(Q56900938)

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Molecular characterization of the poly(3-hydroxybutyrate) (PHB) synthase from Ralstonia eutropha: in vitro evolution, site-specific mutagenesis and development of a PHB synthase protein model

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Europe PubMed Central

https://www.ebi.ac.uk/europepmc/webservices/rest/search?query=EXT_ID:11825620%20AND%20SRC:MED&resulttype=core&format=json

6 December 2019

Molecular characterization of the poly(3-hydroxybutyrate) (PHB) synthase from Ralstonia eutropha: in vitro evolution, site-specific mutagenesis and development of a PHB synthase protein model (English)

1 reference

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6 December 2019

protein evolution

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polyhydroxybutyrate

1 reference

based on heuristic

inferred from title

Ralstonia eutropha

object named as

Ralstonia eutropha

1 reference

based on heuristic

inferred from title

1

1 reference

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6 December 2019

Alexander Steinbüchel

5

object named as

Alexander Steinbüchel

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6 December 2019

author name string

Regina V Antonio

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6 December 2019

Patricia Spiekermann

3

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6 December 2019

Amro A Amara

4

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6 December 2019

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1 January 2002

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6 December 2019

Biochimica et Biophysica Acta

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6 December 2019

1594

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6 December 2019

1

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6 December 2019

178-190

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6 December 2019

Identification of a new class of biopolymer: bacterial synthesis of a sulfur-containing polymer with thioester linkages

1 reference

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7 January 2021

based on heuristic

inferred from DOI database lookup

Cloning and nucleotide sequences of genes relevant for biosynthesis of poly(3-hydroxybutyric acid) in Chromatium vinosum strain D

1 reference

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7 January 2021

based on heuristic

inferred from DOI database lookup

Analysis of the Thiocapsa pfennigii polyhydroxyalkanoate synthase: subcloning, molecular characterization and generation of hybrid synthases with the corresponding Chromatium vinosum enzyme

1 reference

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based on heuristic

inferred from DOI database lookup

Overexpression and purification of the soluble polyhydroxyalkanoate synthase from Alcaligenes eutrophus: evidence for a required posttranslational modification for catalytic activity.

1 reference

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7 January 2021

based on heuristic

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Enzyme-catalyzed synthesis of poly[(R)-(-)-3-hydroxybutyrate]: formation of macroscopic granules in vitro

1 reference

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7 January 2021

based on heuristic

inferred from DOI database lookup

Purification and characterization of the poly(hydroxyalkanoic acid) synthase from Chromatium vinosum and localization of the enzyme at the surface of poly(hydroxyalkanoic acid) granules.

1 reference

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7 January 2021

based on heuristic

inferred from DOI database lookup

In vitro biosynthesis of poly(3-hydroxybutyric acid) by using purified poly(hydroxyalkanoic acid) synthase of Chromatium vinosum

1 reference

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7 January 2021

based on heuristic

inferred from DOI database lookup

Cloning and molecular analysis of the poly(3-hydroxyalkanoic acid) gene locus of Pseudomonas aeruginosa PAO1

1 reference

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7 January 2021

based on heuristic

inferred from DOI database lookup

Functional expression of the PHA synthase gene phaC1 from Pseudomonas aeruginosa in Escherichia coli results in poly(3-hydroxyalkanoate) synthesis.

1 reference

https://api.crossref.org/works/10.1016%2FS0167-4838%2801%2900299-0

7 January 2021

based on heuristic

inferred from DOI database lookup

Synthesis of poly(3-hydroxyalkanoates) in Escherichia coli expressing the PHA synthase gene phaC2 from Pseudomonas aeruginosa: comparison of PhaC1 and PhaC2

1 reference

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7 January 2021

based on heuristic

inferred from DOI database lookup

A new metabolic link between fatty acid de novo synthesis and polyhydroxyalkanoic acid synthesis. The PHAG gene from Pseudomonas putida KT2440 encodes a 3-hydroxyacyl-acyl carrier protein-coenzyme a transferase.

1 reference

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7 January 2021

based on heuristic

inferred from DOI database lookup

PhaG-mediated synthesis of Poly(3-hydroxyalkanoates) consisting of medium-chain-length constituents from nonrelated carbon sources in recombinant Pseudomonas fragi.

1 reference

https://api.crossref.org/works/10.1016%2FS0167-4838%2801%2900299-0

7 January 2021

based on heuristic

inferred from DOI database lookup

Role of fatty acid de novo biosynthesis in polyhydroxyalkanoic acid (PHA) and rhamnolipid synthesis by pseudomonads: establishment of the transacylase (PhaG)-mediated pathway for PHA biosynthesis in Escherichia coli

1 reference

https://api.crossref.org/works/10.1016%2FS0167-4838%2801%2900299-0

7 January 2021

based on heuristic

inferred from DOI database lookup

The Pseudomonas aeruginosa phaG gene product is involved in the synthesis of polyhydroxyalkanoic acid consisting of medium-chain-length constituents from non-related carbon sources

1 reference

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7 January 2021

based on heuristic

inferred from DOI database lookup

Homologous functional expression of cryptic phaG from Pseudomonas oleovorans establishes the transacylase-mediated polyhydroxyalkanoate biosynthetic pathway.

1 reference

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7 January 2021

based on heuristic

inferred from DOI database lookup

In vitro synthesis of poly(3-hydroxydecanoate): purification and enzymatic characterization of type II polyhydroxyalkanoate synthases PhaC1 and PhaC2 from Pseudomonas aeruginosa

1 reference

https://api.crossref.org/works/10.1016%2FS0167-4838%2801%2900299-0

7 January 2021

based on heuristic

inferred from DOI database lookup

Lipases provide a new mechanistic model for polyhydroxybutyrate (PHB) synthases: characterization of the functional residues in Chromatium vinosum PHB synthase.

1 reference

https://api.crossref.org/works/10.1016%2FS0167-4838%2801%2900299-0

7 January 2021

based on heuristic

inferred from DOI database lookup

Hidden Markov models for detecting remote protein homologies

1 reference

https://api.crossref.org/works/10.1016%2FS0167-4838%2801%2900299-0

7 January 2021

based on heuristic

inferred from DOI database lookup

Molecular analysis of the Alcaligenes eutrophus poly(3-hydroxybutyrate) biosynthetic operon: identification of the N terminus of poly(3-hydroxybutyrate) synthase and identification of the promoter

1 reference

https://api.crossref.org/works/10.1016%2FS0167-4838%2801%2900299-0

7 January 2021

based on heuristic

inferred from DOI database lookup

Analysis of 4-phosphopantetheinylation of polyhydroxybutyrate synthase from Ralstonia eutropha: generation of beta-alanine auxotrophic Tn5 mutants and cloning of the panD gene region

1 reference

https://api.crossref.org/works/10.1016%2FS0167-4838%2801%2900299-0

7 January 2021

based on heuristic

inferred from DOI database lookup

Mechanistic studies on class I polyhydroxybutyrate (PHB) synthase from Ralstonia eutropha: class I and III synthases share a similar catalytic mechanism.

1 reference

https://api.crossref.org/works/10.1016%2FS0167-4838%2801%2900299-0

7 January 2021

based on heuristic

inferred from DOI database lookup

Analysis of mutational effects of a polyhydroxybutyrate (PHB) polymerase on bacterial PHB accumulation using an in vivo assay system

1 reference

https://api.crossref.org/works/10.1016%2FS0167-4838%2801%2900299-0

7 January 2021

based on heuristic

inferred from DOI database lookup

PHA synthase from chromatium vinosum: cysteine 149 is involved in covalent catalysis

1 reference

https://api.crossref.org/works/10.1016%2FS0167-4838%2801%2900299-0

7 January 2021

based on heuristic

inferred from DOI database lookup

Biochemical and genetic analysis of PHA synthases and other proteins required for PHA synthesis.

1 reference

https://api.crossref.org/works/10.1016%2FS0167-4838%2801%2900299-0

7 January 2021

based on heuristic

inferred from DOI database lookup

Primary sequence of the Escherichia coli fadBA operon, encoding the fatty acid-oxidizing multienzyme complex, indicates a high degree of homology to eucaryotic enzymes

1 reference

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based on heuristic

inferred from DOI database lookup

A sensitive, viable-colony staining method using Nile red for direct screening of bacteria that accumulate polyhydroxyalkanoic acids and other lipid storage compounds

1 reference

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based on heuristic

inferred from DOI database lookup

Membrane topology of the outer membrane protein OprH from Pseudomonas aeruginosa: PCR-mediated site-directed insertion and deletion mutagenesis

1 reference

https://api.crossref.org/works/10.1016%2FS0167-4838%2801%2900299-0

7 January 2021

based on heuristic

inferred from DOI database lookup

Rapid evolution of a protein in vitro by DNA shuffling

1 reference

https://api.crossref.org/works/10.1016%2FS0167-4838%2801%2900299-0

7 January 2021

based on heuristic

inferred from DOI database lookup

Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications

1 reference

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7 January 2021

based on heuristic

inferred from DOI database lookup

Analysis of in vivo substrate specificity of the PHA synthase from Ralstonia eutropha: formation of novel copolyesters in recombinant Escherichia coli

1 reference

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7 January 2021

based on heuristic

inferred from DOI database lookup

Pseudomonas oleovorans as a Source of Poly(beta-Hydroxyalkanoates) for Potential Applications as Biodegradable Polyesters.

1 reference

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based on heuristic

inferred from DOI database lookup

PROCHECK: a program to check the stereochemical quality of protein structures

1 reference

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based on heuristic

inferred from DOI database lookup

Assessment of protein models with three-dimensional profiles

1 reference

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based on heuristic

inferred from DOI database lookup

Protein fold recognition using sequence-derived predictions

1 reference

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based on heuristic

inferred from DOI database lookup

The crystal structure of triacylglycerol lipase from Pseudomonas glumae reveals a partially redundant catalytic aspartate

1 reference

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based on heuristic

inferred from DOI database lookup

The open conformation of a Pseudomonas lipase

1 reference

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based on heuristic

inferred from DOI database lookup

Identifiers

10.1016/S0167-4838(01)00299-0

1 reference

Europe PubMed Central

https://www.ebi.ac.uk/europepmc/webservices/rest/search?query=EXT_ID:11825620%20AND%20SRC:MED&resulttype=core&format=json

6 December 2019

1 reference

Europe PubMed Central

https://www.ebi.ac.uk/europepmc/webservices/rest/search?query=EXT_ID:11825620%20AND%20SRC:MED&resulttype=core&format=json

6 December 2019

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