(Q28298658)

https://www.ebi.ac.uk/europepmc/webservices/rest/search?query=EXT_ID:17446890%20AND%20SRC:MED&resulttype=core&format=json

29 December 2019

title

Iterative saturation mutagenesis (ISM) for rapid directed evolution of functional enzymes (English)

1 reference

https://www.ebi.ac.uk/europepmc/webservices/rest/search?query=EXT_ID:17446890%20AND%20SRC:MED&resulttype=core&format=json

29 December 2019

0 references

José Daniel Carballeira

series ordinal

2

1 reference

https://www.ebi.ac.uk/europepmc/webservices/rest/search?query=EXT_ID:17446890%20AND%20SRC:MED&resulttype=core&format=json

29 December 2019

1

Manfred T Reetz

1 reference

https://www.ebi.ac.uk/europepmc/webservices/rest/search?query=EXT_ID:17446890%20AND%20SRC:MED&resulttype=core&format=json

29 December 2019

language of work or name

English

0 references

publication date

1 January 2007

1 reference

https://www.ebi.ac.uk/europepmc/webservices/rest/search?query=EXT_ID:17446890%20AND%20SRC:MED&resulttype=core&format=json

29 December 2019

1 reference

https://www.ebi.ac.uk/europepmc/webservices/rest/search?query=EXT_ID:17446890%20AND%20SRC:MED&resulttype=core&format=json

29 December 2019

volume

2

1 reference

https://www.ebi.ac.uk/europepmc/webservices/rest/search?query=EXT_ID:17446890%20AND%20SRC:MED&resulttype=core&format=json

29 December 2019

page(s)

891-903

1 reference

https://www.ebi.ac.uk/europepmc/webservices/rest/search?query=EXT_ID:17446890%20AND%20SRC:MED&resulttype=core&format=json

29 December 2019

issue

4

1 reference

https://www.ebi.ac.uk/europepmc/webservices/rest/search?query=EXT_ID:17446890%20AND%20SRC:MED&resulttype=core&format=json

Directed evolution converts subtilisin E into a functional equivalent of thermitase

29 December 2019

cites work

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2007.72

A statistical analysis of random mutagenesis methods used for directed protein evolution.

31 March 2020

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2007.72

Rapid evolution of a protein in vitro by DNA shuffling

31 March 2020

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2007.72

Cassette mutagenesis: an efficient method for generation of multiple mutations at defined sites

31 March 2020

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2007.72

Creation of drug-specific herpes simplex virus type 1 thymidine kinase mutants for gene therapy

31 March 2020

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2007.72

Semi-rational approaches to engineering enzyme activity: combining the benefits of directed evolution and rational design

31 March 2020

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2007.72

Focusing mutations into the P. fluorescens esterase binding site increases enantioselectivity more effectively than distant mutations

31 March 2020

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2007.72

Rapid evolution of beta-glucuronidase specificity by saturation mutagenesis of an active site loop

31 March 2020

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2007.72

Novel methods for directed evolution of enzymes: quality, not quantity

31 March 2020

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2007.72

Site-saturation mutagenesis is more efficient than DNA shuffling for the directed evolution of beta-fucosidase from beta-galactosidase

31 March 2020

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2007.72

Expanding the substrate scope of enzymes: combining mutations obtained by CASTing

31 March 2020

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2007.72

A general strategy for selecting high-affinity zinc finger proteins for diverse DNA target sites

31 March 2020

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2007.72

Active site engineering of the epoxide hydrolase from Agrobacterium radiobacter AD1 to enhance aerobic mineralization of cis-1,2-dichloroethylene in cells expressing an evolved toluene ortho-monooxygenase

31 March 2020

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2007.72

Temperature adaptation of enzymes: lessons from laboratory evolution

31 March 2020

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2007.72

Directed evolution of enzyme stability

31 March 2020

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2007.72

Structural and genetic analysis of protein stability

31 March 2020

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2007.72

The stability of proteins in extreme environments

31 March 2020

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2007.72

Relationship of protein flexibility to thermostability

31 March 2020

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2007.72

Protein flexibility and intrinsic disorder

31 March 2020

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2007.72

The crystal structure of Bacillus subtilis lipase: a minimal alpha/beta hydrolase fold enzyme

31 March 2020

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2007.72

Alternate conformations observed in catalytic serine of Bacillus subtilis lipase determined at 1.3 A resolution

31 March 2020

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2007.72

Directed evolution of Bacillus subtilis lipase A by use of enantiomeric phosphonate inhibitors: crystal structures and phage display selection

31 March 2020

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2007.72

Structure of Aspergillus niger epoxide hydrolase at 1.8 A resolution: implications for the structure and function of the mammalian microsomal class of epoxide hydrolases

31 March 2020

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2007.72

Enhancing the enantioselectivity of an epoxide hydrolase by directed evolution

31 March 2020

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2007.72

Construction of semi-randomized gene libraries with weighted oligonucleotide synthesis and PCR.

31 March 2020

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2007.72

Directed evolution of hybrid enzymes: Evolving enantioselectivity of an achiral Rh-complex anchored to a protein

31 March 2020

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2007.72

Mathematical expressions useful in the construction, description and evaluation of protein libraries.

31 March 2020

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2007.72

Strategies and computational tools for improving randomized protein libraries

31 March 2020

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2007.72

Directed Evolution of an EnantioselectiveBacillus subtilisLipase

31 March 2020

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2007.72

Protein production by auto-induction in high density shaking cultures

31 March 2020

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2007.72

Structural basis of thermostability. Analysis of stabilizing mutations in subtilisin BPN'

31 March 2020

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2007.72

Structural perturbation and compensation by directed evolution at physiological temperature leads to thermostabilization of beta-lactamase.

31 March 2020

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2007.72

Structural and functional consequences of substitutions at the Pro108-Arg14 hydrogen bond in bovine adrenodoxin

31 March 2020

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2007.72

31 March 2020

Identifiers

DOI

10.1038/NPROT.2007.72

2 references

Consolidated OpenCitations Corpus – April 2017

1016430

https://www.ebi.ac.uk/europepmc/webservices/rest/search?query=EXT_ID:17446890%20AND%20SRC:MED&resulttype=core&format=json

Dimensions Publication ID

29 December 2019

1043615282

0 references

1016430

1 reference

Consolidated OpenCitations Corpus – April 2017

Consolidated OpenCitations Corpus – April 2017

2 references

1016430

https://www.ebi.ac.uk/europepmc/webservices/rest/search?query=EXT_ID:17446890%20AND%20SRC:MED&resulttype=core&format=json