. 2,2'-Dipyridyl disulphide as a convenient active-site titrant for papain even in the presence of other thiols

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1162672

27 May 2018

Negatively charged reactants as probes in the study of the essential mercaptide-imidazolium ion-pair of thiolenzymes

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1162672

27 May 2018

Mechanism of the reaction of papain with substrate-derived diazomethyl ketones. Implications for the difference in site specificity of halomethyl ketones for serine proteinases and cysteine proteinases and for stereoelectronic requirements in the pa

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1162672

27 May 2018

Evidence for association-activation effects in reactions of papain from studies on its reactivity towards isomeric two-protonic-state reactivity probes

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1162672

27 May 2018

The equilibrium assumption is valid for the kinetic treatment of most time-dependent protein-modification reactions

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1162672

27 May 2018

Specific covalent modification of thiols: Applications in the study of enzymes and other biomolecules

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1162672

27 May 2018

Mechanism of action of papain with a specific anilide substrate

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1162672

27 May 2018

Thioltrypsin. Chemical transformation of the active-site serine residue of Streptomyces griseus trypsin to a cysteine residue

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1162672

27 May 2018

Serine Proteases: Structure and Mechanism of Catalysis

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1162672

27 May 2018

Convergence of active center geometries

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1162672

27 May 2018

Evidence that binding to the s2-subsite of papain may be coupled with catalytically relevant structural change involving the cysteine-25-histidine-159 diad. Kinetics of the reaction of papain with a two-protonic-state reactivity probe containing a h

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1162672

27 May 2018

Comparison of the kinetic specificity of subtilisin and thiolsubtilisin toward n-alkyl p-nitrophenyl esters

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1162672

27 May 2018

Mechanism of thiol protease catalysis: detection and stabilization of a tetrahedral intermediate in papain catalysis

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1162672

27 May 2018

Characterization of the papain active centre by using two-protonic-state electrophiles as reactivity probes. Evidence for nucleophilic reactivity in the un-interrupted cysteine-25-histidine-159 interactive system

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1162672

27 May 2018

Re-examination of the charge relay system in subtilisin comparison with other serine proteases

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1162672

27 May 2018

Preparation of fully active ficin from Ficus glabrata by covalent chromatography and characterization of its active centre by using 2,2'-depyridyl disulphide as a reactivity probe

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1162672

27 May 2018

The Reactivity of Thiol-subtilisin, an Enzyme Containing a Synthetic Functional Group*

1 reference

https://pubmed.ncbi.nlm.nih.gov/6272719

12 December 2020

based on heuristic

inferred from PubMed ID database lookup

TRANSFORMATION OF L-SERINE PEPTIDES TO L-CYSTEINE PEPTIDES

1 reference

https://pubmed.ncbi.nlm.nih.gov/6272719

12 December 2020

based on heuristic

inferred from PubMed ID database lookup

Demonstration of the acyl-enzyme mechanism for the hydrolysis of peptides and anilides by chymotrypsin

1 reference

https://pubmed.ncbi.nlm.nih.gov/6272719

12 December 2020

based on heuristic

inferred from PubMed ID database lookup

The importance of the conformation of the tetrahedral intermediate for the α‐chymotrypsin‐catalyzed hydrolysis of peptide substrates

1 reference

https://pubmed.ncbi.nlm.nih.gov/6272719

12 December 2020

based on heuristic

inferred from PubMed ID database lookup

Cryoenzymology of papain: reaction mechanism with an ester substrate

1 reference

https://pubmed.ncbi.nlm.nih.gov/6272719

12 December 2020

based on heuristic

inferred from PubMed ID database lookup

Conformation of the active site of thiolsubtilisin: reaction with specific chloromethyl ketones and arylacryloylimidazoles

1 reference

https://pubmed.ncbi.nlm.nih.gov/6272719

12 December 2020

based on heuristic

inferred from PubMed ID database lookup

Neutron diffraction identifies His 57 as the catalytic base in trypsin

1 reference

https://pubmed.ncbi.nlm.nih.gov/6272719

12 December 2020

based on heuristic

inferred from PubMed ID database lookup

Identifiers

10.1042/BJ1930819

1 reference

Europe PubMed Central

9 November 2017

1 reference

Europe PubMed Central

9 November 2017

1 reference

Europe PubMed Central

9 November 2017

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0 references

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