(Q51760334)

11 April 2018

title

Role of non-specific DNA in reducing coding DNA requirement for transient gene expression with CHO and HEK-293E cells. (English)

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11 April 2018

author name string

Yashas Rajendra

1

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Divor Kiseljak

2

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Sagar Manoli

3

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Lucia Baldi

4

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David L Hacker

5

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Florian M Wurm

6

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publication date

30 March 2012

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Biotechnology and Bioengineering

published in

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volume

109

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issue

9

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page(s)

2271-2278

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Rational vector design and multi-pathway modulation of HEK 293E cells yield recombinant antibody titers exceeding 1 g/l by transient transfection under serum-free conditions

cites work

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High-density transfection with HEK-293 cells allows doubling of transient titers and removes need for a priori DNA complex formation with PEI.

21 January 2018

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Valproic acid: a viable alternative to sodium butyrate for enhancing protein expression in mammalian cell cultures

21 January 2018

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Recombinant protein production by large-scale transient gene expression in mammalian cells: state of the art and future perspectives

21 January 2018

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Purification of polyethylenimine polyplexes highlights the role of free polycations in gene transfer

21 January 2018

1 reference

A versatile vector for gene and oligonucleotide transfer into cells in culture and in vivo: polyethylenimine

21 January 2018

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Limiting factors governing protein expression following polyethylenimine-mediated gene transfer in HEK293-EBNA1 cells

21 January 2018

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Quantification of plasmid DNA copies in the nucleus after lipoplex and polyplex transfection

21 January 2018

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Generation of recombinant Chinese hamster ovary cell lines by microinjection

21 January 2018

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High-level and high-throughput recombinant protein production by transient transfection of suspension-growing human 293-EBNA1 cells

21 January 2018

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Reflections on more than 10 years of TGE approaches

21 January 2018

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Poly(ethylenimine) and its role in gene delivery

21 January 2018

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Tracking the intracellular path of poly(ethylenimine)/DNA complexes for gene delivery

21 January 2018

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Balancing protection and release of DNA: tools to address a bottleneck of non-viral gene delivery.

21 January 2018

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Transcription of plasmid DNA: influence of plasmid DNA/polyethylenimine complex formation.

21 January 2018

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Poly(ethyleneimine)-mediated large-scale transient gene expression: influence of molecular weight, polydispersity and N-propionyl groups

21 January 2018

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Role of polyplex intermediate species on gene transfer efficiency: polyethylenimine-DNA complexes and time-resolved fluorescence spectroscopy

21 January 2018

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Dilution of reporter gene with stuffer DNA does not alter the transfection efficiency of polyethylenimines

21 January 2018

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Design and gene delivery activity of modified polyethylenimines.

21 January 2018

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Intracellular routing of plasmid DNA during non-viral gene transfer.

21 January 2018

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Synthetic DNA delivery systems

21 January 2018

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Transient gene expression: recombinant protein production with suspension-adapted HEK293-EBNA cells

21 January 2018

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Orbital shaker technology for the cultivation of mammalian cells in suspension.

21 January 2018

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Large-scale transfection of mammalian cells for the fast production of recombinant protein

21 January 2018

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A simple high-yielding process for transient gene expression in CHO cells.

21 January 2018

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Analyzing real-time PCR data by the comparative C(T) method

21 January 2018

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Production of recombinant protein therapeutics in cultivated mammalian cells

21 January 2018

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21 January 2018

Identifiers

DOI

10.1002/BIT.24494

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11 April 2018