(Q54548929)

31 May 2018

title

Deletion of the NH2-terminal beta-hairpin of the ribotoxin alpha-sarcin produces a nontoxic but active ribonuclease. (English)

1 reference

31 May 2018

author

Lucía García-Ortega

1

1 reference

31 May 2018

Mercedes Oñaderra

4

1 reference

31 May 2018

Marta Bruix

7

1 reference

31 May 2018

Alvaro Martínez-del-Pozo

8

1 reference

31 May 2018

José G Gavilanes

9

1 reference

31 May 2018

José Miguel Mancheño

3

object named as

José M Mancheño

1 reference

31 May 2018

Manuel Masip

2

object named as

Manuel Masip

1 reference

31 May 2018

Ma Flor García-Mayoral

6

object named as

M Flor García-Mayoral

1 reference

31 May 2018

author name string

M Antonia Lizarbe

5

1 reference

31 May 2018

language of work or name

English

0 references

publication date

15 March 2002

1 reference

31 May 2018

Journal of Biological Chemistry

published in

1 reference

31 May 2018

volume

277

1 reference

31 May 2018

issue

21

1 reference

31 May 2018

page(s)

18632-18639

1 reference

31 May 2018

A decade of protein engineering on ribonuclease T1--atomic dissection of the enzyme-substrate interactions

cites work

1 reference

https://api.crossref.org/works/10.1074%2FJBC.M200922200

Crystal structure of the ribosomal RNA domain essential for binding elongation factors

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FJBC.M200922200

The two faces of the Escherichia coli 23 S rRNA sarcin/ricin domain: the structure at 1.11 A resolution

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FJBC.M200922200

Specific cleavage of ribosomal RNA caused by alpha sarcin

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FJBC.M200922200

Cytotoxic mechanism of the ribotoxin alpha-sarcin. Induction of cell death via apoptosis

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FJBC.M200922200

Study of the interaction between the antitumour protein alpha-sarcin and phospholipid vesicles

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FJBC.M200922200

Fusion of phospholipid vesicles produced by the anti-tumour protein alpha-sarcin

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FJBC.M200922200

Kinetic study of the aggregation and lipid mixing produced by alpha-sarcin on phosphatidylglycerol and phosphatidylserine vesicles: stopped-flow light scattering and fluorescence energy transfer measurements

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FJBC.M200922200

Membrane interaction of a beta-structure-forming synthetic peptide comprising the 116-139th sequence region of the cytotoxic protein alpha-sarcin

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FJBC.M200922200

The highly refined solution structure of the cytotoxic ribonuclease alpha-sarcin reveals the structural requirements for substrate recognition and ribonucleolytic activity

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FJBC.M200922200

Insights into specificity of cleavage and mechanism of cell entry from the crystal structure of the highly specific Aspergillus ribotoxin, restrictocin

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FJBC.M200922200

The ribonuclease T1 family

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FJBC.M200922200

The amino acid sequence of ribonuclease U2 from Ustilago sphaerogena

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FJBC.M200922200

Whole-mount in situ hybridization to mouse embryos.

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FJBC.M200922200

Crystal structure of Ustilago sphaerogena ribonuclease U2 at 1.8 A resolution

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FJBC.M200922200

Characterization of pKa values and titration shifts in the cytotoxic ribonuclease alpha-sarcin by NMR. Relationship between electrostatic interactions, structure, and catalytic function.

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FJBC.M200922200

Involvement of the amino-terminal beta-hairpin of the Aspergillus ribotoxins on the interaction with membranes and nonspecific ribonuclease activity.

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FJBC.M200922200

Overproduction and purification of biologically active native fungal alpha-sarcin in Escherichia coli.

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FJBC.M200922200

Substitution of histidine-137 by glutamine abolishes the catalytic activity of the ribosome-inactivating protein alpha-sarcin

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FJBC.M200922200

Rapid and efficient site-specific mutagenesis without phenotypic selection

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FJBC.M200922200

A two-dimensional nuclear Overhauser enhancement (2D NOE) experiment for the elucidation of complete proton-proton cross-relaxation networks in biological macromolecules

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FJBC.M200922200

Gradient-tailored excitation for single-quantum NMR spectroscopy of aqueous solutions

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FJBC.M200922200

Solution structure and dynamics of ras p21.GDP determined by heteronuclear three- and four-dimensional NMR spectroscopy

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FJBC.M200922200

1H and 15N nuclear magnetic resonance assignment and secondary structure of the cytotoxic ribonuclease alpha-Sarcin

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FJBC.M200922200

Translocation of alpha-sarcin across the lipid bilayer of asolectin vesicles.

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FJBC.M200922200

Convex constraint analysis: a natural deconvolution of circular dichroism curves of proteins.

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FJBC.M200922200

21 January 2018

Protein stability curves

1 reference

https://api.crossref.org/works/10.1074%2FJBC.M200922200

Fungal ribotoxins: a family of naturally engineered targeted toxins?

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FJBC.M200922200

Immunotoxins: a clinical review of their use in the treatment of malignancies

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FJBC.M200922200

Biochemical, cytotoxic and pharmacokinetic properties of an immunotoxin composed of a mouse monoclonal antibody Fib75 and the ribosome-inactivating protein alpha-sarcin from Aspergillus giganteus

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FJBC.M200922200

Immunodiagnosis of aspergillosis

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FJBC.M200922200

The subsites structure of bovine pancreatic ribonuclease A accounts for the abnormal kinetic behavior with cytidine 2',3'-cyclic phosphate

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FJBC.M200922200

Methyl-CpG-binding proteins. Targeting specific gene repression.

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FJBC.M200922200

Crystal structures of restrictocin-inhibitor complexes with implications for RNA recognition and base flipping

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FJBC.M200922200

MOLMOL: a program for display and analysis of macromolecular structures

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FJBC.M200922200

Reconsidering the energetics of ribonuclease catalysed RNA hydrolysis

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FJBC.M200922200

Mitogillin and related fungal ribotoxins

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FJBC.M200922200

Kinetic study of the cytotoxic effect of alpha-sarcin, a ribosome inactivating protein from Aspergillus giganteus, on tumour cell lines: protein biosynthesis inhibition and cell binding

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FJBC.M200922200

Oligomerization of the cytotoxin α-sarcin associated with phospholipid membranes

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FJBC.M200922200

Predictive study of the conformation of the cytotoxic protein α-sarcin: a structural model to explain α-sarcin-membrane interaction

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FJBC.M200922200

Revised sequence of ribonuclease U2 in the substrate-binding region

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FJBC.M200922200

The cytotoxin α-sarcin behaves as a cyclizing ribonuclease

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FJBC.M200922200

Role of histidine-50, glutamic acid-96, and histidine-137 in the ribonucleolytic mechanism of the ribotoxin ?-sarcin

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FJBC.M200922200

Characterization of enzymatic specificity of a ribonuclease from Ustilago sphaerogena

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FJBC.M200922200

Increase of Solubility of Foreign Proteins in Escherichia coli by Coproduction of the Bacterial Thioredoxin

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FJBC.M200922200

The solubility of the ribotoxin alpha-sarcin, produced as a recombinant protein in Escherichia coli, is increased in the presence of thioredoxin

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FJBC.M200922200

Molecular dissection of mitogillin reveals that the fungal ribotoxins are a family of natural genetically engineered ribonucleases

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FJBC.M200922200

Activity staining of nucleolytic enzymes after sodium dodecyl sulfate-polyacrylamide gel electrophoresis: Use of aqueous isopropanol to remove detergent from gels

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FJBC.M200922200

A peptide of nine amino acid residues from alpha-sarcin cytotoxin is a membrane-perturbing structure

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FJBC.M200922200

Assignment of the contribution of the tryptophan residues to the spectroscopic and functional properties of the ribotoxin alpha-sarcin

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FJBC.M200922200

Energetics of ribonuclease A catalysis. 2. Nonenzymatic hydrolysis of cytidine cyclic 2',3'-phosphate

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FJBC.M200922200

21 January 2018

Identifiers

DOI

10.1074/JBC.M200922200

1 reference

31 May 2018

1 reference

31 May 2018