(Q54431061)

29 May 2018

title

Preparative scale expression of membrane proteins in Escherichia coli-based continuous exchange cell-free systems. (English)

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Frank Bernhard

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Solmaz Sobhanifar

object named as

Solmaz Sobhanifar

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author name string

Daniel Schwarz

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Friederike Junge

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29 May 2018

Florian Durst

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Nadine Frölich

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Birgit Schneider

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Sina Reckel

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publication date

1 January 2007

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29 May 2018

published in

Nature Protocols

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29 May 2018

volume

2

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issue

11

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29 May 2018

page(s)

2945-2957

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https://scigraph.springernature.com/pub.10.1038/nprot.2007.426

exact match

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cites work

Protein expression systems for structural genomics and proteomics

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Preparative scale cell-free expression systems: new tools for the large scale preparation of integral membrane proteins for functional and structural studies

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Cell-free synthesis of a functional ion channel in the absence of a membrane and in the presence of detergent

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Expression of G protein coupled receptors in a cell-free translational system using detergents and thioredoxin-fusion vectors

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In vitro synthesis of fully functional EmrE, a multidrug transporter, and study of its oligomeric state

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Cell-free complements in vivo expression of the E. coli membrane proteome

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Functional expression and characterization of a bacterial light-harvesting membrane protein in Escherichia coli and cell-free synthesis systems.

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A continuous cell-free translation system capable of producing polypeptides in high yield

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A continuous cell-free protein synthesis system for coupled transcription-translation.

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Continuous-exchange protein-synthesizing systems

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An economical and highly productive cell-free protein synthesis system utilizing fructose-1,6-bisphosphate as an energy source.

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In vitro synthesis of protein in microbial systems

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A modified procedure for fast purification of T7 RNA polymerase.

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Efficient Strategy for the Rapid Backbone Assignment of Membrane Proteins

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Cell-free synthesis of 15N-labeled proteins for NMR studies

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Optimal isotope labelling for NMR protein structure determinations.

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Expression-independent consumption of substrates in cell-free expression system from Escherichia coli

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Acetyl phosphate as an energy source for bacterial cell-free translation systems.

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[41] Preparation of a cell-free protein-synthesizing system from wheat germ

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High-throughput cell-free systems for synthesis of functionally active proteins

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Prolonging cell-free protein synthesis with a novel ATP regeneration system

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Mimicking the Escherichia coli cytoplasmic environment activates long-lived and efficient cell-free protein synthesis

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Rapid production of milligram quantities of proteins in a batch cell-free protein synthesis system

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A codon window in mRNA downstream of the initiation codon where NGG codons give strongly reduced gene expression in Escherichia coli

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Production of milligram quantities of recombinant proteins from PCR-amplified DNAs in a continuous-exchange cell-free protein synthesis system.

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Cell-free synthesis of recombinant proteins from PCR-amplified genes at a comparable productivity to that of plasmid-based reactions.

31 March 2020

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Functional cell-free synthesis of a seven helix membrane protein: in situ insertion of bacteriorhodopsin into liposomes

31 March 2020

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Evaluation of detergents for the soluble expression of alpha-helical and beta-barrel-type integral membrane proteins by a preparative scale individual cell-free expression system

31 March 2020

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Cell-free expression as an emerging technique for the large scale production of integral membrane protein

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Functional analysis of cell-free-produced human endothelin B receptor reveals transmembrane segment 1 as an essential area for ET-1 binding and homodimer formation

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Efficient generation of insect-based cell-free translation extracts active in glycosylation and signal sequence processing

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31 March 2020

Identifiers

DOI

10.1038/NPROT.2007.426

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29 May 2018

1 reference

29 May 2018