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Combinatorial recombination of gene fragments to construct a library of chimeras

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Combinatorial recombination of gene fragments to construct a library of chimeras (English)

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Frances H Arnold

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Mary F Farrow

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1

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English

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August 2010

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Chapter 26

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Unit 26.2

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On the conservative nature of intragenic recombination

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Site-directed protein recombination as a shortest-path problem

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Hybrid native partitioning of interactions among nonconserved residues in chimeric proteins.

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A family of thermostable fungal cellulases created by structure-guided recombination

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General method for sequence-independent site-directed chimeragenesis

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A diverse family of thermostable cytochrome P450s created by recombination of stabilizing fragments

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Structure-based chimeric enzymes as an alternative to directed enzyme evolution: phytase as a test case.

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Analysis of shuffled gene libraries

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Creating multiple-crossover DNA libraries independent of sequence identity

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Analysis of shuffled libraries by oligonucleotide probe hybridization

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Library analysis of SCHEMA-guided protein recombination

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Pig liver carnitine palmitoyltransferase. Chimera studies show that both the N- and C-terminal regions of the enzyme are important for the unusual high malonyl-CoA sensitivity

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Incremental truncation as a strategy in the engineering of novel biocatalysts

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Functional evolution and structural conservation in chimeric cytochromes p450: calibrating a structure-guided approach

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Chimeric Enzymes of Cytochrome P450 Oxidoreductase and Neuronal Nitric-oxide Synthase Reductase Domain Reveal Structural and Functional Differences

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FamClash: a method for ranking the activity of engineered enzymes.

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Design of combinatorial protein libraries of optimal size

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Libraries of hybrid proteins from distantly related sequences

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SCHEMA-guided protein recombination

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A combinatorial approach to hybrid enzymes independent of DNA homology

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Phosphatases and kinases

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Chimeras of X+, K+-ATPases. The M1-M6 region of Na+, K+-ATPase is required for Na+-activated ATPase activity, whereas the M7-M10 region of H+, K+-ATPase is involved in K+ de-occlusion

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10.1002/0471140864.PS2602S61

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20814931

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(Q56896919)

Combinatorial recombination of gene fragments to construct a library of chimeras

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