(Q33301937)

25 July 2017

1 reference

title

Steering directed protein evolution: strategies to manage combinatorial complexity of mutant libraries (English)

1 reference

25 July 2017

0 references

1

Tuck Seng Wong

1 reference

25 July 2017

Danilo Roccatano

2

0 references

Ulrich Schwaneberg

series ordinal

3

object named as

Ulrich Schwaneberg

1 reference

25 July 2017

1 November 2007

1 reference

Environmental Microbiology

25 July 2017

1 reference

25 July 2017

9

1 reference

25 July 2017

2645-2659

1 reference

25 July 2017

11

1 reference

Rapid Site-Directed Mutagenesis Using Two-PCR-Generated DNA Fragments Reproducing the Plasmid Template

25 July 2017

1 reference

https://api.crossref.org/works/10.1111%2FJ.1462-2920.2007.01411.X

Altering protein specificity: techniques and applications.

21 January 2018

1 reference

https://api.crossref.org/works/10.1111%2FJ.1462-2920.2007.01411.X

ConSeq: the identification of functionally and structurally important residues in protein sequences.

21 January 2018

1 reference

https://api.crossref.org/works/10.1111%2FJ.1462-2920.2007.01411.X

A hierarchical approach to protein molecular evolution

21 January 2018

1 reference

https://api.crossref.org/works/10.1111%2FJ.1462-2920.2007.01411.X

Automated high-throughput process for site-directed mutagenesis, production, purification, and kinetic characterization of enzymes

21 January 2018

1 reference

https://api.crossref.org/works/10.1111%2FJ.1462-2920.2007.01411.X

Site-directed, Ligase-Independent Mutagenesis (SLIM): a single-tube methodology approaching 100% efficiency in 4 h.

21 January 2018

1 reference

https://api.crossref.org/works/10.1111%2FJ.1462-2920.2007.01411.X

Additivity principles in biochemistry

21 January 2018

1 reference

https://api.crossref.org/works/10.1111%2FJ.1462-2920.2007.01411.X

Selecton: a server for detecting evolutionary forces at a single amino-acid site

21 January 2018

1 reference

https://api.crossref.org/works/10.1111%2FJ.1462-2920.2007.01411.X

Multiplex-PCR-based recombination as a novel high-fidelity method for directed evolution

21 January 2018

1 reference

https://api.crossref.org/works/10.1111%2FJ.1462-2920.2007.01411.X

Site-directed protein recombination as a shortest-path problem

21 January 2018

1 reference

https://api.crossref.org/works/10.1111%2FJ.1462-2920.2007.01411.X

INSULT: a novel mutagenesis method generating high yields of closed circular mutant DNA with one primer per mutant

21 January 2018

1 reference

https://api.crossref.org/works/10.1111%2FJ.1462-2920.2007.01411.X

Statistics of protein library construction

21 January 2018

1 reference

https://api.crossref.org/works/10.1111%2FJ.1462-2920.2007.01411.X

Optimizing the search algorithm for protein engineering by directed evolution

21 January 2018

1 reference

https://api.crossref.org/works/10.1111%2FJ.1462-2920.2007.01411.X

RAISE: a simple and novel method of generating random insertion and deletion mutations

21 January 2018

1 reference

https://api.crossref.org/works/10.1111%2FJ.1462-2920.2007.01411.X

ConSurf: identification of functional regions in proteins by surface-mapping of phylogenetic information

21 January 2018

1 reference

https://api.crossref.org/works/10.1111%2FJ.1462-2920.2007.01411.X

The ConSurf-HSSP database: the mapping of evolutionary conservation among homologs onto PDB structures.

21 January 2018

1 reference

https://api.crossref.org/works/10.1111%2FJ.1462-2920.2007.01411.X

Biased mutation-assembling: an efficient method for rapid directed evolution through simultaneous mutation accumulation

21 January 2018

1 reference

https://api.crossref.org/works/10.1111%2FJ.1462-2920.2007.01411.X

Directed evolution by accumulating tailored mutations: thermostabilization of lactate oxidase with less trade-off with catalytic activity

21 January 2018

1 reference

https://api.crossref.org/works/10.1111%2FJ.1462-2920.2007.01411.X

Modified substrate specificity of pyrroloquinoline quinone glucose dehydrogenase by biased mutation assembling with optimized amino acid substitution.

21 January 2018

1 reference

https://api.crossref.org/works/10.1111%2FJ.1462-2920.2007.01411.X

Combining computational and experimental screening for rapid optimization of protein properties

21 January 2018

1 reference

https://api.crossref.org/works/10.1111%2FJ.1462-2920.2007.01411.X

General method for sequence-independent site-directed chimeragenesis

21 January 2018

1 reference

https://api.crossref.org/works/10.1111%2FJ.1462-2920.2007.01411.X

Inverting enantioselectivity of Burkholderia gladioli esterase EstB by directed and designed evolution.

21 January 2018

1 reference

https://api.crossref.org/works/10.1111%2FJ.1462-2920.2007.01411.X

Identifying functionally important mutations from phenotypically diverse sequence data

21 January 2018

1 reference

https://api.crossref.org/works/10.1111%2FJ.1462-2920.2007.01411.X

Characterization of the oligomer structure of recombinant human mannan-binding lectin

21 January 2018

1 reference

https://api.crossref.org/works/10.1111%2FJ.1462-2920.2007.01411.X

Search strategies for applied molecular evolution

21 January 2018

1 reference

https://api.crossref.org/works/10.1111%2FJ.1462-2920.2007.01411.X

Inverting enantioselectivity of Burkholderia cepacia KWI-56 lipase by combinatorial mutation and high-throughput screening using single-molecule PCR and in vitro expression

21 January 2018

1 reference

https://api.crossref.org/works/10.1111%2FJ.1462-2920.2007.01411.X

Rapid and efficient site-specific mutagenesis without phenotypic selection

21 January 2018

1 reference

https://api.crossref.org/works/10.1111%2FJ.1462-2920.2007.01411.X

ConSurf 2005: the projection of evolutionary conservation scores of residues on protein structures

21 January 2018

1 reference

https://api.crossref.org/works/10.1111%2FJ.1462-2920.2007.01411.X

Directed enzyme evolution guided by multidimensional analysis of substrate-activity space

21 January 2018

1 reference

https://api.crossref.org/works/10.1111%2FJ.1462-2920.2007.01411.X

Approaches to DNA mutagenesis: an overview

21 January 2018

1 reference

https://api.crossref.org/works/10.1111%2FJ.1462-2920.2007.01411.X

Overcoming antigenic diversity and improving vaccines using DNA shuffling and screening technologies

21 January 2018

1 reference

https://api.crossref.org/works/10.1111%2FJ.1462-2920.2007.01411.X

DNA shuffling and screening strategies for improving vaccine efficacy

21 January 2018

1 reference

https://api.crossref.org/works/10.1111%2FJ.1462-2920.2007.01411.X

Novel methods for directed evolution of enzymes: quality, not quantity

21 January 2018

1 reference

https://api.crossref.org/works/10.1111%2FJ.1462-2920.2007.01411.X

Creating multiple-crossover DNA libraries independent of sequence identity

21 January 2018

1 reference

https://api.crossref.org/works/10.1111%2FJ.1462-2920.2007.01411.X

Computational and experimental analysis of DNA shuffling

21 January 2018

1 reference

https://api.crossref.org/works/10.1111%2FJ.1462-2920.2007.01411.X

Inverting enantioselectivity by directed evolution of hydantoinase for improved production of L-methionine

21 January 2018

1 reference

https://api.crossref.org/works/10.1111%2FJ.1462-2920.2007.01411.X

Library analysis of SCHEMA-guided protein recombination

21 January 2018

1 reference

https://api.crossref.org/works/10.1111%2FJ.1462-2920.2007.01411.X

Structure-guided SCHEMA recombination of distantly related beta-lactamases

21 January 2018

1 reference

https://api.crossref.org/works/10.1111%2FJ.1462-2920.2007.01411.X

eCodonOpt: a systematic computational framework for optimizing codon usage in directed evolution experiments

21 January 2018

1 reference

https://api.crossref.org/works/10.1111%2FJ.1462-2920.2007.01411.X

Identifying residue–residue clashes in protein hybrids by using a second-order mean-field approach

21 January 2018

1 reference

https://api.crossref.org/works/10.1111%2FJ.1462-2920.2007.01411.X

Predicting crossover generation in DNA shuffling.

21 January 2018

1 reference

https://api.crossref.org/works/10.1111%2FJ.1462-2920.2007.01411.X

Random insertion and deletion of arbitrary number of bases for codon-based random mutation of DNAs

21 January 2018

1 reference

https://api.crossref.org/works/10.1111%2FJ.1462-2920.2007.01411.X

Chemical and biochemical strategies for the randomization of protein encoding DNA sequences: library construction methods for directed evolution

21 January 2018

1 reference

https://api.crossref.org/works/10.1111%2FJ.1462-2920.2007.01411.X

In silico identification of functional regions in proteins.

21 January 2018

1 reference

https://api.crossref.org/works/10.1111%2FJ.1462-2920.2007.01411.X

Natural history as a predictor of protein evolvability

21 January 2018

1 reference

https://api.crossref.org/works/10.1111%2FJ.1462-2920.2007.01411.X

Functional evolution and structural conservation in chimeric cytochromes p450: calibrating a structure-guided approach

21 January 2018

1 reference

https://api.crossref.org/works/10.1111%2FJ.1462-2920.2007.01411.X

Structure-guided recombination creates an artificial family of cytochromes P450.

21 January 2018

1 reference

https://api.crossref.org/works/10.1111%2FJ.1462-2920.2007.01411.X

Directed evolution: selecting today's biocatalysts

21 January 2018

1 reference

https://api.crossref.org/works/10.1111%2FJ.1462-2920.2007.01411.X

User-friendly algorithms for estimating completeness and diversity in randomized protein-encoding libraries

21 January 2018

1 reference

https://api.crossref.org/works/10.1111%2FJ.1462-2920.2007.01411.X

A branch-and-bound algorithm for the inference of ancestral amino-acid sequences when the replacement rate varies among sites: Application to the evolution of five gene families.

21 January 2018

1 reference

https://api.crossref.org/works/10.1111%2FJ.1462-2920.2007.01411.X

Infrared-thermographic screening of the activity and enantioselectivity of enzymes

21 January 2018

1 reference

https://api.crossref.org/works/10.1111%2FJ.1462-2920.2007.01411.X

Expanding the substrate scope of enzymes: combining mutations obtained by CASTing

21 January 2018

1 reference

https://api.crossref.org/works/10.1111%2FJ.1462-2920.2007.01411.X

Using a residue clash map to functionally characterize protein recombination hybrids.

21 January 2018

1 reference

https://api.crossref.org/works/10.1111%2FJ.1462-2920.2007.01411.X

Using multiple sequence correlation analysis to characterize functionally important protein regions

21 January 2018

1 reference

https://api.crossref.org/works/10.1111%2FJ.1462-2920.2007.01411.X

FamClash: a method for ranking the activity of engineered enzymes.

21 January 2018

1 reference

https://api.crossref.org/works/10.1111%2FJ.1462-2920.2007.01411.X

Design of combinatorial protein libraries of optimal size

21 January 2018

1 reference

https://api.crossref.org/works/10.1111%2FJ.1462-2920.2007.01411.X

IPRO: an iterative computational protein library redesign and optimization procedure

21 January 2018

1 reference

https://api.crossref.org/works/10.1111%2FJ.1462-2920.2007.01411.X

Multiple site-directed mutagenesis of more than 10 sites simultaneously and in a single round

21 January 2018

1 reference

https://api.crossref.org/works/10.1111%2FJ.1462-2920.2007.01411.X

Evolutionary information for specifying a protein fold

21 January 2018

1 reference

https://api.crossref.org/works/10.1111%2FJ.1462-2920.2007.01411.X

DNA shuffling by random fragmentation and reassembly: in vitro recombination for molecular evolution

21 January 2018

1 reference

https://api.crossref.org/works/10.1111%2FJ.1462-2920.2007.01411.X

Rapid evolution of a protein in vitro by DNA shuffling

21 January 2018

1 reference

https://api.crossref.org/works/10.1111%2FJ.1462-2920.2007.01411.X

A combinatorial approach to substrate discrimination in the P450 CYP1A subfamily

21 January 2018

1 reference

https://api.crossref.org/works/10.1111%2FJ.1462-2920.2007.01411.X

Directed evolution of oxygenases: screening systems, success stories and challenges

21 January 2018

1 reference

https://api.crossref.org/works/10.1111%2FJ.1462-2920.2007.01411.X

A new approach to 'megaprimer' polymerase chain reaction mutagenesis without an intermediate gel purification step

21 January 2018

1 reference

https://api.crossref.org/works/10.1111%2FJ.1462-2920.2007.01411.X

Directed evolution of enzymes for product chemistry.

21 January 2018

1 reference

https://api.crossref.org/works/10.1111%2FJ.1462-2920.2007.01411.X

Computational method to reduce the search space for directed protein evolution

21 January 2018

1 reference

https://api.crossref.org/works/10.1111%2FJ.1462-2920.2007.01411.X

A computer program for the estimation of protein and nucleic acid sequence diversity in random point mutagenesis libraries

21 January 2018

1 reference

https://api.crossref.org/works/10.1111%2FJ.1462-2920.2007.01411.X

Estimation of the mutation rate during error-prone polymerase chain reaction

21 January 2018

1 reference

https://api.crossref.org/works/10.1111%2FJ.1462-2920.2007.01411.X

Additivity of mutational effects in proteins

21 January 2018

1 reference

https://api.crossref.org/works/10.1111%2FJ.1462-2920.2007.01411.X

Protein sequence randomization: efficient estimation of protein stability using knowledge-based potentials

21 January 2018

1 reference

https://api.crossref.org/works/10.1111%2FJ.1462-2920.2007.01411.X

The diversity challenge in directed protein evolution

21 January 2018

1 reference

https://api.crossref.org/works/10.1111%2FJ.1462-2920.2007.01411.X

A statistical analysis of random mutagenesis methods used for directed protein evolution.

21 January 2018

1 reference

https://api.crossref.org/works/10.1111%2FJ.1462-2920.2007.01411.X

Are transversion mutations better? A Mutagenesis Assistant Program analysis on P450 BM-3 heme domain.

21 January 2018

1 reference

https://api.crossref.org/works/10.1111%2FJ.1462-2920.2007.01411.X

Sequence saturation mutagenesis (SeSaM): a novel method for directed evolution

21 January 2018

1 reference

https://api.crossref.org/works/10.1111%2FJ.1462-2920.2007.01411.X

Sequence saturation mutagenesis with tunable mutation frequencies.

21 January 2018

1 reference

https://api.crossref.org/works/10.1111%2FJ.1462-2920.2007.01411.X

A novel PCR strategy for high-efficiency, automated site-directed mutagenesis

21 January 2018

1 reference

https://api.crossref.org/works/10.1111%2FJ.1462-2920.2007.01411.X

TAMS technology for simple and efficient in vitro site-directed mutagenesis and mutant screening

21 January 2018

1 reference

https://api.crossref.org/works/10.1111%2FJ.1462-2920.2007.01411.X

Laboratory-directed protein evolution

21 January 2018

1 reference

https://api.crossref.org/works/10.1111%2FJ.1462-2920.2007.01411.X

Molecular evolution by staggered extension process (StEP) in vitro recombination

21 January 2018

1 reference

https://api.crossref.org/works/10.1111%2FJ.1462-2920.2007.01411.X

An efficient one-step site-directed and site-saturation mutagenesis protocol

21 January 2018

1 reference

https://api.crossref.org/works/10.1111%2FJ.1462-2920.2007.01411.X

A general model of error-prone PCR

21 January 2018

1 reference

https://api.crossref.org/works/10.1111%2FJ.1462-2920.2007.01411.X

Modeling DNA mutation and recombination for directed evolution experiments

21 January 2018

1 reference

https://api.crossref.org/works/10.1111%2FJ.1462-2920.2007.01411.X

Directed evolution of enantioselective enzymes: iterative cycles of CASTing for probing protein-sequence space

21 January 2018

1 reference

https://api.crossref.org/works/10.1111%2FJ.1462-2920.2007.01411.X

Iterative saturation mutagenesis on the basis of B factors as a strategy for increasing protein thermostability

21 January 2018

1 reference

https://api.crossref.org/works/10.1111%2FJ.1462-2920.2007.01411.X

An efficient site-directed mutagenesis method for ColE1-type ori plasmid

21 January 2018

1 reference

https://api.crossref.org/works/10.1111%2FJ.1462-2920.2007.01411.X

10.1111/J.1462-2920.2007.01411.X

21 January 2018

Identifiers

DOI

1 reference

25 July 2017

1 reference

PubMed ID

17922750