(Q41682132)

7 October 2017

title

Specific covalent modification of thiols: applications in the study of enzymes and other biomolecules (English)

1 reference

https://www.ebi.ac.uk/europepmc/webservices/rest/search?resulttype=core&format=json&query=SRC%3AMED%20AND%20EXT_ID%3A456716

3 July 2022

Specific covalent modification of thiols: Applications in the study of enzymes and other biomolecules (English)

1 reference

https://api.crossref.org/works/10.1016/0020-711X(79)90088-0

21 May 2023

1 reference

https://www.ebi.ac.uk/europepmc/webservices/rest/search?resulttype=core&format=json&query=SRC%3AMED%20AND%20EXT_ID%3A456716

3 July 2022

1 reference

https://api.crossref.org/works/10.1016/0020-711X(79)90088-0

21 May 2023

author name string

K. Brocklehurst

1

1 reference

https://www.ebi.ac.uk/europepmc/webservices/rest/search?resulttype=core&format=json&query=SRC%3AMED%20AND%20EXT_ID%3A456716

3 July 2022

language of work or name

English

1 reference

National Center for Biotechnology Information

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/esummary.fcgi?db=pubmed&retmode=json&id=456716

3 July 2022

publication date

1 January 1979

1 reference

full work available at URL

7 October 2017

https://api.elsevier.com/content/article/PII:0020711X79900880?httpAccept=text/xml

1 reference

https://api.crossref.org/works/10.1016/0020-711X(79)90088-0

https://api.elsevier.com/content/article/PII:0020711X79900880?httpAccept=text/plain

21 May 2023

1 reference

https://api.crossref.org/works/10.1016/0020-711X(79)90088-0

International Journal of Biochemistry & Cell Biology

21 May 2023

published in

1 reference

7 October 2017

volume

10

1 reference

7 October 2017

issue

4

1 reference

7 October 2017

page(s)

259-274

1 reference

Electron spin resonance and nuclear relaxation studies on spin-labeled glutamate dehydrogenase

7 October 2017

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21 May 2023

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21 May 2023

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21 May 2023

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Properties of matrix-bound dimer and monomer derivatives of immobilized creatine kinase from rabbit skeletal muscle

21 May 2023

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Syncatalytic enzyme modification: characteristic features and differentiation from affinity labeling

21 May 2023

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Allosteric regulation of aspartate transcarbamoylase. Effect of active site ligands on the reactivity of sulfhydryl groups of the regulatory subunits

21 May 2023

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Synthesis and use of bifunctional chloromethylalkanedione derivatives of variable chain length for cross-linking thiol groups in oligomeric proteins. Specific cross-linking in glyceraldehyde 3-phosphate dehydrogenase

21 May 2023

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Effect of spin-labeled maleimide on 14S and 30S dyneins in solution and on demembranated ciliary axonemes

21 May 2023

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Arginine decarboxylase from Escherichia coli B: mechanism of dissociation from the decamer to the dimer

21 May 2023

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Fluorescence Probes for Structure

21 May 2023

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A novel reactivity of papain and a convenient active site titration in the presence of other thiols

21 May 2023

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Reactivities of the various protonic states in the reactions of papain and of L-cysteine with 2,2'- and with 4,4'- dipyridyl disulphide: evidence for nucleophilic reactivity in the un-ionized thiol group of the cysteine-25 residue of papain occasion

21 May 2023

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Reactions of papain and of low-molecular-weight thiols with some aromatic disulphides. 2,2'-Dipyridyl disulphide as a convenient active-site titrant for papain even in the presence of other thiols

21 May 2023

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Propapain and its Conversion to Papain: A New Type of Zymogen Activation Mechanism involving Intramolecular Thiol-Disulphide Interchange

21 May 2023

26

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PH-dependence of the steady-state rate of a two-step enzymic reaction

21 May 2023

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The pH-dependence of second-order rate constants of enzyme modification may provide free-reactant pKa values

21 May 2023

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Mechanism of the reaction of papain with substrate-derived diazomethyl ketones. Implications for the difference in site specificity of halomethyl ketones for serine proteinases and cysteine proteinases and for stereoelectronic requirements in the pa

21 May 2023

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The reaction of papain with Ellman's reagent (5,5'-dithiobis- (2-nitrobenzoate) dianion)

21 May 2023

30

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The mutability of stem bromelain: evidence for perturbation by structural transitions of the parameters that characterize the reaction of the essential thiol group of bromelain with 2,2'-dipyridyl disulphide

21 May 2023

31

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Covalent chromatography. Preparation of fully active papain from dried papaya latex

21 May 2023

32

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Covalent chromatography by thiol-disulfide interchange

21 May 2023

33

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Substituted Pyridines as Two-Protonic-State Reactivity Probes, Reporter-Group Delivery Vehicles and Labelling Reagents for the Study of Thiol Enzymes

21 May 2023

34

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Inactivation of glyceraldehyde-3-phosphate dehydrogenase and yeast alcohol dehydrogenase by arene oxides

21 May 2023

36

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Specificity of induced conformational changes. Case of yeast glyceraldehyde-3-phosphate dehydrogenase

21 May 2023

39

1 reference

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Preparation of bovine mercaptalbumin by means of covalent chromatography.

21 May 2023

41

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Reactions of L-ergothioneine and some other aminothiones with2,2'-and 4,4'-dipyridyl disulphides and of L-ergothioneine with iodoacetamide. 2-Mercaptoimidazoles, 2- and 4-thiopyridones, thiourea and thioacetamide as highly reactive neutral sulphur n

21 May 2023

42

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A convenient spectrophotometric assay for the determination of L-ergothioneine in blood

21 May 2023

43

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https://api.crossref.org/works/10.1016/0020-711X(79)90088-0

Immobilization of urease by thiol-disulphide interchange with concomitant purification.

21 May 2023

44

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Reversible, Covalent Immobilization of Enzymes by Thiol‐Disulphide Interchange

21 May 2023

45

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Protein thiolation and reversible protein-protein conjugation. N-Succinimidyl 3-(2-pyridyldithio)propionate, a new heterobifunctional reagent

21 May 2023

47

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Use of dimethyl suberimidate and novel periodate-cleavable bis(imido esters) to study the quaternary structure of the pyruvate dehydrogenase multienzyme complex of Escherichia coli

21 May 2023

50

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Spin-label studies of the sulfhydryl environment in bovine plasma albumin. 1. The N-F transition and acid expansion

21 May 2023

52

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Spin-label studies of the sulfhydryl environment in bovine plasma albumin. 2. The neutral transition and the A isomer

21 May 2023

53

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Affinity Chromatography of Macromolecules

21 May 2023

56

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Mercurated polynucleotides. New probes for hybridization and selective polymer fractionation

21 May 2023

58

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Direct covalent mercuration of nucleotides and polynucleotides

21 May 2023

59

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Preparation and properties of S-cyano derivatives of creatine kinase

21 May 2023

61

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The reaction of p-mercuribenzoate with methemerythrin

21 May 2023

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A colorimetric method for determining low concentrations of mercaptans

21 May 2023

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21 May 2023

Tissue sulfhydryl groups

68

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Specific chemical modification of proteins

21 May 2023

76

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Determination of sulfhydryl groups with 2,2′- or 4,4′-dithiodipyridine

21 May 2023

79

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The use of 2,2'-dithiobis-(5-nitropyridine) as a selective reagent for the detection of thiols

21 May 2023

80

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The determination of thiols and of total glutathione in human blood using 6,6′-dithiodinicotinic acid (CPDS)

21 May 2023

81

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[37] Reaction of protein sulfhydryl groups with Ellman's reagent

21 May 2023

85

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Use of methyl iodide for probing the polarity of the immediate environment of --SH groups in thiolenzymes. Reaction of methyl iodide with thiosubtilisin

21 May 2023

86

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Effect of the Immediate Environment on the Reactivity of the Essential-SH Group of Papain

21 May 2023

87

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The Synthesis of Some New Cysteine-Containing Unsymmetrical Disulfides

21 May 2023

88

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Conformational changes of purified (Na+ + K+)-ATPase detected by a sulfhydryl fluorescence probe

21 May 2023

89

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5-Iodoacetamidofluorescein-labeled chloroplast coupling factor 1: conformational dynamics and labeling-site characterization

21 May 2023

90

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Conformational flexibility and structure of creatine kinase

21 May 2023

91

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p-Azidophenacyl bromide, a versatile photolabile bifunctional reagent. Reaction with glyceraldehyde-3-phosphate dehydrogenase

21 May 2023

93

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Spin-labeling as a general method in studying antibody active site

21 May 2023

95

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Synthesis and characterization of two fluorescent sulfhydryl reagents

21 May 2023

96

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19 Saturation-transfer spectroscopy

21 May 2023

97

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Librational motion of an "immobilized" spin label: hemoglobin spin labeled by a maleimide derivative

21 May 2023

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Spin-labelled phosphofructokinase and its interactions with ATP and metal-ATP complexes as studied by magnetic-resonance methods.

21 May 2023

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17 The spin-labeling technique

21 May 2023

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Novel sulfhydryl reagents

21 May 2023

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Catalytic oxidation of sulfhydryl groups by o-phenanthroline copper complex.

21 May 2023

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Reversible inactivation of rabbit muscle aldolase by ophenanthroline

21 May 2023

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Spectroscopic and immunochemical studies with nitrobenzoxadiazolealanine, a fluorescent dinitrophenyl analogue

21 May 2023

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Characterization of hydrophobic regions in proteins by spin labeling technique.

21 May 2023

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Spectrochemical and ligand-binding studies of an active mercurinitrophenol-labeled creatine kinase

21 May 2023

114

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A sulfhydryl-specific fluorescent label, S-mercuric N-dansylcysteine. Titrations of glutathione and muscle proteins

21 May 2023

115

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Solute quenching of protein fluorescence

21 May 2023

116

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The oxidation of beta-lactoglobulin in sodium dodecyl sulfate solutions

21 May 2023

117

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Ionization of a nitrophenol-containing reporter group at the active site of papain

21 May 2023

118

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Potentiometric determination of ionizations at the active site of papain

21 May 2023

119

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Preparation of fully active ficin from Ficus glabrata by covalent chromatography and characterization of its active centre by using 2,2'-depyridyl disulphide as a reactivity probe

21 May 2023

122

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Intramolecular Inhibition by Enzyme of Site-Specific Modification Reactions can Mask pKa Values Characteristic of the Reaction Pathway: Do the Side Chains of Aspartic Acid-158 and Lysine-156 of Papain Form an Ion-Pair?

21 May 2023

123

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Studies on thiol proteinases with fluorescent probes [proceedings]

21 May 2023

124

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Spin-labeled hemoglobin derivatives in solution, polycrystalline suspensions, and single crystals

21 May 2023

125

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A new class of chromophoric organomercurials and their reactions with d-glyceraldehyde 3-phosphate dehydrogenase

21 May 2023

126

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Nuclear relaxation measurements of the geometry of enzyme-bound substrates and analogs.

21 May 2023

128

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Number and location of adenosine triphosphatase sites of myosin

21 May 2023

131

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Synthesis of 6-mercapto-9-beta-D-ribofuranosylpurine 5'-triphosphate, a sulfhydryl analog of ATP

21 May 2023

132

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Reversible modification of the sulfhydryl groups of Escherichia coli succinic thiokinase with methanethiolating reagents, 5,5′-dithio-bis(2-nitrobenzoic acid), p-hydroxymercuribenzoate, and ethylmercurithiosalicylate

21 May 2023

133

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A convenient method of preparation of high-activity urease from Canavalia ensiformis by covalent chromatography and an investigation of its thiol groups with 2,2'-dipyridyl disulphide as a thiol titrant and reactivity probe

21 May 2023

134

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INTERACTION OF THE RADICAL ION OF CHLORPROMAZINE WITH DEOXYRIBONUCLEIC ACID

21 May 2023

137

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Chemical Cross-Linking: Reagents and Problems in Studies of Membrane Structure

21 May 2023

142

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https://api.crossref.org/works/10.1016/0020-711X(79)90088-0

A chromatographic method of analysis for thiols

21 May 2023

145

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Inhibition of rabbit muscle creatine kinase by iodomethane [proceedings]

21 May 2023

148

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[36] Reactions with N-ethylmaleimide and p-mercuribenzoate

21 May 2023

150

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Structural properties of the creatine-kinase active site studied by chromophoric-reagent labelling.

21 May 2023

151

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Alkylation of cysteine thiols with 1,3-propane sultone

21 May 2023

152

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Selective chemical modification of proteins

21 May 2023

156

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In vitro oxidation of intrinsic sulfhydryl groups yields polymers of the two predominant polypeptides in the nuclear envelope fraction

21 May 2023

157

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Benzofuroxan as a thiol-specific reactivity probe. Kinetics of its reactions with papain, ficin, bromelain and low-molecular-weight thiols

21 May 2023

158

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Characterization of the papain active centre by using two-protonic-state electrophiles as reactivity probes. Evidence for nucleophilic reactivity in the un-interrupted cysteine-25-histidine-159 interactive system

21 May 2023

159

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Evaluation of benzofuroxan as a chromophoric oxidizing agent for thiol groups by using its reactions with papain, ficin, bromelain and low-molecular-weight thiols

21 May 2023

161

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https://api.crossref.org/works/10.1016/0020-711X(79)90088-0

An agarose mercurial column for the separation of mercaptopapain and nonmercaptopapain

21 May 2023

162

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https://api.crossref.org/works/10.1016/0020-711X(79)90088-0

Proton Equilibria in the Binding of Zn2+ and of Methylmercuric Iodide to Papain

21 May 2023

163

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Simple alkanethiol groups for temporary blocking of sulfhydryl groups of enzymes

21 May 2023

165

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Reactions of N-ethylmaleimide with peptides and amino acids

21 May 2023

167

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https://api.crossref.org/works/10.1016/0020-711X(79)90088-0

Electron microscopic location of protein thiol residues

21 May 2023

170

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https://api.crossref.org/works/10.1016/0020-711X(79)90088-0

Fluorescence Energy Transfer as a Spectroscopic Ruler

21 May 2023

171

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A reporter group delivery system with both absolute and selective specificity for thiol groups and an improved fluorescent probe containing the 7-nitrobenzo-2-oxa-1,3-diazole moiety

21 May 2023

172

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The gross architecture of an antibody-combining site as determined by spin-label mapping

21 May 2023

173

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https://api.crossref.org/works/10.1016/0020-711X(79)90088-0

Specific isolation of cysteine peptides by covalent chromatography on thiol agarose derivatives

21 May 2023

175

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https://api.crossref.org/works/10.1016/0020-711X(79)90088-0

Fluorine nuclear magnetic resonance studies of proteins

21 May 2023

177

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Magnetic resonance studies of spin-labeled creatine kinase system and interaction of two paramagnetic probes

21 May 2023

179

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https://api.crossref.org/works/10.1016/0020-711X(79)90088-0

The reactivity of the sulfhydryl groups of lobster muscle glyceraldehyde 3-phosphate dehydrogenase

21 May 2023

184

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https://api.crossref.org/works/10.1016/0020-711X(79)90088-0

Uses of Fluorescence in Biophysics: Some Recent Developments

21 May 2023

186

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Interaction of a spin-labeled analog of acetyl coenzyme A with citrate synthase. Paramagnetic resonance and proton relaxation rate studies of binary and ternary complexes

21 May 2023

187

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Comparison of the dimensions of the combining sites of the dinitrophenyl-binding immunoglobulin A myeloma proteins MOPC 315, MOPC 460 and XRPC 25 by spin-label mapping

21 May 2023

191

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[57] Bifunctional reagents

21 May 2023

193

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Kinetics of the reaction of 1,3-dihydro-1-hydroxy-3-oxo-1,2-benziodoxole with cysteine

21 May 2023

194

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N-(1-Pyrene)maleimide: a fluorescent crosslinking reagent

21 May 2023

196

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Use of a distant reporter group as evidence for a conformational change in a sensory receptor

21 May 2023

198

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21 May 2023

Identifiers

1 reference

7 October 2017

1 reference