(Q48007914)

7 February 2018

title

Contribution of surface salt bridges to protein stability: guidelines for protein engineering (English)

1 reference

7 February 2018

author name string

George I Makhatadze

1

1 reference

7 February 2018

Vakhtang V Loladze

2

1 reference

7 February 2018

Dmitri N Ermolenko

3

1 reference

7 February 2018

XiaoFen Chen

4

1 reference

7 February 2018

Susan T Thomas

5

1 reference

7 February 2018

language of work or name

English

0 references

publication date

1 April 2003

1 reference

7 February 2018

Journal of Molecular Biology

published in

1 reference

7 February 2018

volume

327

1 reference

7 February 2018

issue

5

1 reference

7 February 2018

page(s)

1135-1148

1 reference

7 February 2018

Electrostatic effects in proteins

cites work

1 reference

https://api.crossref.org/works/10.1016%2FS0022-2836%2803%2900233-X

Conformational equilibria in -and -chymotrypsin. The energetics and importance of the salt bridge

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FS0022-2836%2803%2900233-X

The Influence of a Single Salt Bridge on Static and Dynamic Features of the Globular Solution Conformation of the Basic Pancreatic Trypsin Inhibitor. 1H and 13C Nuclear-Magnetic-Resonance Studies of the Native and the Transaminated Inhibitor

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FS0022-2836%2803%2900233-X

A salt bridge stabilizes the helix formed by isolated C-peptide of RNase A

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FS0022-2836%2803%2900233-X

The energetics of ion-pair and hydrogen-bonding interactions in a helical peptide

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FS0022-2836%2803%2900233-X

Structural and genetic analysis of protein stability

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FS0022-2836%2803%2900233-X

Contributions of a hydrogen bond/salt bridge network to the stability of secondary and tertiary structure in lambda repressor

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FS0022-2836%2803%2900233-X

Structural and genetic analysis of the folding and function of T4 lysozyme

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FS0022-2836%2803%2900233-X

Electrostatic Interactions across a β-Sheet†

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FS0022-2836%2803%2900233-X

Ion-pair and charged hydrogen-bond interactions between histidine and aspartate in a peptide helix

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FS0022-2836%2803%2900233-X

Contribution of a salt bridge to the thermostability of DNA binding protein HU from Bacillus stearothermophilus determined by site-directed mutagenesis

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FS0022-2836%2803%2900233-X

Putative interhelix ion pairs involved in the stability of myoglobin

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FS0022-2836%2803%2900233-X

Sidechain interactions in parallel beta sheets: the energetics of cross-strand pairings

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FS0022-2836%2803%2900233-X

Contribution of surface salt bridges to protein stability

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FS0022-2836%2803%2900233-X

Contribution of engineered electrostatic interactions to the stability of cytosolic malate dehydrogenase

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FS0022-2836%2803%2900233-X

Evaluation of the energetic contribution of an ionic network to beta-sheet stability

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FS0022-2836%2803%2900233-X

pH-induced denaturation of proteins: a single salt bridge contributes 3-5 kcal/mol to the free energy of folding of T4 lysozyme

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FS0022-2836%2803%2900233-X

Importance of two buried salt bridges in the stability and folding pathway of barnase.

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FS0022-2836%2803%2900233-X

Strength and co-operativity of contributions of surface salt bridges to protein stability

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FS0022-2836%2803%2900233-X

Estimating the contribution of engineered surface electrostatic interactions to protein stability by using double-mutant cycles

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FS0022-2836%2803%2900233-X

Effects of engineered salt bridges on the stability of subtilisin BPN'

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FS0022-2836%2803%2900233-X

Contributions of engineered surface salt bridges to the stability of T4 lysozyme determined by directed mutagenesis

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FS0022-2836%2803%2900233-X

Surface electrostatic interactions contribute little of stability of barnase

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FS0022-2836%2803%2900233-X

Contribution of a proline residue and a salt bridge to the stability of a type I reverse turn in chymotrypsin inhibitor-2

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FS0022-2836%2803%2900233-X

The net energetic contribution of interhelical electrostatic attractions to coiled-coil stability

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FS0022-2836%2803%2900233-X

Protein destabilization by electrostatic repulsions in the two-stranded alpha-helical coiled-coil/leucine zipper

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FS0022-2836%2803%2900233-X

Design of heterotetrameric coiled coils: evidence for increased stabilization by Glu(-)-Lys(+) ion pair interactions

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FS0022-2836%2803%2900233-X

Investigation of electrostatic interactions in two-stranded coiled-coils through residue shuffling

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FS0022-2836%2803%2900233-X

Inter-helical interactions in the leucine zipper coiled coil dimer: pH and salt dependence of coupling energy between charged amino acids

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FS0022-2836%2803%2900233-X

Evaluation of the energetic contribution of interhelical Coulombic interactions for coiled coil helix orientation specificity

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FS0022-2836%2803%2900233-X

Contribution of buried lysine residues to the oligomerization specificity and stability of the fos coiled coil

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FS0022-2836%2803%2900233-X

Measurement of interhelical electrostatic interactions in the GCN4 leucine zipper

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FS0022-2836%2803%2900233-X

Electrostatic effects in myoglobin. Application of the modified Tanford-Kirkwood theory to myoglobins from horse, California grey whale, harbor seal, and California sea lion

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FS0022-2836%2803%2900233-X

Contributions of individual amino acid residues to the structural stability of cetacean myoglobins.

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FS0022-2836%2803%2900233-X

Calculation of electrostatic interactions in proteins

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FS0022-2836%2803%2900233-X

Energetics of charge-charge interactions in proteins.

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FS0022-2836%2803%2900233-X

Charge effects on folded and unfolded proteins

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FS0022-2836%2803%2900233-X

pKa's of ionizable groups in proteins: atomic detail from a continuum electrostatic model

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FS0022-2836%2803%2900233-X

Electrostatic contributions to the stability of halophilic proteins

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FS0022-2836%2803%2900233-X

The stability of salt bridges at high temperatures: implications for hyperthermophilic proteins

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FS0022-2836%2803%2900233-X

Generalized born models of macromolecular solvation effects

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FS0022-2836%2803%2900233-X

Do salt bridges stabilize proteins? A continuum electrostatic analysis.

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FS0022-2836%2803%2900233-X

Classical electrostatics in biology and chemistry

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FS0022-2836%2803%2900233-X

Electrostatic contributions to the stability of hyperthermophilic proteins.

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FS0022-2836%2803%2900233-X

Are buried salt bridges important for protein stability and conformational specificity?

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FS0022-2836%2803%2900233-X

Protein stabilization by removal of unsatisfied polar groups: computational approaches and experimental tests

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FS0022-2836%2803%2900233-X

Direct measurement of salt-bridge solvation energies using a peptide model system: implications for protein stability

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FS0022-2836%2803%2900233-X

Electrostatic strengths of salt bridges in thermophilic and mesophilic glutamate dehydrogenase monomers.

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FS0022-2836%2803%2900233-X

Salt bridge stability in monomeric proteins.

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FS0022-2836%2803%2900233-X

Fluctuations in ion pairs and their stabilities in proteins

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FS0022-2836%2803%2900233-X

Relationship between ion pair geometries and electrostatic strengths in proteins

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FS0022-2836%2803%2900233-X

Electrostatic contributions to T4 lysozyme stability: solvent-exposed charges versus semi-buried salt bridges

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FS0022-2836%2803%2900233-X

7 January 2021

Ion-pairs in proteins

1 reference

https://api.crossref.org/works/10.1016%2FS0022-2836%2803%2900233-X

Protein backbone angle restraints from searching a database for chemical shift and sequence homology

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FS0022-2836%2803%2900233-X

Electrostatic interactions in ubiquitin: stabilization of carboxylates by lysine amino groups.

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FS0022-2836%2803%2900233-X

Measuring and increasing protein stability

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FS0022-2836%2803%2900233-X

A test of the linear extrapolation of unfolding free energy changes over an extended denaturant concentration range

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FS0022-2836%2803%2900233-X

The use of double mutants to detect structural changes in the active site of the tyrosyl-tRNA synthetase (Bacillus stearothermophilus)

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FS0022-2836%2803%2900233-X

Effects of site-specific amino acid modification on protein interactions and biological function

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FS0022-2836%2803%2900233-X

Double-mutant cycles: a powerful tool for analyzing protein structure and function

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FS0022-2836%2803%2900233-X

Accommodation of insertion mutations on the surface and in the interior of staphylococcal nuclease

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FS0022-2836%2803%2900233-X

Crystal structures of mutant forms of the Bacillus caldolyticus cold shock protein differing in thermal stability

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FS0022-2836%2803%2900233-X

A structural double-mutant cycle: estimating the strength of a buried salt bridge in barnase

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FS0022-2836%2803%2900233-X

Contribution of salt bridges near the surface of a protein to the conformational stability

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FS0022-2836%2803%2900233-X

Hydrophobic interactions at the Ccap position of the C-capping motif of alpha-helices

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FS0022-2836%2803%2900233-X

Engineering a thermostable protein via optimization of charge-charge interactions on the protein surface

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FS0022-2836%2803%2900233-X

To charge or not to charge?

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FS0022-2836%2803%2900233-X

Protein stability and electrostatic interactions between solvent exposed charged side chains

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FS0022-2836%2803%2900233-X

Increasing protein stability by altering long-range coulombic interactions

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FS0022-2836%2803%2900233-X

Rational Modification of Protein Stability by the Mutation of Charged Surface Residues†

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FS0022-2836%2803%2900233-X

Two exposed amino acid residues confer thermostability on a cold shock protein

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FS0022-2836%2803%2900233-X

Electrostatic stabilization of a thermophilic cold shock protein

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FS0022-2836%2803%2900233-X

The effects of ionic strength on protein stability: the cold shock protein family

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FS0022-2836%2803%2900233-X

Distance dependence and salt sensitivity of pairwise, coulombic interactions in a protein

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FS0022-2836%2803%2900233-X

Charge-charge interactions are the primary determinants of the pK values of the ionizable groups in Ribonuclease T1

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FS0022-2836%2803%2900233-X

Changes in stability upon charge reversal and neutralization substitution in staphylococcal nuclease are dominated by favorable electrostatic effects

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FS0022-2836%2803%2900233-X

Thermal versus guanidine-induced unfolding of ubiquitin. An analysis in terms of the contributions from charge-charge interactions to protein stability

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FS0022-2836%2803%2900233-X

Comparing the polarities of the amino acids: side-chain distribution coefficients between the vapor phase, cyclohexane, 1-octanol, and neutral aqueous solution

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FS0022-2836%2803%2900233-X

Solvation energies of amino acid side chains and backbone in a family of host-guest pentapeptides.

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FS0022-2836%2803%2900233-X

Analysis of the interaction between charged side chains and the alpha-helix dipole using designed thermostable mutants of phage T4 lysozyme

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FS0022-2836%2803%2900233-X

Hydration of the peptide backbone largely defines the thermodynamic propensity scale of residues at the C' position of the C-capping box of alpha-helices

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FS0022-2836%2803%2900233-X

Anion binding to the ubiquitin molecule

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FS0022-2836%2803%2900233-X

Correction of light‐scattering errors in spectrophotometric protein determinations

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FS0022-2836%2803%2900233-X

Instrumentation for automated determination of protein stability

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FS0022-2836%2803%2900233-X

Interpretation of protein titration curves. Application to lysozyme

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FS0022-2836%2803%2900233-X

Thermodynamic study of the acid denaturation of barnase and its dependence on ionic strength: evidence for residual electrostatic interactions in the acid/thermally denatured state

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FS0022-2836%2803%2900233-X

Perturbed pKA-values in the denatured states of proteins

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FS0022-2836%2803%2900233-X

pKA values of carboxyl groups in the native and denatured states of barnase: the pKA values of the denatured state are on average 0.4 units lower than those of model compounds

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FS0022-2836%2803%2900233-X

Hydrogen bonds and the pH dependence of ovomucoid third domain stability

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FS0022-2836%2803%2900233-X

Realistic modeling of the denatured states of proteins allows accurate calculations of the pH dependence of protein stability

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FS0022-2836%2803%2900233-X

pKaValues and the pH Dependent Stability of the N-Terminal Domain of L9 as Probes of Electrostatic Interactions in the Denatured State. Differentiation between Local and Nonlocal Interactions†

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FS0022-2836%2803%2900233-X

Charge-charge interactions influence the denatured state ensemble and contribute to protein stability

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FS0022-2836%2803%2900233-X

pH dependence of stability of staphylococcal nuclease: evidence of substantial electrostatic interactions in the denatured state

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FS0022-2836%2803%2900233-X

A Gaussian-chain model for treating residual charge-charge interactions in the unfolded state of proteins

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FS0022-2836%2803%2900233-X

Studies on protein stability with T4 lysozyme

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FS0022-2836%2803%2900233-X

inferred from DOI database lookup

7 January 2021

based on heuristic

pH-dependent processes in proteins.

1 reference

https://api.crossref.org/works/10.1016%2FS0022-2836%2803%2900233-X

inferred from DOI database lookup

7 January 2021

based on heuristic

Identifiers

DOI

10.1016/S0022-2836(03)00233-X

1 reference

7 February 2018

1 reference

7 February 2018