(Q54481401)

30 May 2018

http://europepmc.org/abstract/MED/19455629

title

Checklist for optimization and validation of real-time PCR assays. (English)

1 reference

30 May 2018

http://europepmc.org/abstract/MED/19455629

author name string

Marijke Raymaekers

1

1 reference

https://www.ebi.ac.uk/europepmc/webservices/rest/search?query=PMCID:PMC6649018&resulttype=core&format=json

15 May 2020

Rita Smets

2

1 reference

https://www.ebi.ac.uk/europepmc/webservices/rest/search?query=PMCID:PMC6649018&resulttype=core&format=json

15 May 2020

Brigitte Maes

3

1 reference

https://www.ebi.ac.uk/europepmc/webservices/rest/search?query=PMCID:PMC6649018&resulttype=core&format=json

15 May 2020

Reinoud Cartuyvels

4

1 reference

https://www.ebi.ac.uk/europepmc/webservices/rest/search?query=PMCID:PMC6649018&resulttype=core&format=json

15 May 2020

publication date

1 January 2009

1 reference

30 May 2018

http://europepmc.org/abstract/MED/19455629

published in

Journal of Clinical Laboratory Analysis

1 reference

https://www.ebi.ac.uk/europepmc/webservices/rest/search?query=PMCID:PMC6649018&resulttype=core&format=json

15 May 2020

volume

23

1 reference

https://www.ebi.ac.uk/europepmc/webservices/rest/search?query=PMCID:PMC6649018&resulttype=core&format=json

15 May 2020

issue

3

1 reference

https://www.ebi.ac.uk/europepmc/webservices/rest/search?query=PMCID:PMC6649018&resulttype=core&format=json

15 May 2020

page(s)

145-151

1 reference

https://www.ebi.ac.uk/europepmc/webservices/rest/search?query=PMCID:PMC6649018&resulttype=core&format=json

Basic local alignment search tool

15 May 2020

cites work

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3'-minor groove binder-DNA probes increase sequence specificity at PCR extension temperatures

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Use of self-quenched, fluorogenic LUX primers for gene expression profiling

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Oligonucleotides with fluorescent dyes at opposite ends provide a quenched probe system useful for detecting PCR product and nucleic acid hybridization

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Multicolor molecular beacons for allele discrimination

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Statistical analysis of real-time PCR data

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Allelic discrimination by nick-translation PCR with fluorogenic probes

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Secondary structure in the 3' UTR of EGF and the choice of reverse transcriptases affect the detection of message diversity by RT-PCR

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Sigmoidal curve-fitting redefines quantitative real-time PCR with the prospective of developing automated high-throughput applications

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[21] Specific synthesis of DNA in vitro via a polymerase-catalyzed chain reaction

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Analysis of Relative Gene Expression Data Using Real-Time Quantitative PCR and the 2−ΔΔCT Method

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Relative quantification of mRNA: comparison of methods currently used for real-time PCR data analysis

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The real-time polymerase chain reaction

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A new quantitative method of real time reverse transcription polymerase chain reaction assay based on simulation of polymerase chain reaction kinetics

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A computer program for choosing optimal oligonucleotides for filter hybridization, sequencing and in vitro amplification of DNA

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Specific synthesis of DNA in vitro via a polymerase-catalyzed chain reaction

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12 December 2020

Identifiers

DOI

10.1002/JCLA.20307

1 reference

https://www.ebi.ac.uk/europepmc/webservices/rest/search?query=PMCID:PMC6649018&resulttype=core&format=json

15 May 2020

1 reference

https://www.ebi.ac.uk/europepmc/webservices/rest/search?query=PMCID:PMC6649018&resulttype=core&format=json

15 May 2020

1 reference