(Q54138034)

25 May 2018

title

Tightly controlled two-stage expression vectors employing the Flp/FRT-mediated inversion of cloned genes. (English)

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25 May 2018

2

W Szybalski

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25 May 2018

author name string

M Sektas

series ordinal

1

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25 May 2018

publication date

1 February 1998

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25 May 2018

published in

Molecular Biotechnology

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25 May 2018

volume

9

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25 May 2018

issue

1

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25 May 2018

page(s)

17-24

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25 May 2018

https://scigraph.springernature.com/pub.10.1007/bf02752694

exact match

0 references

cites work

Complete Nucleotide Sequence of the Escherichia coli Plasmid pBR322

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https://api.crossref.org/works/10.1007%2FBF02752694

A broad-host-range in vivo pop-out and amplification system for generating large quantities of 50- to 100-kb genomic fragments for direct DNA sequencing.

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Compilation of E. coli mRNA promoter sequences

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Nucleotide sequence of an RNA polymerase binding site at an early T7 promoter

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Roles of a 106-bp origin enhancer and Escherichia coli DnaA protein in replication of plasmid R6K

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Construction and evaluation of an expression vector allowing the stable expression of foreign antigens in a Salmonella typhimurium vaccine strain.

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Analysis of gene control signals by DNA fusion and cloning in Escherichia coli

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N-terminal truncated forms of the bifunctional pi initiation protein express negative activity on plasmid R6K replication.

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In vivo excision and amplification of large segments of the Escherichia coli genome

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Improved single and multicopy lac-based cloning vectors for protein and operon fusions

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Mechanisms underlying expression of Tn10 encoded tetracycline resistance

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Control of cloned gene expression by promoter inversion in vivo: construction of improved vectors with a multiple cloning site and the Ptac promoter

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Expression of genes encoding two major Theileria annulata merozoite surface antigens in Escherichia coli and a Salmonella typhimurium aroA vaccine strain

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Mutations in the trfA replication gene of the broad-host-range plasmid RK2 result in elevated plasmid copy numbers

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The FLP recombinase of the 2 micron circle DNA of yeast: interaction with its target sequences

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Site-specific recombination by Gin of bacteriophage Mu: Inversions and deletions

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Construction and characterization of new cloning vehicle. II. A multipurpose cloning system

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Construction and characterization of amplifiable multicopy DNA cloning vehicles derived from the P15A cryptic miniplasmid

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Control of cloned gene expression by promoter inversion in vivo: construction of the heat-pulse-activated att-nutL-p-att-N module

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The 2 micron circle plasmid of Saccharomyces cerevisiae

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The FLP recombinase of the yeast 2-micron plasmid: characterization of its recombination site

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Intra- and intermolecular site-specific recombination in plant cells mediated by bacteriophage P1 recombinase

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Expression of the bacteriophage P1 cin recombinase gene from its own and heterologous promoters.

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Site-specific recombinases: tools for genome engineering.

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Bending of DNA by gene-regulatory proteins: construction and use of a DNA bending vector

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From the double-helix to novel approaches to the sequencing of large genomes

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Identifiers

DOI

10.1007/BF02752694

1 reference

25 May 2018

1 reference

25 May 2018