(Q53112368)

https://www.ebi.ac.uk/europepmc/webservices/rest/search?query=EXT_ID:23568256%20AND%20SRC:MED&resulttype=core&format=json

28 February 2020

title

Split intein mediated ultra-rapid purification of tagless protein (SIRP) (English)

1 reference

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28 February 2020

2

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28 February 2020

author name string

Dongli Guan

series ordinal

1

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28 February 2020

Zhilei Chen

series ordinal

3

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28 February 2020

publication date

22 April 2013

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Biotechnology and Bioengineering

28 February 2020

published in

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28 February 2020

volume

110

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28 February 2020

issue

9

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28 February 2020

page(s)

2471-2481

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Segmental isotopic labeling of a central domain in a multidomain protein by protein trans-splicing using only one robust DnaE intein.

28 February 2020

cites work

1 reference

Unprecedented rates and efficiencies revealed for new natural split inteins from metagenomic sources

21 January 2018

1 reference

Intramolecular disulfide bond between catalytic cysteines in an intein precursor

21 January 2018

1 reference

Single-column purification of free recombinant proteins using a self-cleavable affinity tag derived from a protein splicing element

21 January 2018

1 reference

Utilizing the C-terminal cleavage activity of a protein splicing element to purify recombinant proteins in a single chromatographic step

21 January 2018

1 reference

Elimination of in vivo cleavage between target protein and intein in the intein-mediated protein purification systems.

21 January 2018

1 reference

Genetic definition of a protein-splicing domain: functional mini-inteins support structure predictions and a model for intein evolution

21 January 2018

1 reference

Crystal structure of PI-SceI, a homing endonuclease with protein splicing activity

21 January 2018

1 reference

Expression, purification, and characterization of human enteropeptidase catalytic subunit in Escherichia coli.

21 January 2018

1 reference

Expression and characterization of the chitin-binding domain of chitinase A1 from Bacillus circulans WL-12.

21 January 2018

1 reference

Simple protein purification through affinity adsorption on regenerated amorphous cellulose followed by intein self-cleavage

21 January 2018

1 reference

Employing mutants to study thrombin residues responsible for factor XIII activation peptide recognition: a kinetic study

21 January 2018

1 reference

Directed evolution of a thermostable phosphite dehydrogenase for NAD(P)H regeneration

21 January 2018

1 reference

Tobacco etch virus protease: mechanism of autolysis and rational design of stable mutants with wild-type catalytic proficiency

21 January 2018

1 reference

Self-cleaving fusion tags for recombinant protein production

21 January 2018

1 reference

Traceless protein splicing utilizing evolved split inteins

21 January 2018

1 reference

Split intein facilitated tag affinity purification for recombinant proteins with controllable tag removal by inducible auto-cleavage

21 January 2018

1 reference

SUMO fusions and SUMO-specific protease for efficient expression and purification of proteins.

21 January 2018

1 reference

Characterization of a self-splicing mini-intein and its conversion into autocatalytic N- and C-terminal cleavage elements: facile production of protein building blocks for protein ligation

21 January 2018

1 reference

Zinc Ion Effects on Individual Ssp DnaE Intein Splicing Steps: Regulating Pathway Progression

21 January 2018

1 reference

Mutational analysis of protein splicing, cleavage, and self-association reactions mediated by the naturally split Ssp DnaE intein

21 January 2018

1 reference

Solution structure of DnaE intein from Nostoc punctiforme: structural basis for the design of a new split intein suitable for site-specific chemical modification

21 January 2018

1 reference

Zinc is a potent inhibitor of the apoptotic protease, caspase-3. A novel target for zinc in the inhibition of apoptosis

21 January 2018

1 reference

Engineering split intein DnaE from Nostoc punctiforme for rapid protein purification.

21 January 2018

1 reference

Crystal structures of an intein from the split dnaE gene of Synechocystis sp. PCC6803 reveal the catalytic model without the penultimate histidine and the mechanism of zinc ion inhibition of protein splicing

21 January 2018

1 reference

Making the most of affinity tags

21 January 2018

1 reference

An overview of enzymatic reagents for the removal of affinity tags

21 January 2018

1 reference

Intein-mediated purification of cytotoxic endonuclease I-TevI by insertional inactivation and pH-controllable splicing

21 January 2018

1 reference

Recombinant protein purification by self-cleaving aggregation tag.

21 January 2018

1 reference

The naturally split Npu DnaE intein exhibits an extraordinarily high rate in the protein trans-splicing reaction

21 January 2018

1 reference

Cisplatin inhibits protein splicing, suggesting inteins as therapeutic targets in mycobacteria.

21 January 2018

1 reference

21 January 2018

Identifiers

DOI

10.1002/BIT.24913

1 reference

https://www.ebi.ac.uk/europepmc/webservices/rest/search?query=EXT_ID:23568256%20AND%20SRC:MED&resulttype=core&format=json

28 February 2020

1 reference

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