(Q34596533)

3 August 2017

1 reference

title

Discovery of superior enzymes by directed molecular evolution (English)

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3 August 2017

Brakmann S

1

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3 August 2017

publication date

1 December 2001

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3 August 2017

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3 August 2017

volume

2

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3 August 2017

issue

12

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3 August 2017

page(s)

865-871

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The hypercycle. Coupling of RNA and protein biosynthesis in the infection cycle of an RNA bacteriophage

3 August 2017

cites work

1 reference

https://api.crossref.org/works/10.1002%2F1439-7633%2820011203%292%3A12%3C865%3A%3AAID-CBIC865%3E3.0.CO%3B2-6

The synthesis of a self-propagating and infectious nucleic acid with a purified enzyme

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2F1439-7633%2820011203%292%3A12%3C865%3A%3AAID-CBIC865%3E3.0.CO%3B2-6

An RNA that Multiplies Indefinitely with DNA-Dependent RNA Polymerase: Selection from a Random Copolymer

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2F1439-7633%2820011203%292%3A12%3C865%3A%3AAID-CBIC865%3E3.0.CO%3B2-6

Promoters selected from random DNA sequences

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2F1439-7633%2820011203%292%3A12%3C865%3A%3AAID-CBIC865%3E3.0.CO%3B2-6

SELEXION. Systematic evolution of ligands by exponential enrichment with integrated optimization by non-linear analysis

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2F1439-7633%2820011203%292%3A12%3C865%3A%3AAID-CBIC865%3E3.0.CO%3B2-6

In vitro selection of functional nucleic acids

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2F1439-7633%2820011203%292%3A12%3C865%3A%3AAID-CBIC865%3E3.0.CO%3B2-6

In vitro selection and evolution of proteins

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2F1439-7633%2820011203%292%3A12%3C865%3A%3AAID-CBIC865%3E3.0.CO%3B2-6

Temperature adaptation of enzymes: lessons from laboratory evolution

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2F1439-7633%2820011203%292%3A12%3C865%3A%3AAID-CBIC865%3E3.0.CO%3B2-6

Statistical geometry in sequence space: a method of quantitative comparative sequence analysis

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2F1439-7633%2820011203%292%3A12%3C865%3A%3AAID-CBIC865%3E3.0.CO%3B2-6

Bacterial mutator genes and the control of spontaneous mutation

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2F1439-7633%2820011203%292%3A12%3C865%3A%3AAID-CBIC865%3E3.0.CO%3B2-6

Local mutagenesis: a method for generating viral mutants with base substitutions in preselected regions of the viral genome

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2F1439-7633%2820011203%292%3A12%3C865%3A%3AAID-CBIC865%3E3.0.CO%3B2-6

A simple and efficient method for chemical mutagenesis of DNA.

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2F1439-7633%2820011203%292%3A12%3C865%3A%3AAID-CBIC865%3E3.0.CO%3B2-6

Optimizing doped libraries by using genetic algorithms

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2F1439-7633%2820011203%292%3A12%3C865%3A%3AAID-CBIC865%3E3.0.CO%3B2-6

Scoring functions for computational algorithms applicable to the design of spiked oligonucleotides

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2F1439-7633%2820011203%292%3A12%3C865%3A%3AAID-CBIC865%3E3.0.CO%3B2-6

Exploring nonnatural evolutionary pathways by saturation mutagenesis: rapid improvement of protein function

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2F1439-7633%2820011203%292%3A12%3C865%3A%3AAID-CBIC865%3E3.0.CO%3B2-6

The advantage of sex in evolving yeast populations.

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2F1439-7633%2820011203%292%3A12%3C865%3A%3AAID-CBIC865%3E3.0.CO%3B2-6

DNA shuffling by random fragmentation and reassembly: in vitro recombination for molecular evolution

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2F1439-7633%2820011203%292%3A12%3C865%3A%3AAID-CBIC865%3E3.0.CO%3B2-6

Molecular evolution by staggered extension process (StEP) in vitro recombination

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2F1439-7633%2820011203%292%3A12%3C865%3A%3AAID-CBIC865%3E3.0.CO%3B2-6

Random-priming in vitro recombination: an effective tool for directed evolution

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2F1439-7633%2820011203%292%3A12%3C865%3A%3AAID-CBIC865%3E3.0.CO%3B2-6

DNA shuffling of a family of genes from diverse species accelerates directed evolution

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2F1439-7633%2820011203%292%3A12%3C865%3A%3AAID-CBIC865%3E3.0.CO%3B2-6

The role of peptide modules in protein evolution

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2F1439-7633%2820011203%292%3A12%3C865%3A%3AAID-CBIC865%3E3.0.CO%3B2-6

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2F1439-7633%2820011203%292%3A12%3C865%3A%3AAID-CBIC865%3E3.0.CO%3B2-6

In vitro selection and evolution of functional proteins by using ribosome display

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2F1439-7633%2820011203%292%3A12%3C865%3A%3AAID-CBIC865%3E3.0.CO%3B2-6

Man-made cell-like compartments for molecular evolution

21 January 2018

1 reference

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Selection of beta-lactamases and penicillin binding mutants from a library of phage displayed TEM-1 beta-lactamase randomly mutated in the active site omega-loop

21 January 2018

1 reference

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A method for the selection of catalytic activity using phage display and proximity coupling

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2F1439-7633%2820011203%292%3A12%3C865%3A%3AAID-CBIC865%3E3.0.CO%3B2-6

A method for directed evolution and functional cloning of enzymes

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2F1439-7633%2820011203%292%3A12%3C865%3A%3AAID-CBIC865%3E3.0.CO%3B2-6

High-throughput screening of enzyme libraries

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2F1439-7633%2820011203%292%3A12%3C865%3A%3AAID-CBIC865%3E3.0.CO%3B2-6

Confocal fluorescence coincidence analysis: an approach to ultra high-throughput screening

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2F1439-7633%2820011203%292%3A12%3C865%3A%3AAID-CBIC865%3E3.0.CO%3B2-6

Directed evolution of industrial enzymes

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2F1439-7633%2820011203%292%3A12%3C865%3A%3AAID-CBIC865%3E3.0.CO%3B2-6

Directed evolution of a para-nitrobenzyl esterase for aqueous-organic solvents

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2F1439-7633%2820011203%292%3A12%3C865%3A%3AAID-CBIC865%3E3.0.CO%3B2-6

Tuning the activity of an enzyme for unusual environments: sequential random mutagenesis of subtilisin E for catalysis in dimethylformamide

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2F1439-7633%2820011203%292%3A12%3C865%3A%3AAID-CBIC865%3E3.0.CO%3B2-6

Directed evolution of beta -glucosidase A from Paenibacillus polymyxa to thermal resistance

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2F1439-7633%2820011203%292%3A12%3C865%3A%3AAID-CBIC865%3E3.0.CO%3B2-6

Improving low-temperature catalysis in the hyperthermostable Pyrococcus furiosus beta-glucosidase CelB by directed evolution

21 January 2018

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Improved properties of FLP recombinase evolved by cycling mutagenesis

21 January 2018

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https://api.crossref.org/works/10.1002%2F1439-7633%2820011203%292%3A12%3C865%3A%3AAID-CBIC865%3E3.0.CO%3B2-6

Serial increase in the thermal stability of 3-isopropylmalate dehydrogenase from Bacillus subtilis by experimental evolution

21 January 2018

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Isolation of a thermostable enzyme variant by cloning and selection in a thermophile

21 January 2018

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Directed evolution of a thermostable esterase.

21 January 2018

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Directed evolution converts subtilisin E into a functional equivalent of thermitase

21 January 2018

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Redesigning enzyme topology by directed evolution

21 January 2018

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Functional expression of horseradish peroxidase in Saccharomyces cerevisiae and Pichia pastoris

21 January 2018

1 reference

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Progress toward the evolution of an organism with an expanded genetic code

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2F1439-7633%2820011203%292%3A12%3C865%3A%3AAID-CBIC865%3E3.0.CO%3B2-6

Directed evolution of an aspartate aminotransferase with new substrate specificities

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2F1439-7633%2820011203%292%3A12%3C865%3A%3AAID-CBIC865%3E3.0.CO%3B2-6

Redesigning the substrate specificity of an enzyme by cumulative effects of the mutations of non-active site residues

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2F1439-7633%2820011203%292%3A12%3C865%3A%3AAID-CBIC865%3E3.0.CO%3B2-6

Enhanced degradation of polychlorinated biphenyls by directed evolution of biphenyl dioxygenase

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2F1439-7633%2820011203%292%3A12%3C865%3A%3AAID-CBIC865%3E3.0.CO%3B2-6

Rapid evolution of a protein in vitro by DNA shuffling

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2F1439-7633%2820011203%292%3A12%3C865%3A%3AAID-CBIC865%3E3.0.CO%3B2-6

Laboratory evolution of peroxide-mediated cytochrome P450 hydroxylation

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2F1439-7633%2820011203%292%3A12%3C865%3A%3AAID-CBIC865%3E3.0.CO%3B2-6

Directed molecular evolution of cytochrome c peroxidase

21 January 2018

1 reference

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A genetic system to identify DNA polymerase beta mutator mutants

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2F1439-7633%2820011203%292%3A12%3C865%3A%3AAID-CBIC865%3E3.0.CO%3B2-6

Identification of residues involved in the specificity and regulation of the highly efficient multisubstrate deoxyribonucleoside kinase from Drosophila melanogaster.

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2F1439-7633%2820011203%292%3A12%3C865%3A%3AAID-CBIC865%3E3.0.CO%3B2-6

Directed evolution of a fucosidase from a galactosidase by DNA shuffling and screening

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2F1439-7633%2820011203%292%3A12%3C865%3A%3AAID-CBIC865%3E3.0.CO%3B2-6

Human immunodeficiency virus reverse transcriptase. Functional mutants obtained by random mutagenesis coupled with genetic selection in Escherichia coli

21 January 2018

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Substrate specificity of Staphylococcus hyicus lipase and Staphylococcus aureus lipase as studied by in vivo chimeragenesis

21 January 2018

1 reference

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Random mutagenesis of the substrate-binding site of a serine protease can generate enzymes with increased activities and altered primary specificities

21 January 2018

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Creation of drug-specific herpes simplex virus type 1 thymidine kinase mutants for gene therapy

21 January 2018

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Directed evolution of thymidine kinase for AZT phosphorylation using DNA family shuffling

21 January 2018

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https://api.crossref.org/works/10.1002%2F1439-7633%2820011203%292%3A12%3C865%3A%3AAID-CBIC865%3E3.0.CO%3B2-6

Inverting enantioselectivity by directed evolution of hydantoinase for improved production of L-methionine

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2F1439-7633%2820011203%292%3A12%3C865%3A%3AAID-CBIC865%3E3.0.CO%3B2-6

Directed evolution of an esterase from Pseudomonas fluorescens. Random mutagenesis by error-prone PCR or a mutator strain and identification of mutants showing enhanced enantioselectivity by a resorufin-based fluorescence assay.

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2F1439-7633%2820011203%292%3A12%3C865%3A%3AAID-CBIC865%3E3.0.CO%3B2-6

Molecular breeding of carotenoid biosynthetic pathways

21 January 2018

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Tolerance and specificity of polyketide synthases

21 January 2018

1 reference

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Directed evolution of new catalytic activity using the alpha/beta-barrel scaffold

21 January 2018

1 reference

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Directed evolution of a (beta alpha)8-barrel enzyme to catalyze related reactions in two different metabolic pathways

21 January 2018

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Molecular evolution of an arsenate detoxification pathway by DNA shuffling.

21 January 2018

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Bacterial cell surface display of an enzyme library for selective screening of improved cellulase variants

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In vitro evolution of horse heart myoglobin to increase peroxidase activity

21 January 2018

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Directed evolution of thermostable kanamycin-resistance gene: a convenient selection marker for Thermus thermophilus

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Novel human DNA alkyltransferases obtained by random substitution and genetic selection in bacteria

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Cold adaptation of a mesophilic subtilisin-like protease by laboratory evolution

21 January 2018

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DNA shuffling of subgenomic sequences of subtilisin

21 January 2018

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21 January 2018

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Towards the design of rare cutting restriction endonucleases: using directed evolution to generate variants of EcoRV differing in their substrate specificity by two orders of magnitude

21 January 2018

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Modeling DNA mutation and recombination for directed evolution experiments

21 January 2018

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Directed evolution of a fungal peroxidase

21 January 2018

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Directed evolution of the surface chemistry of the reporter enzyme beta-glucuronidase

21 January 2018

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A strategy for the isolation of catalytic activities from repertoires of enzymes displayed on phage 1 1Edited by J. Karn

21 January 2018

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Mutagenesis with degenerate oligonucleotides: an efficient method for saturating a defined DNA region with base pair substitutions

21 January 2018

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Genotypes with phenotypes: adventures in an RNA toy world

21 January 2018

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Combination of DMT-mononucleotide and Fmoc-trinucleotide phosphoramidites in oligonucleotide synthesis affords an automatable codon-level mutagenesis method

21 January 2018

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[14] Genetic applications of yeast transformation with linear and gapped plasmids

21 January 2018

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Fluorescence correlation spectroscopy (FCS)--a highly sensitive method to analyze drug/target interactions

21 January 2018

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Novel family shuffling methods for the in vitro evolution of enzymes

21 January 2018

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The effect of high-frequency random mutagenesis on in vitro protein evolution: a study on TEM-1 beta-lactamase

21 January 2018

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Directed evolution of the fatty-acid hydroxylase P450 BM-3 into an indole-hydroxylating catalyst

21 January 2018

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Directed evolution of an esterase for the stereoselective resolution of a key intermediate in the synthesis of epothilones

21 January 2018

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Nonadditivity of mutational effects on the properties of catalase I and its application to efficient directed evolution

21 January 2018

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10.1002/1439-7633(20011203)2:12<865::AID-CBIC865>3.0.CO;2-6

21 January 2018

Identifiers

DOI

1 reference

3 August 2017

1 reference