(Q34323205)

1 August 2017

title

Synthetic antibodies: concepts, potential and practical considerations (English)

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1 August 2017

1 reference

S Miersch

1

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1 August 2017

S S Sidhu

series ordinal

2

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1 August 2017

publication date

27 June 2012

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1 August 2017

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1 August 2017

volume

57

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1 August 2017

issue

4

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1 August 2017

page(s)

486-498

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Development trends for human monoclonal antibody therapeutics

1 August 2017

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High-avidity human IgG kappa monoclonal antibodies from a novel strain of minilocus transgenic mice

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7 January 2021

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Antibody humanization using monovalent phage display

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Phage display of a catalytic antibody to optimize affinity for transition-state analog binding

7 January 2021

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7 January 2021

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7 January 2021

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Isolation of high affinity human antibodies directly from large synthetic repertoires.

7 January 2021

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7 January 2021

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Eliminating helper phage from phage display

7 January 2021

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Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors

7 January 2021

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7 January 2021

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A new helper phage and phagemid vector system improves viral display of antibody Fab fragments and avoids propagation of insert-less virions

7 January 2021

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Genetic and epigenetic control of V gene rearrangement frequency.

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Utilization of Ig heavy chain variable, diversity, and joining gene segments in children with B-lineage acute lymphoblastic leukemia: implications for the mechanisms of VDJ recombination and for pathogenesis

7 January 2021

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A large array of human monoclonal antibodies to type 1 human immunodeficiency virus from combinatorial libraries of asymptomatic seropositive individuals

7 January 2021

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Human antibodies with sub-nanomolar affinities isolated from a large non-immunized phage display library

7 January 2021

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Modelling the human immune response: performance of a 1011 human antibody repertoire against a broad panel of therapeutically relevant antigens

7 January 2021

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Synthetic human antibodies

7 January 2021

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The importance of being tyrosine: lessons in molecular recognition from minimalist synthetic binding proteins

7 January 2021

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Exploring and designing protein function with restricted diversity.

7 January 2021

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Selection and application of human single chain Fv antibody fragments from a semi-synthetic phage antibody display library with designed CDR3 regions

7 January 2021

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By-passing immunisation. Human antibodies from synthetic repertoires of germline VH gene segments rearranged in vitro

7 January 2021

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An antibody single-domain phage display library of a native heavy chain variable region: isolation of functional single-domain VH molecules with a unique interface.

7 January 2021

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Selection of human anti-hapten antibodies from semisynthetic libraries

7 January 2021

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Trinucleotide phosphoramidites: ideal reagents for the synthesis of mixed oligonucleotides for random mutagenesis

7 January 2021

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The synthesis of blocked triplet-phosphoramidites and their use in mutagenesis

7 January 2021

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The human combinatorial antibody library HuCAL GOLD combines diversification of all six CDRs according to the natural immune system with a novel display method for efficient selection of high-affinity antibodies.

7 January 2021

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Function blocking antibodies to neuropilin-1 generated from a designed human synthetic antibody phage library

7 January 2021

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Construction of a semisynthetic antibody library using trinucleotide oligos

7 January 2021

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Kabat Database and its applications: 30 years after the first variability plot

7 January 2021

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Synthetic antibodies designed on natural sequence landscapes

7 January 2021

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Stability and CDR composition biases enrich binder functionality landscapes

7 January 2021

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Accelerating phage-display library selection by reversible and site-specific biotinylation

7 January 2021

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Synthetic antibodies from a four-amino-acid code: a dominant role for tyrosine in antigen recognition

7 January 2021

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Molecular recognition by a binary code

7 January 2021

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Recombining germline-derived CDR sequences for creating diverse single-framework antibody libraries

7 January 2021

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Successful application of the dual-vector system II in creating a reliable phage-displayed combinatorial Fab library

7 January 2021

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Generation of high-affinity human antibodies by combining donor-derived and synthetic complementarity-determining-region diversity.

7 January 2021

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Rapid and efficient site-specific mutagenesis without phenotypic selection

7 January 2021

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High-efficiency transformation of bacterial cells by electroporation

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DNA transfection of Escherichia coli by electroporation

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High efficiency transformation of E. coli by high voltage electroporation

7 January 2021

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The physical and functional behavior of capture antibodies adsorbed on polystyrene

7 January 2021

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Protein production and purification

7 January 2021

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PSI:Biology-materials repository: a biologist's resource for protein expression plasmids

7 January 2021

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Oligohis-tags: mechanisms of binding to Ni2+-NTA surfaces

7 January 2021

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Double-hexahistidine tag with high-affinity binding for protein immobilization, purification, and detection on ni-nitrilotriacetic acid surfaces.

7 January 2021

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Optimizing antibody immobilization strategies for the construction of protein microarrays

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Strong and oriented immobilization of single domain antibodies from crude bacterial lysates for high-throughput compatible cost-effective antibody array generation

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1 reference

https://api.crossref.org/works/10.1016%2FJ.YMETH.2012.06.012

Introduction to avidin-biotin technology

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FJ.YMETH.2012.06.012

Optimization of biotin labeling of antibodies using mouse IgG and goat anti-mouse IgG-conjugated fluorescent beads and their application as capture probes on protein chip

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FJ.YMETH.2012.06.012

Comparison of affinity tags for protein purification

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FJ.YMETH.2012.06.012

HaloTag: a novel protein labeling technology for cell imaging and protein analysis

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FJ.YMETH.2012.06.012

High-affinity adaptors for switchable recognition of histidine-tagged proteins

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FJ.YMETH.2012.06.012

Noncovalent, site-specific biotinylation of histidine-tagged proteins

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FJ.YMETH.2012.06.012

Inhibition of human leukemia in an animal model with human antibodies directed against vascular endothelial growth factor receptor 2. Correlation between antibody affinity and biological activity

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FJ.YMETH.2012.06.012

Antigen specificity and tumour targeting efficiency of a human carcinoembryonic antigen-specific scFv and affinity-matured derivatives

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FJ.YMETH.2012.06.012

Generating improved single-chain Fv molecules for tumor targeting

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FJ.YMETH.2012.06.012

Tailoring in vitro selection for a picomolar affinity human antibody directed against vascular endothelial growth factor receptor 2 for enhanced neutralizing activity

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FJ.YMETH.2012.06.012

Crystal structure of an in vitro affinity- and specificity-matured anti-testosterone Fab in complex with testosterone. Improved affinity results from small structural changes within the variable domains

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FJ.YMETH.2012.06.012

Selection of phage antibodies by binding affinity

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FJ.YMETH.2012.06.012

Computational analysis of off-rate selection experiments to optimize affinity maturation by directed evolution

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FJ.YMETH.2012.06.012

Combinatorial libraries against libraries for selecting neoepitope activation-specific antibodies

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FJ.YMETH.2012.06.012

Selection for improved protein stability by phage display.

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FJ.YMETH.2012.06.012

Cross-species vascular endothelial growth factor (VEGF)-blocking antibodies completely inhibit the growth of human tumor xenografts and measure the contribution of stromal VEGF.

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FJ.YMETH.2012.06.012

Synthetic anti-BR3 antibodies that mimic BAFF binding and target both human and murine B cells

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FJ.YMETH.2012.06.012

Structure-function studies of two synthetic anti-vascular endothelial growth factor Fabs and comparison with the Avastin Fab.

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FJ.YMETH.2012.06.012

A two-in-one antibody against HER3 and EGFR has superior inhibitory activity compared with monospecific antibodies

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FJ.YMETH.2012.06.012

Variants of the antibody herceptin that interact with HER2 and VEGF at the antigen binding site

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FJ.YMETH.2012.06.012

Isolation of a novel neutralizing antibody fragment against human vascular endothelial growth factor from a phage-displayed human antibody repertoire using an epitope disturbing strategy

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FJ.YMETH.2012.06.012

A reliable method for random mutagenesis: the generation of mutant libraries using spiked oligodeoxyribonucleotide primers

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FJ.YMETH.2012.06.012

Comprehensive functional maps of the antigen-binding site of an anti-ErbB2 antibody obtained with shotgun scanning mutagenesis

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FJ.YMETH.2012.06.012

Alternative views of functional protein binding epitopes obtained by combinatorial shotgun scanning mutagenesis

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FJ.YMETH.2012.06.012

Antibodies to human fetal erythroid cells from a nonimmune phage antibody library

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FJ.YMETH.2012.06.012

Isolation and tissue profiles of a large panel of phage antibodies binding to the human adipocyte cell surface

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FJ.YMETH.2012.06.012

Internalizing cancer antibodies from phage libraries selected on tumor cells and yeast-displayed tumor antigens

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FJ.YMETH.2012.06.012

Development of anti-tumor blood vessel antibodies by phage display method

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FJ.YMETH.2012.06.012

Highly efficient selection of phage antibodies mediated by display of antigen as Lpp-OmpA' fusions on live bacteria

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FJ.YMETH.2012.06.012

Isolation of anti-CD22 Fv with high affinity by Fv display on human cells

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FJ.YMETH.2012.06.012

Selection of cell binding and internalizing epidermal growth factor receptor antibodies from a phage display library

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FJ.YMETH.2012.06.012

Use of proteoliposomes to generate phage antibodies against native AMPA receptor

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FJ.YMETH.2012.06.012

Phage-display selection on tumor histological specimens with laser capture microdissection

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FJ.YMETH.2012.06.012

Selection of antibodies against a single rare cell present in a heterogeneous population using phage display.

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FJ.YMETH.2012.06.012

Microselection--affinity selecting antibodies against a single rare cell in a heterogeneous population.

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FJ.YMETH.2012.06.012

Directed selection of MIP-1 alpha neutralizing CCR5 antibodies from a phage display human antibody library.

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FJ.YMETH.2012.06.012

Novel strategy for the selection of human recombinant Fab fragments to membrane proteins from a phage-display library

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FJ.YMETH.2012.06.012

Subtractive single-chain antibody (scFv) phage-display: tailoring phage-display for high specificity against function-specific conformations of cell membrane molecules

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FJ.YMETH.2012.06.012

Cloning antifungal single chain fragment variable antibodies by phage display and competitive panning elution

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FJ.YMETH.2012.06.012

An improved phage-display panning method to produce an HM-1 killer toxin anti-idiotypic antibody

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FJ.YMETH.2012.06.012

Selection of human antibody fragments by phage display

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FJ.YMETH.2012.06.012

Affinity-independent elution of antibody-displaying phages using cleavable DNA linker containing streptavidin beads

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FJ.YMETH.2012.06.012

Retrieval of phage displayed scFv fragments using direct bacterial elution

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FJ.YMETH.2012.06.012

Modulating the binding properties of an anti‐17β‐estradiol antibody by systematic mutation combinations

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FJ.YMETH.2012.06.012

In vitro assembly of repertoires of antibody chains on the surface of phage by renaturation

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FJ.YMETH.2012.06.012

High-throughput generation and engineering of recombinant human antibodies

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FJ.YMETH.2012.06.012

Antibodies in haystacks: how selection strategy influences the outcome of selection from molecular diversity libraries

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FJ.YMETH.2012.06.012

High-throughput generation of synthetic antibodies from highly functional minimalist phage-displayed libraries

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FJ.YMETH.2012.06.012

Towards proteome-wide production of monoclonal antibody by phage display

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FJ.YMETH.2012.06.012

Application of phage display to high throughput antibody generation and characterization

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FJ.YMETH.2012.06.012

High-throughput affinity ranking of antibodies using surface plasmon resonance microarrays

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FJ.YMETH.2012.06.012

Molecular considerations for development of phage antibody libraries.

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FJ.YMETH.2012.06.012

De novo identification of cell-type specific antibody-antigen pairs by phage display subtraction. Isolation of a human single chain antibody fragment against human keratin 14

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FJ.YMETH.2012.06.012

inferred from DOI database lookup

7 January 2021

based on heuristic

Identifiers

DOI

10.1016/J.YMETH.2012.06.012

1 reference

1 August 2017

1 reference