(Q30168530)

9 June 2017

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title

Glucoamylase: structure/function relationships, and protein engineering (English)

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9 June 2017

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B Svensson

8

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author name string

J Sauer

1

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9 June 2017

B W Sigurskjold

2

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9 June 2017

U Christensen

3

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9 June 2017

T P Frandsen

4

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9 June 2017

E Mirgorodskaya

5

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9 June 2017

M Harrison

6

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9 June 2017

P Roepstorff

7

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9 June 2017

language of work or name

English

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publication date

1 December 2000

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9 June 2017

19

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Biochimica et Biophysica Acta

inferred from page(s)

published in

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9 June 2017

1543

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9 June 2017

2

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9 June 2017

275-293

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Kinetic studies on gluc-amylase. II. Competition between two types of substrate having alpha-1,4 and alpha-1,6 glucosidic linkage

9 June 2017

1 reference

https://api.crossref.org/works/10.1016%2FS0167-4838%2800%2900232-6

7 January 2021

Kinetic studies on gluc-amylase. 3. The influence of pH on the rates of hydrolysis of maltose and panose

1 reference

https://api.crossref.org/works/10.1016%2FS0167-4838%2800%2900232-6

7 January 2021

Protein engineering of the relative specificity of glucoamylase from Aspergillus awamori based on sequence similarities between starch-degrading enzymes

1 reference

https://api.crossref.org/works/10.1016%2FS0167-4838%2800%2900232-6

7 January 2021

Mutational Analysis of the Roles in Catalysis and Substrate Recognition of Arginines 54 and 305, Aspartic Acid 309, and Tryptophan 317 Located at Subsites 1 and 2 in Glucoamylase from Aspergillus niger

1 reference

https://api.crossref.org/works/10.1016%2FS0167-4838%2800%2900232-6

7 January 2021

Mutational modulation of substrate bond-type specificity and thermostability of glucoamylase from Aspergillus awamori by replacement with short homologue active site sequences and thiol/disulfide engineering

1 reference

https://api.crossref.org/works/10.1016%2FS0167-4838%2800%2900232-6

7 January 2021

A classification of glycosyl hydrolases based on amino acid sequence similarities

1 reference

https://api.crossref.org/works/10.1016%2FS0167-4838%2800%2900232-6

7 January 2021

New families in the classification of glycosyl hydrolases based on amino acid sequence similarities

1 reference

https://api.crossref.org/works/10.1016%2FS0167-4838%2800%2900232-6

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FS0167-4838%2800%2900232-6

7 January 2021

Glucoamylase structural, functional, and evolutionary relationships

1 reference

https://api.crossref.org/works/10.1016%2FS0167-4838%2800%2900232-6

7 January 2021

Refined structure for the complex of 1-deoxynojirimycin with glucoamylase from Aspergillus awamori var. X100 to 2.4-A resolution

1 reference

https://api.crossref.org/works/10.1016%2FS0167-4838%2800%2900232-6

7 January 2021

Refined crystal structures of glucoamylase from Aspergillus awamori var. X100

1 reference

https://api.crossref.org/works/10.1016%2FS0167-4838%2800%2900232-6

7 January 2021

Refined structure for the complex of D-gluco-dihydroacarbose with glucoamylase from Aspergillus awamori var. X100 to 2.2 A resolution: dual conformations for extended inhibitors bound to the active site of glucoamylase

1 reference

https://api.crossref.org/works/10.1016%2FS0167-4838%2800%2900232-6

7 January 2021

Crystallographic complexes of glucoamylase with maltooligosaccharide analogs: relationship of stereochemical distortions at the nonreducing end to the catalytic mechanism

1 reference

https://api.crossref.org/works/10.1016%2FS0167-4838%2800%2900232-6

7 January 2021

Structure of glucoamylase from Saccharomycopsis fibuligera at 1.7 A resolution

1 reference

https://api.crossref.org/works/10.1016%2FS0167-4838%2800%2900232-6

7 January 2021

Solution structure of the granular starch binding domain of glucoamylase from Aspergillus niger by nuclear magnetic resonance spectroscopy

1 reference

https://api.crossref.org/works/10.1016%2FS0167-4838%2800%2900232-6

7 January 2021

Solution structure of the granular starch binding domain of Aspergillus niger glucoamylase bound to beta-cyclodextrin

1 reference

https://api.crossref.org/works/10.1016%2FS0167-4838%2800%2900232-6

7 January 2021

Thermodynamics of binding of heterobidentate ligands consisting of spacer-connected acarbose and beta-cyclodextrin to the catalytic and starch-binding domains of glucoamylase from Aspergillus niger shows that the catalytic and starch-binding sites a

1 reference

https://api.crossref.org/works/10.1016%2FS0167-4838%2800%2900232-6

7 January 2021

Dynamic Light Scattering Evidence for a Ligand-Induced Motion between the Two Domains of Glucoamylase G1 of Aspergillus niger with Heterobivalent Substrate Analogues

1 reference

https://api.crossref.org/works/10.1016%2FS0167-4838%2800%2900232-6

7 January 2021

STEREOCHEMISTRY AND THE MECHANISM OF ENZYMATIC REACTIONS

1 reference

https://api.crossref.org/works/10.1016%2FS0167-4838%2800%2900232-6

7 January 2021

Mechanisms of enzymatic glycoside hydrolysis

1 reference

https://api.crossref.org/works/10.1016%2FS0167-4838%2800%2900232-6

7 January 2021

Enzymatic properties of the cysteinesulfinic acid derivative of the catalytic-base mutant Glu400-->Cys of glucoamylase from Aspergillus awamori

1 reference

https://api.crossref.org/works/10.1016%2FS0167-4838%2800%2900232-6

7 January 2021

Subsite structure and ligand binding mechanism of glucoamylase

1 reference

https://api.crossref.org/works/10.1016%2FS0167-4838%2800%2900232-6

7 January 2021

Catalytic mechanism of fungal glucoamylase as defined by mutagenesis of Asp176, Glu179 and Glu180 in the enzyme from Aspergillus awamori

1 reference

https://api.crossref.org/works/10.1016%2FS0167-4838%2800%2900232-6

7 January 2021

Site-directed mutagenesis of the catalytic base glutamic acid 400 in glucoamylase from Aspergillus niger and of tyrosine 48 and glutamine 401, both hydrogen-bonded to the gamma-carboxylate group of glutamic acid 400.

1 reference

https://api.crossref.org/works/10.1016%2FS0167-4838%2800%2900232-6

7 January 2021

Reaction mechanisms of Trp120-->Phe and wild-type glucoamylases from Aspergillus niger. Interactions with maltooligodextrins and acarbose.

1 reference

https://api.crossref.org/works/10.1016%2FS0167-4838%2800%2900232-6

7 January 2021

Thermodynamics of inhibitor binding to mutant forms of glucoamylase from Aspergillus niger determined by isothermal titration calorimetry

1 reference

https://api.crossref.org/works/10.1016%2FS0167-4838%2800%2900232-6

7 January 2021

Substrate Binding Mechanism of Glu180→Gln, Asp176→Asn, and Wild-Type Glucoamylases fromAspergillus niger†

1 reference

https://api.crossref.org/works/10.1016%2FS0167-4838%2800%2900232-6

7 January 2021

Some details of the reaction mechanism of glucoamylase from Aspergillus niger--kinetic and structural studies on Trp52-->Phe and Trp317-->Phe mutants

1 reference

https://api.crossref.org/works/10.1016%2FS0167-4838%2800%2900232-6

7 January 2021

Improved adsorption to starch of a beta-galactosidase fusion protein containing the starch-binding domain from Aspergillus glucoamylase.

1 reference

https://api.crossref.org/works/10.1016%2FS0167-4838%2800%2900232-6

7 January 2021

Enhanced recovery and purification of Aspergillus glucoamylase from Saccharomyces cerevisiae by the addition of poly(aspartic acid) tails.

1 reference

https://api.crossref.org/works/10.1016%2FS0167-4838%2800%2900232-6

7 January 2021

Strategies for improving heterologous protein production from filamentous fungi

1 reference

https://api.crossref.org/works/10.1016%2FS0167-4838%2800%2900232-6

7 January 2021

Identification of carboxylic acid residues in glucoamylase G2 from Aspergillus niger that participate in catalysis and substrate binding

1 reference

https://api.crossref.org/works/10.1016%2FS0167-4838%2800%2900232-6

7 January 2021

Structure-function relationships in glucoamylases encoded by variant Saccharomycopsis fibuligera genes

1 reference

https://api.crossref.org/works/10.1016%2FS0167-4838%2800%2900232-6

7 January 2021

1H and 15N assignments and secondary structure of the starch-binding domain of glucoamylase from Aspergillus niger

1 reference

https://api.crossref.org/works/10.1016%2FS0167-4838%2800%2900232-6

7 January 2021

Interaction of beta-cyclodextrin with the granular starch binding domain of glucoamylase.

1 reference

https://api.crossref.org/works/10.1016%2FS0167-4838%2800%2900232-6

7 January 2021

Specificity of the binding domain of glucoamylase 1.

1 reference

https://api.crossref.org/works/10.1016%2FS0167-4838%2800%2900232-6

7 January 2021

Thermodynamics of ligand binding to the starch-binding domain of glucoamylase from Aspergillus niger.

1 reference

https://api.crossref.org/works/10.1016%2FS0167-4838%2800%2900232-6

7 January 2021

Function of conserved tryptophans in the Aspergillus niger glucoamylase 1 starch binding domain.

1 reference

https://api.crossref.org/works/10.1016%2FS0167-4838%2800%2900232-6

7 January 2021

Effect of amino acid deletions in the O-glycosylated region of Aspergillus awamori glucoamylase

1 reference

https://api.crossref.org/works/10.1016%2FS0167-4838%2800%2900232-6

7 January 2021

Functional analysis of the threonine- and serine-rich Gp-I domain of glucoamylase I from Aspergillus awamori var. kawachi

1 reference

https://api.crossref.org/works/10.1016%2FS0167-4838%2800%2900232-6

7 January 2021

Thermodynamics of reversible and irreversible unfolding and domain interactions of glucoamylase from Aspergillus niger studied by differential scanning and isothermal titration calorimetry

1 reference

https://api.crossref.org/works/10.1016%2FS0167-4838%2800%2900232-6

7 January 2021

Functional analysis of O-linked oligosaccharides in threonine/serine-rich region of Aspergillus glucoamylase by expression in mannosyltransferase-disruptants of yeast

1 reference

https://api.crossref.org/works/10.1016%2FS0167-4838%2800%2900232-6

7 January 2021

Structural studies on the O-glycosidically linked carbohydrate chains of glucoamylase G1 from Aspergillus niger.

1 reference

https://api.crossref.org/works/10.1016%2FS0167-4838%2800%2900232-6

7 January 2021

Characterization of a glucoamylase G2 from Aspergillus niger

1 reference

https://api.crossref.org/works/10.1016%2FS0167-4838%2800%2900232-6

7 January 2021

O-glycosylation in Aspergillus glucoamylase. Conformation and role in binding

1 reference

https://api.crossref.org/works/10.1016%2FS0167-4838%2800%2900232-6

7 January 2021

Overexpression and characterization of Aspergillus awamori wild-type and mutant glucoamylase secreted by the methylotrophic yeast Pichia pastoris: comparison with wild-type recombinant glucoamylase produced using Saccharomyces cerevisiae and Aspergi

1 reference

https://api.crossref.org/works/10.1016%2FS0167-4838%2800%2900232-6

7 January 2021

The raw starch binding domain of cyclodextrin glycosyltransferase from Bacillus circulans strain 251

1 reference

https://api.crossref.org/works/10.1016%2FS0167-4838%2800%2900232-6

7 January 2021

The starch-binding domain from glucoamylase disrupts the structure of starch

1 reference

https://api.crossref.org/works/10.1016%2FS0167-4838%2800%2900232-6

7 January 2021

Catalytic mechanism of enzymic glycosyl transfer

1 reference

https://api.crossref.org/works/10.1016%2FS0167-4838%2800%2900232-6

7 January 2021

The interaction of 1-fluoro-D-glucopyranosyl fluoride with glucosidases

1 reference

https://api.crossref.org/works/10.1016%2FS0167-4838%2800%2900232-6

7 January 2021

Structure and energetics of the glucoamylase-isomaltose transition-state complex probed by using modeling and deoxygenated substrates coupled with site-directed mutagenesis

1 reference

https://api.crossref.org/works/10.1016%2FS0167-4838%2800%2900232-6

7 January 2021

Structures and mechanisms of glycosyl hydrolases

1 reference

https://api.crossref.org/works/10.1016%2FS0167-4838%2800%2900232-6

7 January 2021

Effects of both shortening and lengthening the active site nucleophile of Bacillus circulans xylanase on catalytic activity.

1 reference

https://api.crossref.org/works/10.1016%2FS0167-4838%2800%2900232-6

7 January 2021

Restoration of catalytic activity beyond wild-type level in glucoamylase from Aspergillus awamori by oxidation of the Glu400-->Cys catalytic-base mutant to cysteinesulfinic acid

1 reference

https://api.crossref.org/works/10.1016%2FS0167-4838%2800%2900232-6

7 January 2021

Site-directed mutagenesis at the active site Trp120 of Aspergillus awamori glucoamylase

1 reference

https://api.crossref.org/works/10.1016%2FS0167-4838%2800%2900232-6

7 January 2021

NMR spectroscopy of exchangeable protons of glucoamylase and of complexes with inhibitors in the 9-15-ppm range

1 reference

https://api.crossref.org/works/10.1016%2FS0167-4838%2800%2900232-6

7 January 2021

Roles of the aromatic side chains in the binding of substrates, inhibitors, and cyclomalto-oligosaccharides to the glucoamylase from Aspergillus niger probed by perturbation difference spectroscopy, chemical modification, and mutagenesis

1 reference

https://api.crossref.org/works/10.1016%2FS0167-4838%2800%2900232-6

7 January 2021

Functional roles and subsite locations of Leu177, Trp178 and Asn182 of Aspergillus awamori glucoamylase determined by site-directed mutagenesis

1 reference

https://api.crossref.org/works/10.1016%2FS0167-4838%2800%2900232-6

7 January 2021

Stopped-flow kinetic studies on the binding of gluconolactone and maltose to glucoamylase

1 reference

https://api.crossref.org/works/10.1016%2FS0167-4838%2800%2900232-6

7 January 2021

Stopped-flow fluorescence and steady-state kinetic studies of ligand-binding reactions of glucoamylase from Aspergillus niger

1 reference

https://api.crossref.org/works/10.1016%2FS0167-4838%2800%2900232-6

7 January 2021

Identification of enzyme-substrate and enzyme-product complexes in the catalytic mechanism of glucoamylase from Aspergillus awamori

1 reference

https://api.crossref.org/works/10.1016%2FS0167-4838%2800%2900232-6

7 January 2021

Binding of isomaltose and maltose to the glucoamylase from Aspergillus niger, as studied by fluorescence spectrophotometry and steady-state kinetics

1 reference

https://api.crossref.org/works/10.1016%2FS0167-4838%2800%2900232-6

7 January 2021

Interpretation of dependency of rate parameters on the degree of polymerization of substrate in enzyme-catalyzed reactions. Evaluation of subsite affinities of exo-enzyme

1 reference

https://api.crossref.org/works/10.1016%2FS0167-4838%2800%2900232-6

7 January 2021

Subsite affinities of glucoamylase: examination of the validity of the subsite theory

1 reference

https://api.crossref.org/works/10.1016%2FS0167-4838%2800%2900232-6

7 January 2021

Subsite mapping of Aspergillus niger glucoamylases I and II with malto- and isomaltooligosaccharides

1 reference

https://api.crossref.org/works/10.1016%2FS0167-4838%2800%2900232-6

7 January 2021

Functional roles of the invariant aspartic acid 55, tyrosine 306, and aspartic acid 309 in glucoamylase from Aspergillus awamori studied by mutagenesis

1 reference

https://api.crossref.org/works/10.1016%2FS0167-4838%2800%2900232-6

7 January 2021

Catalytic mechanism of glucoamylase probed by mutagenesis in conjunction with hydrolysis of alpha-D-glucopyranosyl fluoride and maltooligosaccharides.

1 reference

https://api.crossref.org/works/10.1016%2FS0167-4838%2800%2900232-6

7 January 2021

Thermodynamics of inhibitor binding to the catalytic site of glucoamylase from Aspergillus niger determined by displacement titration calorimetry.

1 reference

https://api.crossref.org/works/10.1016%2FS0167-4838%2800%2900232-6

7 January 2021

Determination of the solution conformation of D-gluco-dihydroacarbose, a high-affinity inhibitor bound to glucoamylase by transferred NOE NMR spectroscopy

1 reference

https://api.crossref.org/works/10.1016%2FS0167-4838%2800%2900232-6

7 January 2021

Synthesis and glycosidase inhibitory activity of 5-thioglucopyranosylamines. Molecular modeling of complexes with glucoamylase

1 reference

https://api.crossref.org/works/10.1016%2FS0167-4838%2800%2900232-6

7 January 2021

Mass spectrometric identification of a stable catalytic cysteinesulfinic acid residue in an enzymatically active chemically modified glucoamylase mutant

1 reference

https://api.crossref.org/works/10.1016%2FS0167-4838%2800%2900232-6

7 January 2021

Complexes of glucoamylase with maltoside heteroanalogues: bound ligand conformations by use of transferred NOE experiments and molecular modeling

1 reference

https://api.crossref.org/works/10.1016%2FS0167-4838%2800%2900232-6

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FS0167-4838%2800%2900232-6

7 January 2021

Kinetic identification of a hydrogen bonding pair in the glucoamylase-maltose transition state complex

1 reference

https://api.crossref.org/works/10.1016%2FS0167-4838%2800%2900232-6

7 January 2021

Chemical mapping of the active site of the glucoamylase of Aspergillus niger

1 reference

https://api.crossref.org/works/10.1016%2FS0167-4838%2800%2900232-6

7 January 2021

Improving operating performance of glucoamylase by mutagenesis

1 reference

https://api.crossref.org/works/10.1016%2FS0167-4838%2800%2900232-6

7 January 2021

Effect of replacing helical glycine residues with alanines on reversible and irreversible stability and production of Aspergillus awamori glucoamylase

1 reference

https://api.crossref.org/works/10.1016%2FS0167-4838%2800%2900232-6

7 January 2021

Identification and elimination by site-directed mutagenesis of thermolabile aspartyl bonds in Aspergillus awamori glucoamylase

1 reference

https://api.crossref.org/works/10.1016%2FS0167-4838%2800%2900232-6

7 January 2021

Substitution of asparagine residues in Aspergillus awamori glucoamylase by site-directed mutagenesis to eliminate N-glycosylation and inactivation by deamidation

1 reference

https://api.crossref.org/works/10.1016%2FS0167-4838%2800%2900232-6

7 January 2021

Stabilization of Aspergillus awamori glucoamylase by proline substitution and combining stabilizing mutations

1 reference

https://api.crossref.org/works/10.1016%2FS0167-4838%2800%2900232-6

7 January 2021

Effect on thermostability and catalytic activity of introducing disulfide bonds into Aspergillus awamori glucoamylase

1 reference

https://api.crossref.org/works/10.1016%2FS0167-4838%2800%2900232-6

7 January 2021

Molecular cloning of a glucoamylase gene from a thermophilic Clostridium and kinetics of the cloned enzyme

1 reference

https://api.crossref.org/works/10.1016%2FS0167-4838%2800%2900232-6

7 January 2021

Complete Genome Sequence of the Methanogenic Archaeon, Methanococcus jannaschii

1 reference

https://api.crossref.org/works/10.1016%2FS0167-4838%2800%2900232-6

7 January 2021

Glucoamylase from Thermoanaerobacterium thermosaccharolyticum: Sequence studies and analysis of the macromolecular architecture of the enzyme

1 reference

https://api.crossref.org/works/10.1016%2FS0167-4838%2800%2900232-6

7 January 2021

10.1016/S0167-4838(00)00232-6

Identifiers

DOI

1 reference

9 June 2017

1 reference