(Q30777925)

26 June 2017

title

Generation of a broad esterolytic subtilisin using combined molecular evolution and periplasmic expression (English)

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26 June 2017

Sroga GE

1

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26 June 2017

Dordick JS

series ordinal

2

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26 June 2017

publication date

1 November 2001

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Protein Engineering Design and Selection

26 June 2017

published in

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26 June 2017

volume

14

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26 June 2017

issue

11

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26 June 2017

page(s)

929-937

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Probing the importance of second sphere residues in an esterolytic antibody by phage display

26 June 2017

cites work

1 reference

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21 January 2018

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Selection for improved subtiligases by phage display

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Construction and characterization of Escherichia coli strains deficient in multiple secreted proteases: protease III degrades high-molecular-weight substrates in vivo

21 January 2018

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Chimeric proteins can exceed the sum of their parts: implications for evolution and protein design

21 January 2018

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Analysis of large libraries of protein mutants using flow cytometry

21 January 2018

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Directed evolution of an esterase from Pseudomonas fluorescens. Random mutagenesis by error-prone PCR or a mutator strain and identification of mutants showing enhanced enantioselectivity by a resorufin-based fluorescence assay.

21 January 2018

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New enzyme lineages by subdomain shuffling

21 January 2018

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Bacterial biocatalysts: molecular biology, three-dimensional structures, and biotechnological applications of lipases

21 January 2018

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Laboratory evolution of peroxide-mediated cytochrome P450 hydroxylation

21 January 2018

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Elucidating structure-mechanism relationships in lipases: prospects for predicting and engineering catalytic properties

21 January 2018

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Effect of organic solvents on enantioselectivity of protease catalysis.

21 January 2018

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Improving enzymes by using them in organic solvents

21 January 2018

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Serine Proteases: Structure and Mechanism of Catalysis

21 January 2018

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Directed evolution of enzyme catalysts

21 January 2018

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DNA shuffling of subgenomic sequences of subtilisin

21 January 2018

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Hybrid enzymes: manipulating enzyme design

21 January 2018

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Structural analysis of affinity matured antibodies and laboratory-evolved enzymes

21 January 2018

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Redesigning the substrate specificity of an enzyme by cumulative effects of the mutations of non-active site residues

21 January 2018

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Industrial biocatalysis today and tomorrow

21 January 2018

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Subtilases: the superfamily of subtilisin-like serine proteases

21 January 2018

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DNA shuffling by random fragmentation and reassembly: in vitro recombination for molecular evolution

21 January 2018

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21 January 2018

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Designing substrate specificity by protein engineering of electrostatic interactions

21 January 2018

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Protein folding in the periplasm of Escherichia coli

21 January 2018

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Directed evolution of subtilisin E in Bacillus subtilis to enhance total activity in aqueous dimethylformamide

21 January 2018

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Directed evolution converts subtilisin E into a functional equivalent of thermitase

21 January 2018

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Molecular evolution by staggered extension process (StEP) in vitro recombination

21 January 2018

1 reference

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Pro-sequence of subtilisin can guide the refolding of denatured subtilisin in an intermolecular process.

21 January 2018

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Directed evolution of an enantioselective lipase

21 January 2018

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10.1093/PROTEIN/14.11.929

21 January 2018

Identifiers

DOI

1 reference

26 June 2017

1 reference