(Q40403124)

20 September 2017

Efficient delivery of nuclease proteins for genome editing in human stem cells and primary cells (English)

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20 September 2017

8

Jin-Soo Kim

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20 September 2017

9

Carlos F Barbas

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20 September 2017

Jia Liu

1

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20 September 2017

Thomas Gaj

2

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20 September 2017

Yifeng Yang

3

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20 September 2017

Nan Wang

4

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20 September 2017

Sailan Shui

5

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20 September 2017

Sojung Kim

6

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20 September 2017

Chidananda Nagamangala Kanchiswamy

7

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20 September 2017

language of work or name

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22 October 2015

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20 September 2017

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20 September 2017

10

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20 September 2017

1842-1859

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20 September 2017

11

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https://scigraph.springernature.com/pub.10.1038/nprot.2015.117

20 September 2017

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cites work

ZFN, TALEN, and CRISPR/Cas-based methods for genome engineering

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2015.117

Genome engineering with targetable nucleases

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2015.117

A guide to genome engineering with programmable nucleases

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2015.117

Expression of a site-specific endonuclease stimulates homologous recombination in mammalian cells

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2015.117

Targeted gene knockout in mammalian cells by using engineered zinc-finger nucleases.

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2015.117

Chimeric nucleases stimulate gene targeting in human cells

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2015.117

Enhancing Gene Targeting with Designed Zinc Finger Nucleases

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2015.117

Highly efficient endogenous human gene correction using designed zinc-finger nucleases

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2015.117

Hybrid restriction enzymes: zinc finger fusions to Fok I cleavage domain

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2015.117

A TALE nuclease architecture for efficient genome editing

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2015.117

DNA recognition by Cys2His2 zinc finger proteins

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2015.117

Synthetic zinc finger proteins: the advent of targeted gene regulation and genome modification technologies

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2015.117

Breaking the code of DNA binding specificity of TAL-type III effectors

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2015.117

A simple cipher governs DNA recognition by TAL effectors

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2015.117

Efficient design and assembly of custom TALEN and other TAL effector-based constructs for DNA targeting

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2015.117

A Programmable Dual-RNA-Guided DNA Endonuclease in Adaptive Bacterial Immunity

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2015.117

RNA-guided human genome engineering via Cas9

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2015.117

Multiplex genome engineering using CRISPR/Cas systems

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2015.117

RNA-programmed genome editing in human cells

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2015.117

Targeted genome engineering in human cells with the Cas9 RNA-guided endonuclease

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2015.117

Genome editing at the crossroads of delivery, specificity, and fidelity

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2015.117

Current and future delivery systems for engineered nucleases: ZFN, TALEN and RGEN.

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2015.117

Electroporation for the efficient transfection of mammalian cells with DNA.

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2015.117

Efficient transfection method for primary cells

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2015.117

Lipofection: a highly efficient, lipid-mediated DNA-transfection procedure

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2015.117

A versatile vector for gene and oligonucleotide transfer into cells in culture and in vivo: polyethylenimine

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2015.117

Cell transfection in vitro and in vivo with nontoxic TAT peptide-liposome-DNA complexes

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2015.117

Nucleofection induces non-specific changes in the metabolic activity of transfected cells.

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2015.117

Comparative transfection of DNA into primary and transformed mammalian cells from different lineages

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2015.117

In vivo genome editing restores haemostasis in a mouse model of haemophilia

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2015.117

Robust ZFN-mediated genome editing in adult hemophilic mice.

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2015.117

In vivo genome editing using Staphylococcus aureus Cas9

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2015.117

In vivo interrogation of gene function in the mammalian brain using CRISPR-Cas9

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2015.117

Directed evolution of adeno-associated virus for enhanced gene delivery and gene targeting in human pluripotent stem cells

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2015.117

Versatile and efficient genome editing in human cells by combining zinc-finger nucleases with adeno-associated viral vectors.

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2015.117

Zinc-finger nuclease-mediated gene correction using single AAV vector transduction and enhancement by Food and Drug Administration-approved drugs

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2015.117

CRISPR/Cas9-mediated genome engineering: an adeno-associated viral (AAV) vector toolbox

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2015.117

Gene editing in human stem cells using zinc finger nucleases and integrase-defective lentiviral vector delivery

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2015.117

Integrase-defective lentiviral vectors as a delivery platform for targeted modification of adenosine deaminase locus

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2015.117

Multiplex CRISPR/Cas9-based genome engineering from a single lentiviral vector

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2015.117

Generation of mouse models of myeloid malignancy with combinatorial genetic lesions using CRISPR-Cas9 genome editing.

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2015.117

Genome-scale CRISPR-Cas9 knockout screening in human cells

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2015.117

Genetic screens in human cells using the CRISPR-Cas9 system

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2015.117

Genome-Scale CRISPR-Mediated Control of Gene Repression and Activation

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2015.117

Differential integrity of TALE nuclease genes following adenoviral and lentiviral vector gene transfer into human cells.

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2015.117

Novel lentiviral vectors with mutated reverse transcriptase for mRNA delivery of TALE nucleases

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2015.117

A library of TAL effector nucleases spanning the human genome

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2015.117

Establishment of HIV-1 resistance in CD4+ T cells by genome editing using zinc-finger nucleases

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2015.117

Adenoviral vector delivery of RNA-guided CRISPR/Cas9 nuclease complexes induces targeted mutagenesis in a diverse array of human cells

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2015.117

Permanent alteration of PCSK9 with in vivo CRISPR-Cas9 genome editing

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2015.117

Efficient gene editing in adult mouse livers via adenoviral delivery of CRISPR/Cas9.

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2015.117

Adenoviral vector DNA for accurate genome editing with engineered nucleases.

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2015.117

Non-integrating gamma-retroviral vectors as a versatile tool for transient zinc-finger nuclease delivery

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2015.117

Zinc finger nuclease-expressing baculoviral vectors mediate targeted genome integration of reprogramming factor genes to facilitate the generation of human induced pluripotent stem cells

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2015.117

Baculoviral transduction facilitates TALEN-mediated targeted transgene integration and Cre/LoxP cassette exchange in human-induced pluripotent stem cells.

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2015.117

Targeted transgene insertion into the AAVS1 locus driven by baculoviral vector-mediated zinc finger nuclease expression in human-induced pluripotent stem cells.

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2015.117

Genetic rearrangements of variable di-residue (RVD)-containing repeat arrays in a baculoviral TALEN system.

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2015.117

Attenuation of zinc finger nuclease toxicity by small-molecule regulation of protein levels

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2015.117

Targeted gene knockout by direct delivery of zinc-finger nuclease proteins

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2015.117

Quantifying on- and off-target genome editing

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2015.117

Improved cell-penetrating zinc-finger nuclease proteins for precision genome engineering

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2015.117

Expression, purification and characterization of zinc-finger nuclease to knockout the goat beta-lactoglobulin gene.

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2015.117

Protein delivery using Cys2-His2 zinc-finger domains

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2015.117

Receptor-mediated delivery of engineered nucleases for genome modification

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2015.117

Targeted genome editing by lentiviral protein transduction of zinc-finger and TAL-effector nucleases

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2015.117

Cell-penetrating peptide-mediated delivery of TALEN proteins via bioconjugation for genome engineering

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2015.117

Targeted genome engineering in human induced pluripotent stem cells by penetrating TALENs

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2015.117

Highly efficient RNA-guided genome editing in human cells via delivery of purified Cas9 ribonucleoproteins

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2015.117

Cationic lipid-mediated delivery of proteins enables efficient protein-based genome editing in vitro and in vivo

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2015.117

Gene disruption by cell-penetrating peptide-mediated delivery of Cas9 protein and guide RNA.

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2015.117

Transient cold shock enhances zinc-finger nuclease-mediated gene disruption.

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2015.117

Chemically defined conditions for human iPSC derivation and culture

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2015.117

Assays for determination of protein concentration

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2015.117

Donor plasmid design for codon and single base genome editing using zinc finger nucleases.

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2015.117

Efficient targeting of expressed and silent genes in human ESCs and iPSCs using zinc-finger nucleases

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2015.117

Directed evolution of an enhanced and highly efficient FokI cleavage domain for zinc finger nucleases

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2015.117

Structure of Aart, a designed six-finger zinc finger peptide, bound to DNA.

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2015.117

The Crystal Structure of TAL Effector PthXo1 Bound to Its DNA Target

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2015.117

Crystal Structure of Cas9 in Complex with Guide RNA and Target DNA

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2015.117

Preparation of Soluble Proteins from Escherichia coli

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2015.117

A rapid and general assay for monitoring endogenous gene modification

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2015.117

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2015.117

Modular system for the construction of zinc-finger libraries and proteins

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2015.117

Targeted genome editing in human cells with zinc finger nucleases constructed via modular assembly

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2015.117

Oligomerized pool engineering (OPEN): an 'open-source' protocol for making customized zinc-finger arrays

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2015.117

Highly active zinc-finger nucleases by extended modular assembly

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2015.117

An optimized two-finger archive for ZFN-mediated gene targeting

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2015.117

Selection-free zinc-finger-nuclease engineering by context-dependent assembly (CoDA)

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2015.117

A transcription activator-like effector toolbox for genome engineering

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2015.117

FLASH assembly of TALENs for high-throughput genome editing

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2015.117

A ligation-independent cloning technique for high-throughput assembly of transcription activator–like effector genes

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2015.117

Genome engineering using the CRISPR-Cas9 system

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2015.117

Protein transduction domain of HIV-1 Tat protein promotes efficient delivery of DNA into mammalian cells

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2015.117

Direct protein delivery to mammalian cells using cell-permeable Cys2-His2 zinc-finger domains

31 March 2020

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2015.117

Enhanced homology-directed human genome engineering by controlled timing of CRISPR/Cas9 delivery

31 March 2020

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2015.117

Efficient intracellular delivery of native proteins

31 March 2020

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2015.117

High-frequency genome editing using ssDNA oligonucleotides with zinc-finger nucleases

31 March 2020

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2015.117

Preassembled zinc-finger arrays for rapid construction of ZFNs

31 March 2020

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2015.117

The Bradford method for protein quantitation

31 March 2020

1 reference

12 December 2020

Targeted chromosomal cleavage and mutagenesis in Drosophila using zinc-finger nucleases

1 reference

12 December 2020

Calcium phosphate-mediated gene transfer: a highly efficient transfection system for stably transforming cells with plasmid DNA.

1 reference

12 December 2020

The bicinchoninic acid (BCA) assay for protein quantitation

1 reference

12 December 2020

Identifiers

DOI

10.1038/NPROT.2015.117

1 reference

release_q2rnvlzve5fy3cn2rv7vijfydi

20 September 2017

1 reference

Fatcat

https://api.fatcat.wiki/v0/release/q2rnvlzve5fy3cn2rv7vijfydi

24 November 2022

mapped directly with Wikidata item

1 reference