(Q31016777)

English

Enhanced crossover SCRATCHY: construction and high-throughput screening of a combinatorial library containing multiple non-homologous crossovers.

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scholarly article

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Europe PubMed Central

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20 December 2019

Enhanced crossover SCRATCHY: construction and high-throughput screening of a combinatorial library containing multiple non-homologous crossovers (English)

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20 December 2019

high-throughput screening

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Yasuaki Kawarasaki

1

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20 December 2019

author name string

Karl E Griswold

2

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20 December 2019

James D Stevenson

3

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20 December 2019

Tzvia Selzer

4

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20 December 2019

Stephen J Benkovic

5

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20 December 2019

Brent L Iverson

6

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20 December 2019

George Georgiou

7

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20 December 2019

language of work or name

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publication date

1 November 2003

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20 December 2019

Nucleic Acids Research

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20 December 2019

31

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20 December 2019

e126

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20 December 2019

21

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20 December 2019

Screening for recombinant glutathione transferases active with monochlorobimane

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=275483

21 June 2018

An ensemble of theta class glutathione transferases with novel catalytic properties generated by stochastic recombination of fragments of two mammalian enzymes

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=275483

21 June 2018

Generating segmental mutations in haloalkane dehalogenase: a novel part in the directed evolution toolbox

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=275483

21 June 2018

Analysis of shuffled gene libraries

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=275483

21 June 2018

Novel enzyme activities and functional plasticity revealed by recombining highly homologous enzymes

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=275483

21 June 2018

Creating multiple-crossover DNA libraries independent of sequence identity

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=275483

21 June 2018

Libraries of hybrid proteins from distantly related sequences

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=275483

21 June 2018

Predicting crossover generation in DNA shuffling.

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=275483

21 June 2018

Rapid generation of incremental truncation libraries for protein engineering using alpha-phosphothioate nucleotides.

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=275483

21 June 2018

Mammalian class theta GST and differential susceptibility to carcinogens: a review

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=275483

21 June 2018

Homology-independent protein engineering

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=275483

21 June 2018

A combinatorial approach to hybrid enzymes independent of DNA homology

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=275483

21 June 2018

Directed evolution of thymidine kinase for AZT phosphorylation using DNA family shuffling

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=275483

21 June 2018

Enhanced degradation of polychlorinated biphenyls by directed evolution of biphenyl dioxygenase.

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=275483

21 June 2018

DNA shuffling of a family of genes from diverse species accelerates directed evolution

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=275483

21 June 2018

Kinetic characterization of recombinant human glutathione transferase T1-1, a polymorphic detoxication enzyme

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=275483

21 June 2018

Structure, catalytic mechanism, and evolution of the glutathione transferases

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=275483

21 June 2018

Homology model for the human GSTT2 Theta class glutathione transferase

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=275483

21 June 2018

Heterologous expression, purification and characterization of rat class theta glutathione transferase T2-2

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=275483

21 June 2018

DNA shuffling by random fragmentation and reassembly: in vitro recombination for molecular evolution

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=275483

21 June 2018

Novel family shuffling methods for the in vitro evolution of enzymes

1 reference

https://pubmed.ncbi.nlm.nih.gov/14576326

12 December 2020

based on heuristic

inferred from PubMed ID database lookup

Identifiers

10.1093/NAR/GNG126

1 reference

Europe PubMed Central

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20 December 2019

1 reference

Europe PubMed Central

https://www.ebi.ac.uk/europepmc/webservices/rest/search?query=EXT_ID:14576326%20AND%20SRC:MED&resulttype=core&format=json

20 December 2019

1 reference

Europe PubMed Central

https://www.ebi.ac.uk/europepmc/webservices/rest/search?query=EXT_ID:14576326%20AND%20SRC:MED&resulttype=core&format=json

20 December 2019

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