(Q38124949)

https://www.ebi.ac.uk/europepmc/webservices/rest/search?query=EXT_ID:23895593%20AND%20SRC:MED&resulttype=core&format=json

6 March 2020

title

Using site-saturation mutagenesis to explore mechanism and substrate specificity in thiamin diphosphate-dependent enzymes (English)

1 reference

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6 March 2020

2

1 reference

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6 March 2020

author name string

Forest H Andrews

series ordinal

1

1 reference

https://www.ebi.ac.uk/europepmc/webservices/rest/search?query=EXT_ID:23895593%20AND%20SRC:MED&resulttype=core&format=json

6 March 2020

language of work or name

English

0 references

publication date

23 August 2013

1 reference

https://www.ebi.ac.uk/europepmc/webservices/rest/search?query=EXT_ID:23895593%20AND%20SRC:MED&resulttype=core&format=json

6 March 2020

1 reference

https://www.ebi.ac.uk/europepmc/webservices/rest/search?query=EXT_ID:23895593%20AND%20SRC:MED&resulttype=core&format=json

6 March 2020

volume

280

1 reference

https://www.ebi.ac.uk/europepmc/webservices/rest/search?query=EXT_ID:23895593%20AND%20SRC:MED&resulttype=core&format=json

6 March 2020

issue

24

1 reference

https://www.ebi.ac.uk/europepmc/webservices/rest/search?query=EXT_ID:23895593%20AND%20SRC:MED&resulttype=core&format=json

6 March 2020

page(s)

6395-6411

1 reference

https://www.ebi.ac.uk/europepmc/webservices/rest/search?query=EXT_ID:23895593%20AND%20SRC:MED&resulttype=core&format=json

Exploring nonnatural evolutionary pathways by saturation mutagenesis: rapid improvement of protein function

6 March 2020

cites work

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Reducing codon redundancy and screening effort of combinatorial protein libraries created by saturation mutagenesis.

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Site-saturation mutagenesis of tryptophan 116 of Saccharomyces pastorianus old yellow enzyme uncovers stereocomplementary variants

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Site saturation mutagenesis demonstrates a central role for cysteine 298 as proton donor to the catalytic site in CaHydA [FeFe]-hydrogenase

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A new perspective on thiamine catalysis

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Thiamin diphosphate catalysis: enzymic and nonenzymic covalent intermediates

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On the Mechanism of Thiamine Action. IV.1Evidence from Studies on Model Systems

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Crystal structure of the thiamin diphosphate-dependent enzyme pyruvate decarboxylase from the yeast Saccharomyces cerevisiae at 2.3 A resolution

21 January 2018

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The crystal structure of benzoylformate decarboxylase at 1.6 A resolution: diversity of catalytic residues in thiamin diphosphate-dependent enzymes

21 January 2018

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Structure and mechanism of the ThDP-dependent benzaldehyde lyase from Pseudomonas fluorescens

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The 1',4'-iminopyrimidine tautomer of thiamin diphosphate is poised for catalysis in asymmetric active centers on enzymes

21 January 2018

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Reaction mechanisms of thiamin diphosphate enzymes: defining states of ionization and tautomerization of the cofactor at individual steps.

21 January 2018

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Glyoxylate carboligase lacks the canonical active site glutamate of thiamine-dependent enzymes

21 January 2018

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Glyoxylate carboligase: a unique thiamin diphosphate-dependent enzyme that can cycle between the 4'-aminopyrimidinium and 1',4'-iminopyrimidine tautomeric forms in the absence of the conserved glutamate

21 January 2018

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Factors mediating activity, selectivity, and substrate specificity for the thiamin diphosphate-dependent enzymes benzaldehyde lyase and benzoylformate decarboxylase.

21 January 2018

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Substrate specificity in thiamin diphosphate-dependent decarboxylases.

21 January 2018

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Aspartate-27 and glutamate-473 are involved in catalysis by Zymomonas mobilis pyruvate decarboxylase

21 January 2018

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The role of His113 and His114 in pyruvate decarboxylase from Zymomonas mobilis.

21 January 2018

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Site-directed mutagenesis of the ionizable groups in the active site of Zymomonas mobilis pyruvate decarboxylase: effect on activity and pH dependence.

21 January 2018

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Catalytic acid-base groups in yeast pyruvate decarboxylase. 1. Site-directed mutagenesis and steady-state kinetic studies on the enzyme with the D28A, H114F, H115F, and E477Q substitutions

21 January 2018

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Catalytic acid-base groups in yeast pyruvate decarboxylase. 2. Insights into the specific roles of D28 and E477 from the rates and stereospecificity of formation of carboligase side products

21 January 2018

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Catalytic acid-base groups in yeast pyruvate decarboxylase. 3. A steady-state kinetic model consistent with the behavior of both wild-type and variant enzymes at all relevant pH values

21 January 2018

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Exchanging the substrate specificities of pyruvate decarboxylase from Zymomonas mobilis and benzoylformate decarboxylase from Pseudomonas putida.

21 January 2018

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Determinants of substrate specificity in KdcA, a thiamin diphosphate-dependent decarboxylase.

21 January 2018

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Characterization of a thiamin diphosphate-dependent phenylpyruvate decarboxylase from Saccharomyces cerevisiae.

21 January 2018

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Isolation and characterization of a benzoylformate decarboxylase and a NAD+/NADP+-dependent benzaldehyde dehydrogenase involved in D-phenylglycine metabolism in Pseudomonas stutzeri ST-201.

21 January 2018

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Structural and kinetic analysis of catalysis by a thiamin diphosphate-dependent enzyme, benzoylformate decarboxylase

21 January 2018

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Saturation mutagenesis of putative catalytic residues of benzoylformate decarboxylase provides a challenge to the accepted mechanism

21 January 2018

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Snapshot of a Reaction Intermediate: Analysis of Benzoylformate Decarboxylase in Complex with a Benzoylphosphonate Inhibitor †

21 January 2018

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Combinatorial alanine-scanning

21 January 2018

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A bulky hydrophobic residue is not required to maintain the V-conformation of enzyme-bound thiamin diphosphate

21 January 2018

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21 January 2018

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21 January 2018

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21 January 2018

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21 January 2018

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21 January 2018

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21 January 2018

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21 January 2018

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Binding of the coenzyme and formation of the transketolase active center

21 January 2018

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Applications of transketolases in organic synthesis.

21 January 2018

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Enhancement of the substrate scope of transketolase.

21 January 2018

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Directed evolution of transketolase activity on non-phosphorylated substrates.

21 January 2018

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Examination of substrate binding in thiamin diphosphate-dependent transketolase by protein crystallography and site-directed mutagenesis

21 January 2018

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Strain and near attack conformers in enzymic thiamin catalysis: X-ray crystallographic snapshots of bacterial transketolase in covalent complex with donor ketoses xylulose 5-phosphate and fructose 6-phosphate, and in noncovalent complex with [...]

21 January 2018

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Optimisation and evaluation of a generic microplate-based HPLC screen for transketolase activity

21 January 2018

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Refined structure of transketolase from Saccharomyces cerevisiae at 2.0 A resolution

21 January 2018

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A colorimetric assay for screening transketolase activity.

21 January 2018

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Directed evolution of transketolase substrate specificity towards an aliphatic aldehyde

21 January 2018

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α,α′-Dihydroxyketone formation using aromatic and heteroaromatic aldehydes with evolved transketolase enzymes

21 January 2018

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Development of a donor-acceptor concept for enzymatic cross-coupling reactions of aldehydes: the first asymmetric cross-benzoin condensation

21 January 2018

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Phosphonate analogues of alpha-ketoglutarate inhibit the activity of the alpha-ketoglutarate dehydrogenase complex isolated from brain and in cultured cells

21 January 2018

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Crystal structure of the E1 component of the Escherichia coli 2-oxoglutarate dehydrogenase multienzyme complex

21 January 2018

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Assignment of function to histidines 260 and 298 by engineering the E1 component of the Escherichia coli 2-oxoglutarate dehydrogenase complex; substitutions that lead to acceptance of substrates lacking the 5-carboxyl group

21 January 2018

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Steady-State Kinetics and Molecular Evolution of Escherichia coli MenD [(1R,6R)-2-Succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate Synthase], an Anomalous Thiamin Diphosphate-Dependent Decarboxylase−Carboligase

21 January 2018

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Menaquinone biosynthesis in Escherichia coli: identification of 2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate as a novel intermediate and re-evaluation of MenD activity

21 January 2018

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Specificity and Reactivity in Menaquinone Biosynthesis: The Structure of Escherichia coli MenD (2-Succinyl-5-Enolpyruvyl-6-Hydroxy-3-Cyclohexadiene-1-Carboxylate Synthase)

21 January 2018

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(S)-Selective MenD variants from Escherichia coli provide access to new functionalized chiral α-hydroxy ketones

21 January 2018

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Using substrate analogues to probe the kinetic mechanism and active site of Escherichia coli MenD.

21 January 2018

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Succinylphosphonate esters are competitive inhibitors of MenD that show active-site discrimination between homologous alpha-ketoglutarate-decarboxylating enzymes

21 January 2018

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Enzyme catalysis: removing chemically 'essential' residues by site-directed mutagenesis.

21 January 2018

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How Thiamin Diphosphate Is Activated in Enzymes

21 January 2018

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