(Q42867352)

English

Chymopapain A. Purification and investigation by covalent chromatography and characterization by two-protonic-state reactivity-probe kinetics, steady-state kinetics and resonance Raman spectroscopy of some dithioacyl derivatives

scientific article published on January 1986

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scholarly article

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Europe PubMed Central

PMC publication ID

8 November 2017

Chymopapain A. Purification and investigation by covalent chromatography and characterization by two-protonic-state reactivity-probe kinetics, steady-state kinetics and resonance Raman spectroscopy of some dithioacyl derivatives (English)

1 reference

Europe PubMed Central

PMC publication ID

8 November 2017

5

1 reference

Europe PubMed Central

PMC publication ID

8 November 2017

author name string

Baines BS

1

1 reference

Europe PubMed Central

PMC publication ID

8 November 2017

Brocklehurst K

2

1 reference

Europe PubMed Central

PMC publication ID

8 November 2017

Carey PR

3

1 reference

Europe PubMed Central

PMC publication ID

8 November 2017

Jarvis M

4

1 reference

Europe PubMed Central

PMC publication ID

8 November 2017

Storer AC

6

1 reference

Europe PubMed Central

PMC publication ID

8 November 2017

language of work or name

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publication date

1 January 1986

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Europe PubMed Central

PMC publication ID

8 November 2017

Biochemical Journal

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Europe PubMed Central

PMC publication ID

8 November 2017

233

1 reference

Europe PubMed Central

PMC publication ID

8 November 2017

1

1 reference

Europe PubMed Central

PMC publication ID

8 November 2017

119-129

1 reference

Europe PubMed Central

PMC publication ID

8 November 2017

Differences in the interaction of the catalytic groups of the active centres of actinidin and papain. Rapid purification of fully active actinidin by covalent chromatography and characterization of its active centre by use of two-protonic-state reac

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1152993

27 May 2018

Evidence for a two-state transition in papain that may have no close analogue in ficin. Differences in the disposition of cationic sites and hydrophobic binding areas in the active centres of papain and ficin

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1152993

27 May 2018

Evidence that the active centre of chymopapain A is different from the active centres of some other cysteine proteinases and that the Brønsted coefficient (beta nuc.) for the reactions of thiolate anions with 2,2'-dipyridyl disulphide may be decreas

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1152993

27 May 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1152993

27 May 2018

Chymopapain. Chromatographic purification and immunological characterization

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1152993

27 May 2018

Comparative resonance Raman spectroscopic and kinetic studies of acyl-enzymes involving papain, actinidin and papaya peptidase II

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1152993

27 May 2018

Problems of classification of papaya latex proteinases

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1152993

27 May 2018

'Chymopapain S' is chymopapain A.

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1152993

27 May 2018

Differences between the electric fields of the catalytic sites of papain and actinidin detected by using the thiol-located nitrobenzofurazan label as a spectroscopic reporter group.

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1152993

27 May 2018

A marked gradation in active-centre properties in the cysteine proteinases revealed by neutral and anionic reactivity probes. Reactivity characteristics of the thiol groups of actinidin, ficin, papain and papaya peptidase A towards 4,4'-dipyridyl di

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1152993

27 May 2018

Investigation of the catalytic site of actinidin by using benzofuroxan as a reactivity probe with selectivity for the thiolate-imidazolium ion-pair systems of cysteine proteinases. Evidence that the reaction of the ion-pair of actinidin (pKI 3.0, pK

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1152993

27 May 2018

Evidence that the lack of high catalytic activity of thiolsubtilisin towards specific substrates may be due to an inappropriately located proton-distribution system. Demonstration of highly nucleophilic character of the thiol group of thiolsubtilisi

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1152993

27 May 2018

Fractionation of proteolytic enzymes of dried papaya latex. Isolation and preliminary characterization of a new proteolytic enzyme

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1152993

27 May 2018

pH-dependence and structure-activity relationships in the papain-catalysed hydrolysis of anilides.

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1152993

27 May 2018

Kinetic specificity in papain-catalysed hydrolyses

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1152993

27 May 2018

Covalent chromatography. Preparation of fully active papain from dried papaya latex

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1152993

27 May 2018

Reactions of papain and of low-molecular-weight thiols with some aromatic disulphides. 2,2'-Dipyridyl disulphide as a convenient active-site titrant for papain even in the presence of other thiols

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1152993

27 May 2018

Mapping the active site of papain with the aid of peptide substrates and inhibitors

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1152993

27 May 2018

Fresh non-fruit latex of Carica papaya contains papain, multiple forms of chymopapain A and papaya proteinase omega

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1152993

27 May 2018

Preparation of cathepsins B and H by covalent chromatography and characterization of their catalytic sites by reaction with a thiol-specific two-protonic-state reactivity probe. Kinetic study of cathepsins B and H extending into alkaline media and a

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1152993

27 May 2018

Names and numbers of papaya proteinases

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1152993

27 May 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1152993

27 May 2018

A necessary modification to the preparation of papain from any high-quality latex of Carica papaya and evidence for the structural integrity of the enzyme produced by traditional methods

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1152993

27 May 2018

The use of resonance Raman spectroscopy to monitor catalytically important bonds during enzymic catalysis. Application to the hydrolysis of methyl thionohippurate by papain

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1152993

27 May 2018

Isolation and characterization of papaya peptidase A from commercial chymopapain

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1152993

27 May 2018

Reactivities of neutral and cationic forms of 2,2'-dipyridyl disulphide towards thiolate anions. Detection of differences between the active centres of actinidin, papain and ficin by a three-protonic-state reactivity probe

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1152993

27 May 2018

Preparation of fully active ficin from Ficus glabrata by covalent chromatography and characterization of its active centre by using 2,2'-depyridyl disulphide as a reactivity probe

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1152993

27 May 2018

DIRECT EVIDENCE FOR AN ACYLATED THIOL AS AN INTERMEDIATE IN PAPAIN- AND FICIN-CATALYSED HYDROLYSES

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1152993

16 August 2018

Characterization of papaya peptidase A as a cysteine proteinase of Carica papaya L. with active-centre properties that differ from those of papain by using 2,2'-dipyridyl disulphide and 4-chloro-7-nitrobenzofurazan as reactivity probes. Use of two-p

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1152993

16 August 2018

A re-evaluation of the nomenclature of the cysteine proteinases of Carica papaya and a rational basis for their identification

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1152993

16 August 2018

The preparation and properties of two new chromogenic substrates of trypsin

1 reference

https://pubmed.ncbi.nlm.nih.gov/3513753

12 December 2020

based on heuristic

inferred from PubMed ID database lookup

Relation between modification of functional groups of proteins and their biological activity. I.A graphical method for the determination of the number and type of essential groups

1 reference

https://pubmed.ncbi.nlm.nih.gov/3513753

12 December 2020

based on heuristic

inferred from PubMed ID database lookup

Proteolytic specificity of chymopapain: hydrolysis of the fraction B of oxidized insulin

1 reference

https://pubmed.ncbi.nlm.nih.gov/3513753

12 December 2020

based on heuristic

inferred from PubMed ID database lookup

Chymopapain. IV. The chromatographic fractionation of partially purified chymopapain and the characterization of crystalline chymopapain B

1 reference

https://pubmed.ncbi.nlm.nih.gov/3513753

12 December 2020

based on heuristic

inferred from PubMed ID database lookup

An agarose mercurial column for the separation of mercaptopapain and nonmercaptopapain

1 reference

https://pubmed.ncbi.nlm.nih.gov/3513753

12 December 2020

based on heuristic

inferred from PubMed ID database lookup

Covalent chromatography by thiol-disulfide interchange

1 reference

https://pubmed.ncbi.nlm.nih.gov/3513753

12 December 2020

based on heuristic

inferred from PubMed ID database lookup

Vibrational spectra of scissile bonds in enzyme active sites: a resonance Raman study of dithioacylpapains

1 reference

https://pubmed.ncbi.nlm.nih.gov/3513753

12 December 2020

based on heuristic

inferred from PubMed ID database lookup

24 Two-protonic-state electrophiles as probes of enzyme mechanism

1 reference

https://pubmed.ncbi.nlm.nih.gov/3513753

12 December 2020

based on heuristic

inferred from PubMed ID database lookup

Relaxed and perturbed substrate conformations in enzyme active sites: evidence from multichannel resonance raman spectra

1 reference

https://pubmed.ncbi.nlm.nih.gov/3513753

12 December 2020

based on heuristic

inferred from PubMed ID database lookup

Comparison of the substrate conformations in the active sites of papain, chymopapain, ficin and bromelain by resonance Raman spectroscopy

1 reference

https://pubmed.ncbi.nlm.nih.gov/3513753

12 December 2020

based on heuristic

inferred from PubMed ID database lookup

Identity of acyl group conformations in the active sites of papain and cathepsin B by resonance Raman spectroscopy

1 reference

https://pubmed.ncbi.nlm.nih.gov/3513753

12 December 2020

based on heuristic

inferred from PubMed ID database lookup

Identifiers

10.1042/BJ2330119

1 reference

Europe PubMed Central

PMC publication ID

8 November 2017

PMC publication ID

1 reference

Europe PubMed Central

PMC publication ID

8 November 2017

PubMed publication ID

1 reference

Europe PubMed Central

PMC publication ID

8 November 2017

ResearchGate publication ID

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