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Cryo-EM structure of Mcm2-7 double hexamer on DNA suggests a lagging-strand DNA extrusion model.
scientific article published on 25 October 2017
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Europe PubMed Central
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5692578
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https://www.ebi.ac.uk/europepmc/webservices/rest/search?query=EXT_ID:29078375%20AND%20SRC:MED&resulttype=core&format=json
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8 April 2020
title
Cryo-EM structure of Mcm2-7 double hexamer on DNA suggests a lagging-strand DNA extrusion model
(English)
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Europe PubMed Central
PMC publication ID
5692578
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https://www.ebi.ac.uk/europepmc/webservices/rest/search?query=EXT_ID:29078375%20AND%20SRC:MED&resulttype=core&format=json
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8 April 2020
main subject
cryogenic electron microscopy
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Bruce William Stillman
series ordinal
6
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Europe PubMed Central
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5692578
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https://www.ebi.ac.uk/europepmc/webservices/rest/search?query=EXT_ID:29078375%20AND%20SRC:MED&resulttype=core&format=json
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8 April 2020
Christian Speck
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7
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Europe PubMed Central
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5692578
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8 April 2020
Zuanning Yuan
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2
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5692578
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8 April 2020
Sarah Schneider
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4
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8 April 2020
author name string
Yasunori Noguchi
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1
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Europe PubMed Central
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5692578
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https://www.ebi.ac.uk/europepmc/webservices/rest/search?query=EXT_ID:29078375%20AND%20SRC:MED&resulttype=core&format=json
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8 April 2020
Lin Bai
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3
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Europe PubMed Central
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5692578
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8 April 2020
Gongpu Zhao
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5
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8 April 2020
Huilin Li
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8
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5692578
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8 April 2020
language of work or name
English
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publication date
25 October 2017
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5692578
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8 April 2020
published in
Proceedings of the National Academy of Sciences of the United States of America
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5692578
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8 April 2020
volume
114
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5692578
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8 April 2020
issue
45
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5692578
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8 April 2020
page(s)
E9529-E9538
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5692578
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8 April 2020
copyright license
Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
start time
25 October 2017
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copyright status
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A unique DNA entry gate serves for regulated loading of the eukaryotic replicative helicase MCM2-7 onto DNA
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Mec1 is one of multiple kinases that prime the Mcm2-7 helicase for phosphorylation by Cdc7
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CDK-dependent complex formation between replication proteins Dpb11, Sld2, Pol (epsilon}, and GINS in budding yeast
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The Dbf4-Cdc7 kinase promotes S phase by alleviating an inhibitory activity in Mcm4
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A double-hexameric MCM2-7 complex is loaded onto origin DNA during licensing of eukaryotic DNA replication
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Concerted loading of Mcm2-7 double hexamers around DNA during DNA replication origin licensing
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Structural biology of MCM helicases
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https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=5692578
retrieved
24 May 2018
Eukaryotic DNA replication control: lock and load, then fire
1 reference
stated in
PubMed Central
reference URL
https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=5692578
retrieved
24 May 2018
Dbf4-Cdc7 phosphorylation of Mcm2 is required for cell growth
1 reference
stated in
PubMed Central
reference URL
https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=5692578
retrieved
24 May 2018
Subunit organization of Mcm2-7 and the unequal role of active sites in ATP hydrolysis and viability.
1 reference
stated in
PubMed Central
reference URL
https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=5692578
retrieved
24 May 2018
Functional conservation of beta-hairpin DNA binding domains in the Mcm protein of Methanobacterium thermoautotrophicum and the Mcm5 protein of Saccharomyces cerevisiae.
1 reference
stated in
PubMed Central
reference URL
https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=5692578
retrieved
24 May 2018
On helicases and other motor proteins
1 reference
stated in
PubMed Central
reference URL
https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=5692578
retrieved
24 May 2018
Phosphorylation of Sld2 and Sld3 by cyclin-dependent kinases promotes DNA replication in budding yeast.
1 reference
stated in
PubMed Central
reference URL
https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=5692578
retrieved
24 May 2018
CDK-dependent phosphorylation of Sld2 and Sld3 initiates DNA replication in budding yeast
1 reference
stated in
PubMed Central
reference URL
https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=5692578
retrieved
24 May 2018
Cdc7-Dbf4 phosphorylates MCM proteins via a docking site-mediated mechanism to promote S phase progression
1 reference
stated in
PubMed Central
reference URL
https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=5692578
retrieved
24 May 2018
Mechanism of DNA translocation in a replicative hexameric helicase
1 reference
stated in
PubMed Central
reference URL
https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=5692578
retrieved
24 May 2018
Isolation of the Cdc45/Mcm2-7/GINS (CMG) complex, a candidate for the eukaryotic DNA replication fork helicase
1 reference
stated in
PubMed Central
reference URL
https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=5692578
retrieved
24 May 2018
Evolutionary relationships and structural mechanisms of AAA+ proteins
1 reference
stated in
PubMed Central
reference URL
https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=5692578
retrieved
24 May 2018
Minichromosome maintenance helicase activity is controlled by N- and C-terminal motifs and requires the ATPase domain helix-2 insert.
1 reference
stated in
PubMed Central
reference URL
https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=5692578
retrieved
24 May 2018
Pumps, paradoxes and ploughshares: mechanism of the MCM2-7 DNA helicase
1 reference
stated in
PubMed Central
reference URL
https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=5692578
retrieved
24 May 2018
Origin recognition and the chromosome cycle
1 reference
stated in
PubMed Central
reference URL
https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=5692578
retrieved
24 May 2018
ATP hydrolysis by ORC catalyzes reiterative Mcm2-7 assembly at a defined origin of replication
1 reference
stated in
PubMed Central
reference URL
https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=5692578
retrieved
24 May 2018
DNA replication in eukaryotic cells
1 reference
stated in
PubMed Central
reference URL
https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=5692578
retrieved
24 May 2018
Mcm2 is a target of regulation by Cdc7-Dbf4 during the initiation of DNA synthesis
1 reference
stated in
PubMed Central
reference URL
https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=5692578
retrieved
24 May 2018
Cell cycle regulation of the yeast Cdc7 protein kinase by association with the Dbf4 protein
1 reference
stated in
PubMed Central
reference URL
https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=5692578
retrieved
24 May 2018
Bidirectional eukaryotic DNA replication is established by quasi-symmetrical helicase loading
1 reference
stated in
PubMed Central
reference URL
https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=5692578
retrieved
15 August 2018
Open-ringed structure of the Cdt1-Mcm2-7 complex as a precursor of the MCM double hexamer
1 reference
stated in
PubMed Central
reference URL
https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=5692578
retrieved
15 August 2018
CTFFIND4: Fast and accurate defocus estimation from electron micrographs.
1 reference
stated in
PubMed Central
reference URL
https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=5692578
retrieved
15 August 2018
An ORC/Cdc6/MCM2-7 complex is formed in a multistep reaction to serve as a platform for MCM double-hexamer assembly.
1 reference
stated in
PubMed Central
reference URL
https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=5692578
retrieved
15 August 2018
Structure and mechanism of hexameric helicases
1 reference
stated in
PubMed Central
reference URL
https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=5692578
retrieved
15 August 2018
Mcm10 plays a role in functioning of the eukaryotic replicative DNA helicase, Cdc45-Mcm-GINS.
1 reference
stated in
PubMed Central
reference URL
https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=5692578
retrieved
15 August 2018
Origin association of Sld3, Sld7, and Cdc45 proteins is a key step for determination of origin-firing timing
1 reference
stated in
PubMed Central
reference URL
https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=5692578
retrieved
15 August 2018
Activation of the MCM2-7 helicase by association with Cdc45 and GINS proteins.
1 reference
stated in
PubMed Central
reference URL
https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=5692578
retrieved
15 August 2018
MCM forked substrate specificity involves dynamic interaction with the 5'-tail
1 reference
stated in
PubMed Central
reference URL
https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=5692578
retrieved
15 August 2018
Organization of the archaeal MCM complex on DNA and implications for the helicase mechanism
1 reference
stated in
PubMed
reference URL
https://pubmed.ncbi.nlm.nih.gov/29078375
retrieved
12 December 2020
based on heuristic
inferred from PubMed ID database lookup
Identifiers
DOI
10.1073/PNAS.1712537114
1 reference
stated in
Europe PubMed Central
PMC publication ID
5692578
reference URL
https://www.ebi.ac.uk/europepmc/webservices/rest/search?query=EXT_ID:29078375%20AND%20SRC:MED&resulttype=core&format=json
retrieved
8 April 2020
PMC publication ID
5692578
1 reference
stated in
Europe PubMed Central
PMC publication ID
5692578
reference URL
https://www.ebi.ac.uk/europepmc/webservices/rest/search?query=EXT_ID:29078375%20AND%20SRC:MED&resulttype=core&format=json
retrieved
8 April 2020
PubMed publication ID
29078375
1 reference
stated in
Europe PubMed Central
PMC publication ID
5692578
reference URL
https://www.ebi.ac.uk/europepmc/webservices/rest/search?query=EXT_ID:29078375%20AND%20SRC:MED&resulttype=core&format=json
retrieved
8 April 2020
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