(Q52850214)

8 May 2018

title

Identifying and characterizing substrates of the RNase E/G family of enzymes. (English)

1 reference

8 May 2018

Kenneth J McDowall

3

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author name string

Louise Kime

series ordinal

1

1 reference

8 May 2018

Stefanie S Jourdan

series ordinal

2

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8 May 2018

language of work or name

English

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publication date

1 January 2008

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8 May 2018

published in

Methods in Enzymology

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8 May 2018

volume

447

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8 May 2018

page(s)

215-241

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8 May 2018

Mechanism of RNA silencing by Hfq-binding small RNAs

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The Ams (altered mRNA stability) protein and ribonuclease E are encoded by the same structural gene of Escherichia coli

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Ectopic RNase E sites promote bypass of 5'-end-dependent mRNA decay in Escherichia coli.

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The quaternary structure of RNase G from Escherichia coli

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"Zn-link": a metal-sharing interface that organizes the quaternary structure and catalytic site of the endoribonuclease, RNase E.

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TheEscherichia coliRNA degradosome: structure, function and relationship to other ribonucleolytic multienyzme complexes

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Copurification of E. coli RNAase E and PNPase: evidence for a specific association between two enzymes important in RNA processing and degradation.

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Initiation of RNA decay in Escherichia coli by 5' pyrophosphate removal

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An intercistronic stem-loop structure functions as an mRNA decay terminator necessary but insufficient for puf mRNA stability

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RNase E: still a wonderfully mysterious enzyme.

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Processing of the Bacillus subtilis thrS leader mRNA is RNase E-dependent in Escherichia coli.

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7 January 2021

RNase E activity is conferred by a single polypeptide: overexpression, purification, and properties of the ams/rne/hmp1 gene product

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7 January 2021

Critical features of a conserved RNA stem-loop important for feedback regulation of RNase E synthesis

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7 January 2021

Specificity of Escherichia coli endoribonuclease RNase E: in vivo and in vitro analysis of mutants in a bacteriophage T4 mRNA processing site.

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7 January 2021

A 5'-terminal stem-loop structure can stabilize mRNA in Escherichia coli

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7 January 2021

The catalytic domain of RNase E shows inherent 3' to 5' directionality in cleavage site selection

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7 January 2021

Structural analysis and in vitro processing to p5 rRNA of a 9S RNA molecule isolated from an rne mutant of E. coli

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7 January 2021

An mRNA degrading complex in Rhodobacter capsulatus.

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RNase E autoregulates its synthesis by controlling the degradation rate of its own mRNA in Escherichia coli: unusual sensitivity of the rne transcript to RNase E activity.

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7 January 2021

Catalytic activation of multimeric RNase E and RNase G by 5'-monophosphorylated RNA

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7 January 2021

Sensing of 5' monophosphate by Escherichia coli RNase G can significantly enhance association with RNA and stimulate the decay of functional mRNA transcripts in vivo

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7 January 2021

Probing the substrate specificity of Escherichia coli RNase E using a novel oligonucleotide-based assay

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7 January 2021

Characterization of Aquifex aeolicus RNase E/G.

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7 January 2021

Enhanced cleavage of RNA mediated by an interaction between substrates and the arginine-rich domain of E. coli ribonuclease E 1 1Edited by I. B. Holland

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7 January 2021

Extensive overproduction of the AdhE protein by rng mutations depends on mutations in the cra gene or in the Cra-box of the adhE promoter

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RNase G-dependent degradation of the eno mRNA encoding a glycolysis enzyme enolase in Escherichia coli

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7 January 2021

RNase E polypeptides lacking a carboxyl-terminal half suppress a mukB mutation in Escherichia coli

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7 January 2021

Combined actions of multiple hairpin loop structures and sites of rate-limiting endonucleolytic cleavage determine differential degradation rates of individual segments within polycistronic puf operon mRNA

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7 January 2021

The rate of decay of Rhodobacter capsulatus-specific puf mRNA segments is differentially affected by RNase E activity in Escherichia coli.

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7 January 2021

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RNase G complementation of rne null mutation identifies functional interrelationships with RNase E in Escherichia coli

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7 January 2021

A Streptomyces coelicolor functional orthologue of Escherichia coli RNase E shows shuffling of catalytic and PNPase-binding domains

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7 January 2021

RNase G (CafA protein) and RNase E are both required for the 5' maturation of 16S ribosomal RNA.

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7 January 2021

RNase E plays an essential role in the maturation of Escherichia coli tRNA precursors

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7 January 2021

The rate of processing and degradation of antisense RNAI regulates the replication of ColE1-type plasmids in vivo

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7 January 2021

RNase E is required for the maturation of ssrA RNA and normal ssrA RNA peptide-tagging activity

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7 January 2021

Gene 32 transcription and mRNA processing in T4-related bacteriophages

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7 January 2021

Specific endonucleolytic cleavage of the mRNA for ribosomal protein S20 of Escherichia coli requires the product of the ams gene in vivo and in vitro

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7 January 2021

Ribonuclease E is a 5'-end-dependent endonuclease

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7 January 2021

The role of RNA structure in determining RNase E-dependent cleavage sites in the mRNA for ribosomal protein S20 in vitro

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7 January 2021

The RNA degradosome: life in the fast lane of adaptive molecular evolution.

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7 January 2021

The N-terminal domain of the rne gene product has RNase E activity and is non-overlapping with the arginine-rich RNA-binding site

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7 January 2021

The ams-1 and rne-3071 temperature-sensitive mutations in the ams gene are in close proximity to each other and cause substitutions within a domain that resembles a product of the Escherichia coli mre locus

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7 January 2021

Site-specific RNase E cleavage of oligonucleotides and inhibition by stem-loops

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7 January 2021

Genetic studies of cleavage-initiated mRNA decay and processing of ribosomal 9S RNA show that the Escherichia coli ams and rne loci are the same

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7 January 2021

Proteins associated with RNase E in a multicomponent ribonucleolytic complex

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7 January 2021

Escherichia coli endoribonuclease RNase E: autoregulation of expression and site-specific cleavage of mRNA

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7 January 2021

RNase E, an endoribonuclease, has a general role in the chemical decay of Escherichia coli mRNA: evidence that rne and ams are the same genetic locus

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7 January 2021

Differential mRNA stability controls relative gene expression within a polycistronic operon

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7 January 2021

Stabilization of translationally active mRNA by prokaryotic REP sequences

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7 January 2021

Mutations affecting mRNA processing and fimbrial biogenesis in the Escherichia coli pap operon

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7 January 2021

A conditional lethal mutation in an Escherichia coli strain with a longer chemical lifetime of messenger RNA

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7 January 2021

Initiation of tRNA maturation by RNase E is essential for cell viability in E. coli

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7 January 2021

Distinct populations of primary and secondary effectors during RNAi in C. elegans

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7 January 2021

Degradation of Escherichia coli uncB mRNA by multiple endonucleolytic cleavages

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7 January 2021

Metal chelate affinity chromatography, a new approach to protein fractionation

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7 January 2021

Physical and functional interactions among RNase E, polynucleotide phosphorylase and the cold-shock protein, CsdA: evidence for a 'cold shock degradosome'.

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7 January 2021

Exoribonuclease R interacts with endoribonuclease E and an RNA helicase in the psychrotrophic bacterium Pseudomonas syringae Lz4W.

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7 January 2021

A DEAD-box RNA helicase in the Escherichia coli RNA degradosome

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7 January 2021

Determination of the catalytic parameters of the N-terminal half of Escherichia coli ribonuclease E and the identification of critical functional groups in RNA substrates

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7 January 2021

The Acinetobacter calcoaceticus NCIB8250 mop operon mRNA is differentially degraded, resulting in a higher level of the 3' CatA-encoding segment than of the 5' phenolhydroxylase-encoding portion

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7 January 2021

Global RNA half-life analysis in Escherichia coli reveals positional patterns of transcript degradation

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7 January 2021

Coordinated, differential expression of two genes through directed mRNA cleavage and stabilization by secondary structures

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7 January 2021

RNaseE and the other constituents of the RNA degradosome are components of the bacterial cytoskeleton

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7 January 2021

The gene specifying RNase E (rne) and a gene affecting mRNA stability (ams) are the same gene

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7 January 2021

The CafA protein required for the 5'-maturation of 16 S rRNA is a 5'-end-dependent ribonuclease that has context-dependent broad sequence specificity.

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7 January 2021

Processing enzyme ribonuclease E specifically cleaves RNA I. An inhibitor of primer formation in plasmid DNA synthesis

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7 January 2021

Involvement of RNase G in in vivo mRNA metabolism in Escherichia coli

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7 January 2021

Physiological consequences of small RNA-mediated regulation of glucose-phosphate stress

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7 January 2021

Novel phosphoramidite building blocks in synthesis and applications toward modified oligonucleotides

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7 January 2021

Escherichia coli cafA gene encodes a novel RNase, designated as RNase G, involved in processing of the 5' end of 16S rRNA

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7 January 2021

Cleavage of poly(A) tails on the 3'-end of RNA by ribonuclease E of Escherichia coli

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7 January 2021

Interaction of the restriction endonuclease EcoRV with the deoxyguanosine and deoxycytidine bases in its recognition sequence

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7 January 2021

MicroRNAs direct rapid deadenylation of mRNA

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A novel fluorogenic substrate for ribonucleases. Synthesis and enzymatic characterization

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Identifiers

DOI

1 reference

8 May 2018

1 reference

8 May 2018