(Q33298809)

25 July 2017

Design and implementation of high-throughput RNAi screens in cultured Drosophila cells (English)

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25 July 2017

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Matthew Booker

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Norbert Perrimon

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Norbert Perrimon

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author name string

Nadire Ramadan

1

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25 July 2017

Ian Flockhart

2

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25 July 2017

Bernard Mathey-Prevot

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25 July 2017

language of work or name

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1 January 2007

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25 July 2017

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9

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25 July 2017

2245-2264

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https://scigraph.springernature.com/pub.10.1038/nprot.2007.250

25 July 2017

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Use of double-stranded RNA interference in Drosophila cell lines to dissect signal transduction pathways.

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An RNA-directed nuclease mediates post-transcriptional gene silencing in Drosophila cells

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Identification of Hedgehog pathway components by RNAi in Drosophila cultured cells.

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A functional genomic analysis of cell morphology using RNA interference

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Functional dissection of an innate immune response by a genome-wide RNAi screen

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Genome-wide RNAi analysis of growth and viability in Drosophila cells

21 January 2018

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Functional genomic analysis of phagocytosis and identification of a Drosophila receptor for E. coli

21 January 2018

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A mutation in Orai1 causes immune deficiency by abrogating CRAC channel function

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CRACM1 is a plasma membrane protein essential for store-operated Ca2+ entry

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Genome-wide RNAi screen of Ca(2+) influx identifies genes that regulate Ca(2+) release-activated Ca(2+) channel activity

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Applications of high-throughput RNA interference screens to problems in cell and developmental biology

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Use of dsRNA-mediated genetic interference to demonstrate that frizzled and frizzled 2 act in the wingless pathway.

21 January 2018

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Heritable gene silencing in Drosophila using double-stranded RNA.

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A functional genomic screen for cardiogenic genes using RNA interference in developing Drosophila embryos

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RNA interference screen to identify genes required for Drosophila embryonic nervous system development.

21 January 2018

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Prevalence of off-target effects in Drosophila RNA interference screens

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Evidence of off-target effects associated with long dsRNAs in Drosophila melanogaster cell-based assays

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Minimizing the risk of reporting false positives in large-scale RNAi screens

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Matter arising: off-targets and genome-scale RNAi screens in Drosophila

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Functional analysis of immune response genes in Drosophila identifies JNK pathway as a regulator of antimicrobial peptide gene expression in S2 cells

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Identification of pathways regulating cell size and cell-cycle progression by RNAi

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Analysis of cell-based RNAi screens.

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Robust statistical methods for hit selection in RNA interference high-throughput screening experiments

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Cellular phenotyping by RNAi.

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RNA interference pinpoints regulators of cell size and the cell cycle

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High-throughput RNAi screening in cultured cells: a user's guide.

21 January 2018

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An integrated gene annotation and transcriptional profiling approach towards the full gene content of the Drosophila genome

21 January 2018

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Statistical practice in high-throughput screening data analysis

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An efficient method for the detection and elimination of systematic error in high-throughput screening.

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21 January 2018

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A database and tool, IM Browser, for exploring and integrating emerging gene and protein interaction data for Drosophila

21 January 2018

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Functional genomic analysis of the Wnt-wingless signaling pathway

21 January 2018

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Identification and characterization of a novel line of Drosophila Schneider S2 cells that respond to wingless signaling

21 January 2018

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Newly established cell lines from Drosophila larval CNS express neural specific characteristics

21 January 2018

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Cell lines from imaginal discs of Drosophila melanogaster

21 January 2018

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Essential genes are more evolutionarily conserved than are nonessential genes in bacteria

21 January 2018

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Evidence for endogenous reovirus-like particles in a tissue culture cell line from Drosophila melanogaster

31 March 2020

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31 March 2020

Identifiers

DOI

10.1038/NPROT.2007.250

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Dimensions Publication ID

25 July 2017

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