(Q33372370)

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Improved PCR method for the creation of saturation mutagenesis libraries in directed evolution: application to difficult-to-amplify templates

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scholarly article

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Europe PubMed Central

PubMed publication ID

26 July 2017

Improved PCR method for the creation of saturation mutagenesis libraries in directed evolution: application to difficult-to-amplify templates (English)

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Europe PubMed Central

PubMed publication ID

26 July 2017

directed evolution

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Sabrina Kille

8

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Yosephine Gumulya

object named as

Yosephine Gumulya

5

0 references

Manfred T. Reetz

17

object named as

Manfred T Reetz

1 reference

Europe PubMed Central

PubMed publication ID

26 July 2017

Joaquin Sanchis

object named as

Joaquin Sanchis

1

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ORCID Public Data File 2021

author name string

Layla Fernández

2

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Europe PubMed Central

PubMed publication ID

26 July 2017

J Daniel Carballeira

3

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Europe PubMed Central

PubMed publication ID

26 July 2017

Jullien Drone

4

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Europe PubMed Central

PubMed publication ID

26 July 2017

Horst Höbenreich

6

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Europe PubMed Central

PubMed publication ID

26 July 2017

Daniel Kahakeaw

7

1 reference

Europe PubMed Central

PubMed publication ID

26 July 2017

Renate Lohmer

9

1 reference

Europe PubMed Central

PubMed publication ID

26 July 2017

Jérôme J-P Peyralans

10

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Europe PubMed Central

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26 July 2017

John Podtetenieff

11

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Europe PubMed Central

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26 July 2017

Shreenath Prasad

12

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Europe PubMed Central

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26 July 2017

Pankaj Soni

13

1 reference

Europe PubMed Central

PubMed publication ID

26 July 2017

Andreas Taglieber

14

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Europe PubMed Central

PubMed publication ID

26 July 2017

Sheng Wu

15

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26 July 2017

Felipe E Zilly

16

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26 July 2017

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publication date

27 September 2008

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26 July 2017

Applied Microbiology and Biotechnology

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Europe PubMed Central

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26 July 2017

81

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Europe PubMed Central

PubMed publication ID

26 July 2017

2

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Europe PubMed Central

PubMed publication ID

26 July 2017

387-397

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Europe PubMed Central

PubMed publication ID

26 July 2017

Regio- and enantioselective alkane hydroxylation with engineered cytochromes P450 BM-3.

1 reference

https://pubmed.ncbi.nlm.nih.gov/18820909

12 December 2020

based on heuristic

inferred from PubMed ID database lookup

Disulfide bond in Pseudomonas aeruginosa lipase stabilizes the structure but is not required for interaction with its foldase

1 reference

https://pubmed.ncbi.nlm.nih.gov/18820909

12 December 2020

based on heuristic

inferred from PubMed ID database lookup

Complete Inversion of Enantioselectivity towards Acetylated Tertiary Alcohols by a Double Mutant of aBacillus Subtilis Esterase

1 reference

https://pubmed.ncbi.nlm.nih.gov/18820909

12 December 2020

based on heuristic

inferred from PubMed ID database lookup

Enzyme optimization: moving from blind evolution to statistical exploration of sequence-function space

1 reference

https://pubmed.ncbi.nlm.nih.gov/18820909

12 December 2020

based on heuristic

inferred from PubMed ID database lookup

Directed evolution of Pseudomonas aeruginosa lipase for improved amide-hydrolyzing activity

1 reference

https://pubmed.ncbi.nlm.nih.gov/18820909

12 December 2020

based on heuristic

inferred from PubMed ID database lookup

Advances in laboratory evolution of enzymes

1 reference

https://pubmed.ncbi.nlm.nih.gov/18820909

12 December 2020

based on heuristic

inferred from PubMed ID database lookup

Randomization of genes by PCR mutagenesis

1 reference

https://pubmed.ncbi.nlm.nih.gov/18820909

12 December 2020

based on heuristic

inferred from PubMed ID database lookup

Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic pathogen

1 reference

https://pubmed.ncbi.nlm.nih.gov/18820909

12 December 2020

based on heuristic

inferred from PubMed ID database lookup

Creating random mutagenesis libraries using megaprimer PCR of whole plasmid

1 reference

https://pubmed.ncbi.nlm.nih.gov/18820909

12 December 2020

based on heuristic

inferred from PubMed ID database lookup

Iterative saturation mutagenesis on the basis of B factors as a strategy for increasing protein thermostability

1 reference

https://pubmed.ncbi.nlm.nih.gov/18820909

12 December 2020

based on heuristic

inferred from PubMed ID database lookup

The "megaprimer" method of site-directed mutagenesis

1 reference

https://pubmed.ncbi.nlm.nih.gov/18820909

12 December 2020

based on heuristic

inferred from PubMed ID database lookup

Directed evolution of oxygenases: screening systems, success stories and challenges

1 reference

https://pubmed.ncbi.nlm.nih.gov/18820909

12 December 2020

based on heuristic

inferred from PubMed ID database lookup

Controlling the enantioselectivity of enzymes by directed evolution: practical and theoretical ramifications

1 reference

https://pubmed.ncbi.nlm.nih.gov/18820909

12 December 2020

based on heuristic

inferred from PubMed ID database lookup

Directed evolution of enantioselective enzymes: iterative cycles of CASTing for probing protein-sequence space

1 reference

https://pubmed.ncbi.nlm.nih.gov/18820909

12 December 2020

based on heuristic

inferred from PubMed ID database lookup

Phenobarbital induction of a soluble cytochrome P-450-dependent fatty acid monooxygenase in Bacillus megaterium

1 reference

https://pubmed.ncbi.nlm.nih.gov/18820909

12 December 2020

based on heuristic

inferred from PubMed ID database lookup

Recent advances in biocatalysis by directed enzyme evolution

1 reference

https://pubmed.ncbi.nlm.nih.gov/18820909

12 December 2020

based on heuristic

inferred from PubMed ID database lookup

Cytochrome P450 BM-3 evolved by random and saturation mutagenesis as an effective indole-hydroxylating catalyst

1 reference

https://pubmed.ncbi.nlm.nih.gov/18820909

12 December 2020

based on heuristic

inferred from PubMed ID database lookup

Rapid evolution of a protein in vitro by DNA shuffling

1 reference

https://pubmed.ncbi.nlm.nih.gov/18820909

12 December 2020

based on heuristic

inferred from PubMed ID database lookup

Novel methods for directed evolution of enzymes: quality, not quantity

1 reference

https://pubmed.ncbi.nlm.nih.gov/18820909

12 December 2020

based on heuristic

inferred from PubMed ID database lookup

Learning from directed evolution: Further lessons from theoretical investigations into cooperative mutations in lipase enantioselectivity

1 reference

https://pubmed.ncbi.nlm.nih.gov/18820909

12 December 2020

based on heuristic

inferred from PubMed ID database lookup

A novel megaprimed and ligase-free, PCR-based, site-directed mutagenesis method

1 reference

https://pubmed.ncbi.nlm.nih.gov/18820909

12 December 2020

based on heuristic

inferred from PubMed ID database lookup

Directed Evolution of an Enantioselective Enzyme through Combinatorial Multiple-Cassette Mutagenesis

1 reference

https://pubmed.ncbi.nlm.nih.gov/18820909

12 December 2020

based on heuristic

inferred from PubMed ID database lookup

Creating randomized amino acid libraries with the QuikChange Multi Site-Directed Mutagenesis Kit

1 reference

https://pubmed.ncbi.nlm.nih.gov/18820909

12 December 2020

based on heuristic

inferred from PubMed ID database lookup

An improved PCR-mutagenesis strategy for two-site mutagenesis or sequence swapping between related genes

1 reference

https://pubmed.ncbi.nlm.nih.gov/18820909

12 December 2020

based on heuristic

inferred from PubMed ID database lookup

An efficient one-step site-directed and site-saturation mutagenesis protocol

1 reference

https://pubmed.ncbi.nlm.nih.gov/18820909

12 December 2020

based on heuristic

inferred from PubMed ID database lookup

Expanding the substrate scope of enzymes: combining mutations obtained by CASTing

1 reference

https://pubmed.ncbi.nlm.nih.gov/18820909

12 December 2020

based on heuristic

inferred from PubMed ID database lookup

Directed evolution of an enantioselective lipase

1 reference

https://pubmed.ncbi.nlm.nih.gov/18820909

12 December 2020

based on heuristic

inferred from PubMed ID database lookup

Laboratory evolution of cytochrome p450 BM-3 monooxygenase for organic cosolvents

1 reference

https://pubmed.ncbi.nlm.nih.gov/18820909

12 December 2020

based on heuristic

inferred from PubMed ID database lookup

Altering coenzyme specificity of Pichia stipitis xylose reductase by the semi-rational approach CASTing

1 reference

https://pubmed.ncbi.nlm.nih.gov/18820909

12 December 2020

based on heuristic

inferred from PubMed ID database lookup

Directed evolution of biocatalytic processes

1 reference

https://pubmed.ncbi.nlm.nih.gov/18820909

12 December 2020

based on heuristic

inferred from PubMed ID database lookup

Iterative saturation mutagenesis (ISM) for rapid directed evolution of functional enzymes

1 reference

https://pubmed.ncbi.nlm.nih.gov/18820909

12 December 2020

based on heuristic

inferred from PubMed ID database lookup

Site-directed mutagenesis by inverse PCR.

1 reference

https://pubmed.ncbi.nlm.nih.gov/18820909

12 December 2020

based on heuristic

inferred from PubMed ID database lookup

Identifiers

10.1007/S00253-008-1678-9

1 reference

Europe PubMed Central

PubMed publication ID

26 July 2017

Dimensions Publication ID

0 references

PubMed publication ID

1 reference

Europe PubMed Central

PubMed publication ID

26 July 2017

ResearchGate publication ID

0 references

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