(Q38810486)

6 September 2017

title

Optimized production of HIV-1 virus-like particles by transient transfection in CAP-T cells (English)

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6 September 2017

author name string

Sonia Gutiérrez-Granados

1

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6 September 2017

Laura Cervera

2

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6 September 2017

María de Las Mercedes Segura

3

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6 September 2017

Jens Wölfel

4

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6 September 2017

Francesc Gòdia

5

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6 September 2017

language of work or name

English

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publication date

19 December 2015

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Applied Microbiology and Biotechnology

6 September 2017

published in

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6 September 2017

volume

100

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6 September 2017

issue

9

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6 September 2017

page(s)

3935-3947

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Development of a scalable process for high-yield lentiviral vector production by transient transfection of HEK293 suspension cultures

6 September 2017

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1 reference

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21 January 2018

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High-density transfection with HEK-293 cells allows doubling of transient titers and removes need for a priori DNA complex formation with PEI.

21 January 2018

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Recombinant protein production by large-scale transient gene expression in mammalian cells: state of the art and future perspectives

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Generation of HIV-1 Gag VLPs by transient transfection of HEK 293 suspension cell cultures using an optimized animal-derived component free medium.

21 January 2018

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Fluorescence fluctuation spectroscopy on viral-like particles reveals variable gag stoichiometry

21 January 2018

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A high-yielding CHO transient system: coexpression of genes encoding EBNA-1 and GS enhances transient protein expression

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Transient recombinant protein expression in a human amniocyte cell line: the CAP-T® cell system

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CAP, a new human suspension cell line for influenza virus production.

21 January 2018

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100-liter transient transfection

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S-phase synchronized CHO cells show elevated transfection efficiency and expression using CaPi

21 January 2018

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Development and validation of a quantitation assay for fluorescently tagged HIV-1 virus-like particles

21 January 2018

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Serum-free suspension large-scale transient transfection of CHO cells in WAVE bioreactors.

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21 January 2018

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Large-scale transfection of mammalian cells for the fast production of recombinant protein

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21 January 2018

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21 January 2018

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21 January 2018

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Development of a generic transient transfection process at 100 L scale

21 January 2018

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Abrogation of contaminating RNA activity in HIV-1 Gag VLPs

21 January 2018

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A simple, robust and highly efficient transient expression system for producing antibodies

21 January 2018

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CAP-T cell expression system: a novel rapid and versatile human cell expression system for fast and high yield transient protein expression

21 January 2018

1 reference

https://api.crossref.org/works/10.1007%2FS00253-015-7213-X

10.1007/S00253-015-7213-X

21 January 2018

Identifiers

DOI

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Dimensions Publication ID

6 September 2017

1026728084

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1 reference