(Q43000793)

12 November 2017

title

Efficient amplification and cloning of near full-length hepatitis C virus genome from clinical samples (English)

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12 November 2017

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Adrian M. Di Bisceglie

series ordinal

3

object named as

Adrian M Di Bisceglie

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12 November 2017

Xiaofeng Fan

1

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12 November 2017

Yanjuan Xu

series ordinal

2

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12 November 2017

language of work or name

English

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publication date

14 June 2006

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Biochemical and Biophysical Research Communications

12 November 2017

published in

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12 November 2017

volume

346

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12 November 2017

issue

4

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12 November 2017

page(s)

1163-1172

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New specificity and yield enhancer of polymerase chain reactions

12 November 2017

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12 December 2020

Accurate representation of the hepatitis C virus quasispecies in 5.2-kilobase amplicons

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Detailed mapping of RNA secondary structures in core and NS5B-encoding region sequences of hepatitis C virus by RNase cleavage and novel bioinformatic prediction methods.

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Oligonucleotide inhibitors of Taq DNA polymerase facilitate detection of low copy number targets by PCR.

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12 December 2020

Quasispecies heterogeneity within the E1/E2 region as a pretreatment variable during pegylated interferon therapy of chronic hepatitis C virus infection.

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Thermodynamic and phylogenetic prediction of RNA secondary structures in the coding region of hepatitis C virus

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12 December 2020

A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences

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12 December 2020

Visualization of hepatitis C virions and putative defective interfering particles isolated from low-density lipoproteins

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12 December 2020

Phosphorothioate primers improve the amplification of DNA sequences by DNA polymerases with proofreading activity

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12 December 2020

Transcripts from a single full-length cDNA clone of hepatitis C virus are infectious when directly transfected into the liver of a chimpanzee

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12 December 2020

Stimulation and suppression of PCR-mediated recombination

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12 December 2020

Effective amplification of 20-kb DNA by reverse transcription PCR.

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12 December 2020

Betaine improves the PCR amplification of GC-rich DNA sequences

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12 December 2020

Optimizing multiplex and LA-PCR with betaine

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12 December 2020

RNase H and its effects on PCR

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12 December 2020

Defective interfering particles and virus evolution.

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12 December 2020

PCR amplification of up to 35-kb DNA with high fidelity and high yield from lambda bacteriophage templates

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12 December 2020

A refined long RT-PCR technique to amplify complete viral RNA genome sequences from clinical samples: application to a novel hepatitis C virus variant of genotype 6.

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12 December 2020

The neighbor-joining method: a new method for reconstructing phylogenetic trees

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12 December 2020

Effective amplification of long targets from cloned inserts and human genomic DNA

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12 December 2020

Minimizing DNA recombination during long RT-PCR

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12 December 2020

Transmission of hepatitis C by intrahepatic inoculation with transcribed RNA

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12 December 2020

Reevaluation of a North India isolate of hepatitis E virus based on the full-length genomic sequence obtained following long RT-PCR.

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12 December 2020

Long PCR and its application to hepatitis viruses: amplification of hepatitis A, hepatitis B, and hepatitis C virus genomes

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Inhibition of multiple thermostable DNA polymerases by a heterodimeric aptamer

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Long RT-PCR of the entire 8.5-kb NF1 open reading frame and mutation detection on agarose gels

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Single base extension (SBE) with proofreading polymerases and phosphorothioate primers: improved fidelity in single-substrate assays

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Amplimers with 3'-terminal phosphorothioate linkages resist degradation by vent polymerase and reduce Taq polymerase mispriming.

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Analysis of ORF 1 in European porcine reproductive and respiratory syndrome virus by long RT-PCR and restriction fragment length polymorphism (RFLP) analysis

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12 December 2020

Complete replication of hepatitis C virus in cell culture

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12 December 2020

Reverse transcriptase can inhibit PCR and stimulate primer-dimer formation

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12 December 2020

Differential priming of RNA templates during cDNA synthesis markedly affects both accuracy and reproducibility of quantitative competitive reverse-transcriptase PCR.

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12 December 2020

Amplification of a full-length Borna disease virus (BDV) cDNA from total RNA of cells persistently infected with BDV.

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12 December 2020

Sequence analysis of hepatitis C virus isolated from a fulminant hepatitis patient

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12 December 2020

Rapid RT-PCR amplification of full-length poliovirus genomes allows rapid discrimination between wild-type and recombinant vaccine-derived polioviruses

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Differential amplification of hypervariable region 1 of hepatitis C virus by partially mismatched primers

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12 December 2020

Detection of genome-scale ordered RNA structure (GORS) in genomes of positive-stranded RNA viruses: Implications for virus evolution and host persistence

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12 December 2020

Cloning and characterization of a complete open reading frame of the hepatitis C virus genome in only two cDNA fragments

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12 December 2020

Production of infectious hepatitis C virus in tissue culture from a cloned viral genome

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12 December 2020

The complete consensus sequence of coxsackievirus B6 and generation of infectious clones by long RT-PCR.

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12 December 2020

Full-Length cDNA Synthesis for Long-Distance RT-PCR of Large mRNA Transcripts

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Long RT-PCR Amplification of the entire coding sequence of the polycystic kidney disease 1 (PKD1) gene.

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12 December 2020

Amplification and fusion of long fragments of hepatitis C virus genome

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12 December 2020

Robust hepatitis C virus infection in vitro

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12 December 2020

The outcome of acute hepatitis C predicted by the evolution of the viral quasispecies

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12 December 2020

Factors affecting PCR-mediated recombination.

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12 December 2020

A strategy for obtaining near full-length HCV cDNA clones (assemblicons) by assembly PCR.

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12 December 2020

10.1016/J.BBRC.2006.06.039

Identifiers

DOI

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12 November 2017

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