(Q51551994)

6 April 2018

title

Recombineering, transfection, Western, IP and ChIP methods for protein tagging via gene targeting or BAC transgenesis. (English)

1 reference

6 April 2018

Konstantinos Anastassiadis

1 reference

8

1 reference

6 April 2018

author name string

Helmut Hofemeister

1

1 reference

6 April 2018

Giovanni Ciotta

2

1 reference

6 April 2018

Jun Fu

3

1 reference

6 April 2018

Philipp Martin Seibert

4

1 reference

6 April 2018

Alexander Schulz

5

1 reference

6 April 2018

Marcello Maresca

6

1 reference

6 April 2018

Mihail Sarov

7

1 reference

6 April 2018

A Francis Stewart

9

1 reference

6 April 2018

publication date

31 December 2010

1 reference

6 April 2018

published in

Methods

1 reference

6 April 2018

volume

53

1 reference

6 April 2018

page(s)

437-452

1 reference

6 April 2018

issue

4

1 reference

6 April 2018

Dre recombinase, like Cre, is a highly efficient site-specific recombinase in E. coli, mammalian cells and mice

cites work

1 reference

https://api.crossref.org/works/10.1016%2FJ.YMETH.2010.12.026

Simplified generation of targeting constructs using ET recombination

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FJ.YMETH.2010.12.026

Pooling and PCR as a method to combat low frequency gene targeting in mouse embryonic stem cells

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FJ.YMETH.2010.12.026

Toward a comprehensive atlas of the physical interactome of Saccharomyces cerevisiae

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FJ.YMETH.2010.12.026

Recombineering: a powerful new tool for mouse functional genomics

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FJ.YMETH.2010.12.026

Genetic engineering using homologous recombination

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FJ.YMETH.2010.12.026

Efficient biotinylation and single-step purification of tagged transcription factors in mammalian cells and transgenic mice

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FJ.YMETH.2010.12.026

One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FJ.YMETH.2010.12.026

The DNA intermediate in yeast Ty1 element transposition copurifies with virus-like particles: cell-free Ty1 transposition

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FJ.YMETH.2010.12.026

High efficiency mutagenesis, repair, and engineering of chromosomal DNA using single-stranded oligonucleotides

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FJ.YMETH.2010.12.026

Positive and negative roles of an initiator protein at an origin of replication

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FJ.YMETH.2010.12.026

A recombineering pipeline to make conditional targeting constructs

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FJ.YMETH.2010.12.026

Proteome survey reveals modularity of the yeast cell machinery

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FJ.YMETH.2010.12.026

Global landscape of protein complexes in the yeast Saccharomyces cerevisiae

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FJ.YMETH.2010.12.026

PCR-mediated gene replacement in Escherichia coli

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FJ.YMETH.2010.12.026

Rapid modification of bacterial artificial chromosomes by ET-recombination.

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FJ.YMETH.2010.12.026

Point mutation of bacterial artificial chromosomes by ET recombination

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FJ.YMETH.2010.12.026

Techniques: Recombinogenic engineering--new options for cloning and manipulating DNA.

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FJ.YMETH.2010.12.026

A variant of yellow fluorescent protein with fast and efficient maturation for cell-biological applications

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FJ.YMETH.2010.12.026

Agouti C57BL/6N embryonic stem cells for mouse genetic resources

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FJ.YMETH.2010.12.026

The S. cerevisiae SET3 complex includes two histone deacetylases, Hos2 and Hst1, and is a meiotic-specific repressor of the sporulation gene program

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FJ.YMETH.2010.12.026

BAC TransgeneOmics: a high-throughput method for exploration of protein function in mammals

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FJ.YMETH.2010.12.026

A generic protein purification method for protein complex characterization and proteome exploration

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FJ.YMETH.2010.12.026

A recombineering pipeline for functional genomics applied to Caenorhabditis elegans.

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FJ.YMETH.2010.12.026

Chromatin Central: towards the comparative proteome by accurate mapping of the yeast proteomic environment

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FJ.YMETH.2010.12.026

Development of 2A peptide-based strategies in the design of multicistronic vectors

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FJ.YMETH.2010.12.026

Overview of tag protein fusions: from molecular and biochemical fundamentals to commercial systems

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FJ.YMETH.2010.12.026

Engineering the mouse genome with bacterial artificial chromosomes to create multipurpose alleles.

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FJ.YMETH.2010.12.026

High-throughput engineering of the mouse genome coupled with high-resolution expression analysis

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FJ.YMETH.2010.12.026

Comparative assessment of large-scale data sets of protein-protein interactions

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FJ.YMETH.2010.12.026

Efficient and rapid affinity purification of proteins using recombinant fusion proteases

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FJ.YMETH.2010.12.026

An improved recombineering approach by adding RecA to lambda Red recombination

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FJ.YMETH.2010.12.026

A protocol for constructing gene targeting vectors: generating knockout mice for the cadherin family and beyond.

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FJ.YMETH.2010.12.026

Site-specific gene targeting in mouse embryonic stem cells with intact bacterial artificial chromosomes.

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FJ.YMETH.2010.12.026

An efficient recombination system for chromosome engineering in Escherichia coli

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FJ.YMETH.2010.12.026

A new logic for DNA engineering using recombination in Escherichia coli

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FJ.YMETH.2010.12.026

Reduction of liver Fas expression by an antisense oligonucleotide protects mice from fulminant hepatitis

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FJ.YMETH.2010.12.026

10.1016/J.YMETH.2010.12.026

7 January 2021

Identifiers

DOI

1 reference

6 April 2018

PubMed ID

21195765

1 reference

6 April 2018