(Q34417984)

2 August 2017

title

Large-scale de novo DNA synthesis: technologies and applications (English)

1 reference

2 August 2017

Sriram Kosuri

1

0 references

George M. Church

object named as

George M Church

series ordinal

2

0 references

publication date

1 May 2014

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2 August 2017

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2 August 2017

volume

11

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2 August 2017

issue

5

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2 August 2017

page(s)

499-507

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The dependence of cell-free protein synthesis in E. coli upon naturally occurring or synthetic polyribonucleotides

2 August 2017

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Correcting errors in synthetic DNA through consensus shuffling

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21 January 2018

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Scalable gene synthesis by selective amplification of DNA pools from high-fidelity microchips

21 January 2018

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Parallel on-chip gene synthesis and application to optimization of protein expression.

21 January 2018

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Accurate gene synthesis with tag-directed retrieval of sequence-verified DNA molecules

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'Shotgun DNA synthesis' for the high-throughput construction of large DNA molecules

21 January 2018

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A fluorescence selection method for accurate large-gene synthesis

21 January 2018

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Multifactorial Determinants of Protein Expression in Prokaryotic Open Reading Frames

21 January 2018

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Removal of polymerase-produced mutant sequences from PCR products

21 January 2018

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Two-step total gene synthesis method

21 January 2018

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Removal of mismatched bases from synthetic genes by enzymatic mismatch cleavage

21 January 2018

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Probing the limits of genetic recoding in essential genes.

21 January 2018

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High-fidelity gene synthesis by retrieval of sequence-verified DNA identified using high-throughput pyrosequencing

21 January 2018

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Programming biological operating systems: genome design, assembly and activation

21 January 2018

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Enzymatic assembly of DNA molecules up to several hundred kilobases

21 January 2018

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SLiCE: a novel bacterial cell extract-based DNA cloning method

21 January 2018

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One-step assembly in yeast of 25 overlapping DNA fragments to form a complete synthetic Mycoplasma genitalium genome

21 January 2018

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Assembling large DNA segments in yeast.

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNMETH.2918

Circular polymerase extension cloning of complex gene libraries and pathways

21 January 2018

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Harnessing homologous recombination in vitro to generate recombinant DNA via SLIC.

21 January 2018

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A modular cloning system for standardized assembly of multigene constructs

21 January 2018

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Production of complex nucleic acid libraries using highly parallel in situ oligonucleotide synthesis

21 January 2018

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Enrichment of sequencing targets from the human genome by solution hybridization

21 January 2018

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Multiplex amplification of large sets of human exons

21 January 2018

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Solution hybrid selection with ultra-long oligonucleotides for massively parallel targeted sequencing

21 January 2018

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Specific capture and whole-genome sequencing of viruses from clinical samples

21 January 2018

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Targeted genome enrichment for efficient purification of endosymbiont DNA from host DNA.

21 January 2018

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Multiplex padlock targeted sequencing reveals human hypermutable CpG variations

21 January 2018

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Targeted bisulfite sequencing reveals changes in DNA methylation associated with nuclear reprogramming

21 January 2018

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Genome-wide identification of human RNA editing sites by parallel DNA capturing and sequencing

21 January 2018

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Digital RNA allelotyping reveals tissue-specific and allele-specific gene expression in human

21 January 2018

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Autoantigen discovery with a synthetic human peptidome

21 January 2018

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PhIP-Seq characterization of autoantibodies from patients with multiple sclerosis, type 1 diabetes and rheumatoid arthritis

21 January 2018

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Construction of a rationally designed antibody platform for sequencing-assisted selection

21 January 2018

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Large-scale interaction profiling of PDZ domains through proteomic peptide-phage display using human and viral phage peptidomes

21 January 2018

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Rapid profiling of a microbial genome using mixtures of barcoded oligonucleotides

21 January 2018

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Genome-scale CRISPR-Cas9 knockout screening in human cells

21 January 2018

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Genetic screens in human cells using the CRISPR-Cas9 system

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNMETH.2918

High-resolution analysis of DNA regulatory elements by synthetic saturation mutagenesis

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNMETH.2918

Synthetic design of strong promoters

21 January 2018

1 reference

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Causes and effects of N-terminal codon bias in bacterial genes

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNMETH.2918

Composability of regulatory sequences controlling transcription and translation in Escherichia coli

21 January 2018

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Systematic dissection and optimization of inducible enhancers in human cells using a massively parallel reporter assay

21 January 2018

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Systematic dissection of regulatory motifs in 2000 predicted human enhancers using a massively parallel reporter assay

21 January 2018

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Complex effects of nucleotide variants in a mammalian cis-regulatory element.

21 January 2018

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Massively parallel in vivo enhancer assay reveals that highly local features determine the cis-regulatory function of ChIP-seq peaks.

21 January 2018

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Massively parallel synthetic promoter assays reveal the in vivo effects of binding site variants

21 January 2018

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The DNA-encoded nucleosome organization of a eukaryotic genome

21 January 2018

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Inferring gene regulatory logic from high-throughput measurements of thousands of systematically designed promoters

21 January 2018

1 reference

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Massively parallel decoding of mammalian regulatory sequences supports a flexible organizational model

21 January 2018

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Synthesis of methyl halides from biomass using engineered microbes

21 January 2018

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Coding-sequence determinants of gene expression in Escherichia coli

21 January 2018

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Deep mutational scanning: assessing protein function on a massive scale

21 January 2018

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Deep mutational scanning of an RRM domain of the Saccharomyces cerevisiae poly(A)-binding protein

21 January 2018

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High-resolution mapping of protein sequence-function relationships

21 January 2018

1 reference

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High-throughput analysis of in vivo protein stability

21 January 2018

1 reference

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The spatial architecture of protein function and adaptation

21 January 2018

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Hot spots for allosteric regulation on protein surfaces

21 January 2018

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Refactoring bacteriophage T7

21 January 2018

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A fully decompressed synthetic bacteriophage øX174 genome assembled and archived in yeast

21 January 2018

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Refactored M13 bacteriophage as a platform for tumor cell imaging and drug delivery

21 January 2018

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Refactoring the nitrogen fixation gene cluster from Klebsiella oxytoca

21 January 2018

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Measurement and modeling of intrinsic transcription terminators

21 January 2018

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Characterization of 582 natural and synthetic terminators and quantification of their design constraints

21 January 2018

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Precise and reliable gene expression via standard transcription and translation initiation elements

21 January 2018

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Quantitative estimation of activity and quality for collections of functional genetic elements.

21 January 2018

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Genomic mining of prokaryotic repressors for orthogonal logic gates

21 January 2018

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Design of orthogonal genetic switches based on a crosstalk map of σs, anti-σs, and promoters

21 January 2018

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Chemical synthesis of poliovirus cDNA: generation of infectious virus in the absence of natural template

21 January 2018

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Characterization of the reconstructed 1918 Spanish influenza pandemic virus

21 January 2018

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Synthetic recombinant bat SARS-like coronavirus is infectious in cultured cells and in mice

21 January 2018

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Generating a synthetic genome by whole genome assembly: phiX174 bacteriophage from synthetic oligonucleotides

21 January 2018

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Generation of infectious molecular clones of simian immunodeficiency virus from fecal consensus sequences of wild chimpanzees

21 January 2018

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Genetic inactivation of poliovirus infectivity by increasing the frequencies of CpG and UpA dinucleotides within and across synonymous capsid region codons.

21 January 2018

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Identification of an infectious progenitor for the multiple-copy HERV-K human endogenous retroelements

21 January 2018

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An inactivated West Nile Virus vaccine derived from a chemically synthesized cDNA system

21 January 2018

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Live attenuated influenza virus vaccines by computer-aided rational design

21 January 2018

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Modulation of poliovirus replicative fitness in HeLa cells by deoptimization of synonymous codon usage in the capsid region.

21 January 2018

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Reconstitution of an infectious human endogenous retrovirus

21 January 2018

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Reduction of the rate of poliovirus protein synthesis through large-scale codon deoptimization causes attenuation of viral virulence by lowering specific infectivity

21 January 2018

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Synthetic poliovirus and other designer viruses: what have we learned from them?

21 January 2018

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Virus attenuation by genome-scale changes in codon pair bias

21 January 2018

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Whole-genome synthesis and characterization of viable S13-like bacteriophages

21 January 2018

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Synthetic chromosome arms function in yeast and generate phenotypic diversity by design

21 January 2018

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Folding DNA to create nanoscale shapes and patterns

21 January 2018

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Folding DNA into twisted and curved nanoscale shapes

21 January 2018

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Self-assembly of DNA into nanoscale three-dimensional shapes

21 January 2018

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Three-dimensional structures self-assembled from DNA bricks

21 January 2018

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Self-assembly of carbon nanotubes into two-dimensional geometries using DNA origami templates

21 January 2018

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A logic-gated nanorobot for targeted transport of molecular payloads

21 January 2018

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DNA as a universal substrate for chemical kinetics

21 January 2018

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Scaling up digital circuit computation with DNA strand displacement cascades

21 January 2018

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CCL2 recruits inflammatory monocytes to facilitate breast-tumour metastasis

21 January 2018

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Next-generation digital information storage in DNA.

21 January 2018

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Towards practical, high-capacity, low-maintenance information storage in synthesized DNA

21 January 2018

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One-pot assembly of a hetero-dimeric DNA origami from chip-derived staples and double-stranded scaffold.

21 January 2018

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Error correction of microchip synthesized genes using Surveyor nuclease

21 January 2018

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Total Synthesis of the Gene for an Alanine Transfer Ribonucleic Acid from Yeast

21 January 2018

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An autonomous polymerization motor powered by DNA hybridization

21 January 2018

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Neural network computation with DNA strand displacement cascades

21 January 2018

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12 December 2020

Total synthesis of a tyrosine suppressor transfer RNA gene. XVI. Enzymatic joinings to form the total 207-base pair-long DNA

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Rapid and reliable DNA assembly via ligase cycling reaction

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In situ Synthesis of DNA Microarray on Functionalized Cyclic Olefin Copolymer Substrate

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When it rains, it pores

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Principles of genetic circuit design

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Protein-mediated error correction for de novo DNA synthesis

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Nucleotides part XXXII. Synthesis of a dithymidine dinucleotide containing a 3′: 5′-internucleotidic linkage

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7 January 2021

inferred from DOI database lookup

Identifiers

DOI

10.1038/NMETH.2918

1 reference

Dimensions Publication ID

2 August 2017

1022164954

0 references

1 reference