(Q38259419)

1 September 2017

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title

Experimental design for stable genetic manipulation in mammalian cell lines: lentivirus and alternatives (English)

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1 September 2017

Darren N Saunders

2

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author name string

Robert F Shearer

series ordinal

1

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1 September 2017

13 October 2014

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1 September 2017

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1 September 2017

20

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1 September 2017

1-10

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1 September 2017

1

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Doxycycline alters metabolism and proliferation of human cell lines

1 September 2017

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Impairment of the ubiquitin-proteasome system by protein aggregation

21 January 2018

1 reference

Enhancing Gene Targeting with Designed Zinc Finger Nucleases

21 January 2018

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Pooled lentiviral shRNA screening for functional genomics in mammalian cells.

21 January 2018

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Efficient design and assembly of custom TALEN and other TAL effector-based constructs for DNA targeting

21 January 2018

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Lentiviruses in gene therapy clinical research

21 January 2018

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CRISPR/Cas9 systems targeting β-globin and CCR5 genes have substantial off-target activity

21 January 2018

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Effects of doxycycline on human prostate cancer cells in vitro

21 January 2018

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Promoter traps in embryonic stem cells: a genetic screen to identify and mutate developmental genes in mice

21 January 2018

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Comparison of lentiviral vector titration methods

21 January 2018

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Tight control of gene expression in mammalian cells by tetracycline-responsive promoters

21 January 2018

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Extensive methylation of promoter sequences silences lentiviral transgene expression during stem cell differentiation in vivo

21 January 2018

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Identification and targeting of the ROSA26 locus in human embryonic stem cells.

21 January 2018

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Recognizing and avoiding siRNA off-target effects for target identification and therapeutic application

21 January 2018

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A Programmable Dual-RNA-Guided DNA Endonuclease in Adaptive Bacterial Immunity

21 January 2018

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High cleavage efficiency of a 2A peptide derived from porcine teschovirus-1 in human cell lines, zebrafish and mice

21 January 2018

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Systematic determination of the packaging limit of lentiviral vectors

21 January 2018

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Factors influencing the titer and infectivity of lentiviral vectors.

21 January 2018

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Germline transmission and tissue-specific expression of transgenes delivered by lentiviral vectors

21 January 2018

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A guide RNA sequence design platform for the CRISPR/Cas9 system for model organism genomes.

21 January 2018

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RNA-guided human genome engineering via Cas9

21 January 2018

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Evidence for clonal attenuation of growth potential in HeLa cells

21 January 2018

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Highly efficient site-specific transgenesis in cancer cell lines

21 January 2018

1 reference

A TALE nuclease architecture for efficient genome editing

21 January 2018

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A lentiviral RNAi library for human and mouse genes applied to an arrayed viral high-content screen

21 January 2018

1 reference

In vivo gene delivery and stable transduction of nondividing cells by a lentiviral vector

21 January 2018

1 reference

RNAi Codex: a portal/database for short-hairpin RNA (shRNA) gene-silencing constructs

21 January 2018

1 reference

Transduction of nondividing cells using pseudotyped defective high-titer HIV type 1 particles

21 January 2018

1 reference

Genome-scale loss-of-function screening with a lentiviral RNAi library

21 January 2018

1 reference

Selective and nonselective packaging of cellular RNAs in retrovirus particles

21 January 2018

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Cleavage of foot-and-mouth disease virus polyprotein is mediated by residues located within a 19 amino acid sequence

21 January 2018

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The Rag GTPases bind raptor and mediate amino acid signaling to mTORC1

21 January 2018

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In silico abstraction of zinc finger nuclease cleavage profiles reveals an expanded landscape of off-target sites

21 January 2018

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Titering lentiviral vectors: comparison of DNA, RNA and marker expression methods

21 January 2018

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Inhibition of VSV binding and infectivity by phosphatidylserine: Is phosphatidylserine a VSV-binding site?

21 January 2018

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A single lentiviral vector platform for microRNA-based conditional RNA interference and coordinated transgene expression

21 January 2018

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A high-throughput platform for lentiviral overexpression screening of the human ORFeome

21 January 2018

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Protein aggregation and aggregate toxicity: new insights into protein folding, misfolding diseases and biological evolution

21 January 2018

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Lentivirus-delivered stable gene silencing by RNAi in primary cells

21 January 2018

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Promoter-regulatory region of the major immediate early gene of human cytomegalovirus

21 January 2018

1 reference

Release of replication-deficient retroviruses from a packaging cell line: interaction with glioma tumor spheroids in vitro

21 January 2018

1 reference

Cre-lox-regulated conditional RNA interference from transgenes

21 January 2018

1 reference

RNA-guided genetic silencing systems in bacteria and archaea

21 January 2018

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A public genome-scale lentiviral expression library of human ORFs

21 January 2018

1 reference

Tetracycline repressor, tetR, rather than the tetR-mammalian cell transcription factor fusion derivatives, regulates inducible gene expression in mammalian cells

21 January 2018

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Self-inactivating retroviral vectors designed for transfer of whole genes into mammalian cells

21 January 2018

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Optimization of the Tet-on system to regulate interleukin 12 expression in the liver for the treatment of hepatic tumors

21 January 2018

1 reference

21 January 2018

Identifiers

DOI

10.1111/GTC.12183

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1 September 2017

1 reference

PubMed ID

25307957