(Q38136030)

24021764

29 August 2017

title

Polyethyleneimine-based transient gene expression processes for suspension-adapted HEK-293E and CHO-DG44 cells (English)

1 reference

29 August 2017

3

1 reference

https://www.ebi.ac.uk/europepmc/webservices/rest/search?query=EXT_ID:24021764%20AND%20SRC:MED&resulttype=core&format=json

13 June 2020

author name string

David L Hacker

1

1 reference

https://www.ebi.ac.uk/europepmc/webservices/rest/search?query=EXT_ID:24021764%20AND%20SRC:MED&resulttype=core&format=json

13 June 2020

Divor Kiseljak

2

1 reference

https://www.ebi.ac.uk/europepmc/webservices/rest/search?query=EXT_ID:24021764%20AND%20SRC:MED&resulttype=core&format=json

13 June 2020

Sarah Thurnheer

4

1 reference

https://www.ebi.ac.uk/europepmc/webservices/rest/search?query=EXT_ID:24021764%20AND%20SRC:MED&resulttype=core&format=json

13 June 2020

Lucia Baldi

5

1 reference

https://www.ebi.ac.uk/europepmc/webservices/rest/search?query=EXT_ID:24021764%20AND%20SRC:MED&resulttype=core&format=json

13 June 2020

Florian M Wurm

6

1 reference

https://www.ebi.ac.uk/europepmc/webservices/rest/search?query=EXT_ID:24021764%20AND%20SRC:MED&resulttype=core&format=json

13 June 2020

publication date

8 September 2013

1 reference

24021764

Protein Expression and Purification

29 August 2017

published in

1 reference

https://www.ebi.ac.uk/europepmc/webservices/rest/search?query=EXT_ID:24021764%20AND%20SRC:MED&resulttype=core&format=json

13 June 2020

volume

92

1 reference

https://www.ebi.ac.uk/europepmc/webservices/rest/search?query=EXT_ID:24021764%20AND%20SRC:MED&resulttype=core&format=json

13 June 2020

issue

1

1 reference

https://www.ebi.ac.uk/europepmc/webservices/rest/search?query=EXT_ID:24021764%20AND%20SRC:MED&resulttype=core&format=json

13 June 2020

page(s)

67-76

1 reference

https://www.ebi.ac.uk/europepmc/webservices/rest/search?query=EXT_ID:24021764%20AND%20SRC:MED&resulttype=core&format=json

Genetics of somatic mammalian cells. III. Long-term cultivation of euploid cells from human and animal subjects

13 June 2020

cites work

1 reference

12 December 2020

Proteoglycan-Specific Molecular Switch for RPTP Clustering and Neuronal Extension

1 reference

12 December 2020

An extracellular steric seeding mechanism for Eph-ephrin signaling platform assembly

1 reference

12 December 2020

Recombinant antibody therapeutics: the impact of glycosylation on mechanisms of action

1 reference

12 December 2020

Structural and Functional Studies of LRP6 Ectodomain Reveal a Platform for Wnt Signaling

1 reference

12 December 2020

Glycoprotein structural genomics: solving the glycosylation problem

1 reference

12 December 2020

Membrane mutants of animal cells: rapid identification of those with a primary defect in glycosylation

1 reference

12 December 2020

Insulin-like growth factor binding protein 7 exhibits tumor suppressive and vessel stabilization properties in U87MG and T98G glioblastoma cell lines

1 reference

12 December 2020

Transfecting mammalian cells: optimization of critical parameters affecting calcium-phosphate precipitate formation

1 reference

12 December 2020

A new technique for the assay of infectivity of human adenovirus 5 DNA

1 reference

12 December 2020

Baculovirus expression system for heterologous multiprotein complexes.

1 reference

12 December 2020

Enhancement of the antagonistic potency of transforming growth factor-beta receptor extracellular domains by coiled coil-induced homo- and heterodimerization

1 reference

12 December 2020

Advances in shaking technologies

1 reference

12 December 2020

Reduced glutamine concentration improves protein production in growth-arrested CHO-DG44 and HEK-293E cells

1 reference

12 December 2020

Modular mechanism of Wnt signaling inhibition by Wnt inhibitory factor 1

1 reference

12 December 2020

Epidermal growth factor and perlecan fragments produced by apoptotic endothelial cells co-ordinately activate ERK1/2-dependent antiapoptotic pathways in mesenchymal stem cells.

1 reference

12 December 2020

Specific recognition of the viral protein UL18 by CD85j/LIR-1/ILT2 on CD8+ T cells mediates the non-MHC-restricted lysis of human cytomegalovirus-infected cells

1 reference

12 December 2020

On the optimal ratio of heavy to light chain genes for efficient recombinant antibody production by CHO cells.

1 reference

12 December 2020

Large-scale transfection of mammalian cells

1 reference

12 December 2020

Trends in glycosylation, glycoanalysis and glycoengineering of therapeutic antibodies and Fc-fusion proteins

1 reference

12 December 2020

A time- and cost-efficient system for high-level protein production in mammalian cells

1 reference

12 December 2020

Chemical and Structural Analysis of an Antibody Folding Intermediate Trapped during Glycan Biosynthesis

1 reference

12 December 2020

Trimeric HIV-1 glycoprotein gp140 immunogens and native HIV-1 envelope glycoproteins display the same closed and open quaternary molecular architectures

1 reference

12 December 2020

Small-scale bioreactor system for process development and optimization

1 reference

12 December 2020

Serum-free suspension large-scale transient transfection of CHO cells in WAVE bioreactors.

1 reference

12 December 2020

Mild hypothermia improves transient gene expression yields several fold in Chinese hamster ovary cells

1 reference

12 December 2020

Overexpressing human membrane proteins in stably transfected and clonal human embryonic kidney 293S cells

1 reference

12 December 2020

The human CST complex is a terminator of telomerase activity

1 reference

12 December 2020

Highly Conserved Protective Epitopes on Influenza B Viruses

1 reference

12 December 2020

Crystal structure of the GluR2 amino-terminal domain provides insights into the architecture and assembly of ionotropic glutamate receptors

1 reference

12 December 2020

Possible common central pathway for resistin and insulin in regulating food intake

1 reference

12 December 2020

Baculovirus as versatile vectors for protein expression in insect and mammalian cells

1 reference

12 December 2020

Disposable bioreactors: the current state-of-the-art and recommended applications in biotechnology

1 reference

12 December 2020

Transient gene expression levels from multigene expression vectors.

1 reference

12 December 2020

Limiting factors governing protein expression following polyethylenimine-mediated gene transfer in HEK293-EBNA1 cells

1 reference

12 December 2020

Reflections on more than 10 years of TGE approaches

1 reference

12 December 2020

Episomal vectors for gene expression in mammalian cells

1 reference

12 December 2020

LGI1 is a Nogo receptor 1 ligand that antagonizes myelin-based growth inhibition

1 reference

12 December 2020

Use of orbital shaken disposable bioreactors for mammalian cell cultures from the milliliter-scale to the 1,000-liter scale.

1 reference

12 December 2020

Serum-free transient protein production system based on adenoviral vector and PER.C6 technology: high yield and preserved bioactivity

1 reference

12 December 2020

High-level and high-throughput recombinant protein production by transient transfection of suspension-growing human 293-EBNA1 cells

1 reference

12 December 2020

Valproic acid: a viable alternative to sodium butyrate for enhancing protein expression in mammalian cell cultures

1 reference

12 December 2020

Serum-free large-scale transient transfection of CHO cells

1 reference

12 December 2020

Large-scale transient expression of therapeutic proteins in mammalian cells

1 reference

12 December 2020

Orbital shaker technology for the cultivation of mammalian cells in suspension.

1 reference

12 December 2020

Genetics of somatic mammalian cells, VII. Induction and isolation of nutritional mutants in Chinese hamster cells

1 reference

12 December 2020

Efficient expression screening of human membrane proteins in transiently transfected Human Embryonic Kidney 293S cells.

1 reference

12 December 2020

Scalable transient gene expression in Chinese hamster ovary cells in instrumented and non-instrumented cultivation systems

1 reference

12 December 2020

Large-scale transfection of mammalian cells for the fast production of recombinant protein

1 reference

12 December 2020

Production of protein complexes via co-expression

1 reference

12 December 2020

Human antibody effector function

1 reference

12 December 2020

Efficient and reproducible mammalian cell bioprocesses without probes and controllers?

1 reference

12 December 2020

Optimisation of protein expression and establishment of the Wave Bioreactor for Baculovirus/insect cell culture

1 reference

12 December 2020

Recent advances in the production of proteins in insect and mammalian cells for structural biology

1 reference

12 December 2020

Chinese hamster ovary cells

1 reference

12 December 2020

High-density transfection with HEK-293 cells allows doubling of transient titers and removes need for a priori DNA complex formation with PEI.

1 reference

12 December 2020

Production of recombinant protein therapeutics in cultivated mammalian cells

1 reference

12 December 2020

Automation of large scale transient protein expression in mammalian cells

1 reference

12 December 2020

Optimal and consistent protein glycosylation in mammalian cell culture

1 reference

12 December 2020

A highly conserved neutralizing epitope on group 2 influenza A viruses

1 reference

12 December 2020

A simple high-yielding process for transient gene expression in CHO cells.

1 reference

12 December 2020

The synthesis and high-level expression of a beta2-adrenergic receptor gene in a tetracycline-inducible stable mammalian cell line

1 reference

12 December 2020

Structure of active dimeric human telomerase

1 reference

12 December 2020

A mammalian host-vector system that regulates expression and amplification of transfected genes by temperature induction

1 reference

12 December 2020

Particle stress in bioreactors

1 reference

12 December 2020

Control of carbohydrate processing: the lec1A CHO mutation results in partial loss of N-acetylglucosaminyltransferase I activity

1 reference

12 December 2020

Transient expression technologies: past, present, and future

1 reference

12 December 2020

Control of culture environment for improved polyethylenimine-mediated transient production of recombinant monoclonal antibodies by CHO cells.

1 reference

12 December 2020

Development of a shaking bioreactor system for animal cell cultures.

1 reference

12 December 2020

Engineering antigens for in situ erythrocyte binding induces T-cell deletion

1 reference

12 December 2020

Deletion of the diploid dihydrofolate reductase locus from cultured mammalian cells

1 reference

12 December 2020

Multi-host expression system for recombinant production of challenging proteins.

1 reference

12 December 2020

Poly(ethyleneimine)-mediated large-scale transient gene expression: influence of molecular weight, polydispersity and N-propionyl groups

1 reference

12 December 2020

Transient transfection of serum-free suspension HEK 293 cell culture for efficient production of human rFVIII.

1 reference

12 December 2020

Expressing full-length functional PfEMP1 proteins in the HEK293 expression system.

1 reference

12 December 2020

Carbohydrate and domain architecture of an immature antibody glycoform exhibiting enhanced effector functions

1 reference

12 December 2020

k(L)a as a predictor for successful probe-independent mammalian cell bioprocesses in orbitally shaken bioreactors.

1 reference

12 December 2020

Recombinant protein production by large-scale transient gene expression in mammalian cells: state of the art and future perspectives

1 reference

12 December 2020

Structural basis of Nipah and Hendra virus attachment to their cell-surface receptor ephrin-B2

1 reference

12 December 2020

Molecular analysis of receptor protein tyrosine phosphatase mu-mediated cell adhesion

1 reference

12 December 2020

Structure of the Repulsive Guidance Molecule (RGM)-Neogenin Signaling Hub

1 reference

12 December 2020

Transient gene expression in suspension HEK-293 cells: application to large-scale protein production

1 reference

12 December 2020

A versatile vector for gene and oligonucleotide transfer into cells in culture and in vivo: polyethylenimine

1 reference

12 December 2020

Transient production of recombinant proteins by Chinese hamster ovary cells using polyethyleneimine/DNA complexes in combination with microtubule disrupting anti-mitotic agents

1 reference

12 December 2020

MultiBac: expanding the research toolbox for multiprotein complexes.

1 reference

12 December 2020

Scalable production of adeno-associated virus type 2 vectors via suspension transfection

1 reference

12 December 2020

The cell motility modulator Slit2 is a potent inhibitor of platelet function

1 reference

12 December 2020

Multiplexed expression and screening for recombinant protein production in mammalian cells

1 reference

12 December 2020

Development of a scalable process for high-yield lentiviral vector production by transient transfection of HEK293 suspension cultures

1 reference

12 December 2020

Stable antibody expression at therapeutic levels using the 2A peptide

1 reference

12 December 2020

Function of human Rh based on structure of RhCG at 2.1 A

1 reference

12 December 2020

The crystal structure of ORF-9b, a lipid binding protein from the SARS coronavirus.

1 reference

12 December 2020

Partial enzymatic deglycosylation preserves the structure of cleaved recombinant HIV-1 envelope glycoprotein trimers

1 reference

12 December 2020

Structural basis for cell surface patterning through NetrinG-NGL interactions

1 reference

12 December 2020

Rational vector design and multi-pathway modulation of HEK 293E cells yield recombinant antibody titers exceeding 1 g/l by transient transfection under serum-free conditions

1 reference

12 December 2020

A simplified polyethylenimine-mediated transfection process for large-scale and high-throughput applications

1 reference

12 December 2020

Large-scale transient transfection of serum-free suspension-growing HEK293 EBNA1 cells: peptone additives improve cell growth and transfection efficiency

1 reference

12 December 2020

Glycoproteins from insect cells: sialylated or not?

1 reference

12 December 2020

Epstein-Barr virus-derived vectors for transient and stable expression of recombinant proteins.

1 reference

12 December 2020

Structural insights into hedgehog ligand sequestration by the human hedgehog-interacting protein HHIP

1 reference

12 December 2020

Balancing GFP reporter plasmid quantity in large-scale transient transfections for recombinant anti-human Rhesus-D IgG1 synthesis

1 reference

12 December 2020

Chemical Macrocyclization of Peptides Fused to Antibody Fc Fragments

1 reference

12 December 2020

Transcriptome profiling of a TGF-beta-induced epithelial-to-mesenchymal transition reveals extracellular clusterin as a target for therapeutic antibodies.

1 reference

12 December 2020

Effects of pCIneo and pCEP4 expression vectors on transient and stable protein production in human and simian cell lines

1 reference

12 December 2020

Epi-CHO, an episomal expression system for recombinant protein production in CHO cells

1 reference

12 December 2020

Role of non-specific DNA in reducing coding DNA requirement for transient gene expression with CHO and HEK-293E cells.

1 reference

12 December 2020

Expression and purification of full-length mouse CARM1 from transiently transfected HEK293T cells using HaloTag technology

1 reference

12 December 2020

The axonal repellent, Slit2, inhibits directional migration of circulating neutrophils

1 reference

12 December 2020

Transient gene expression: recombinant protein production with suspension-adapted HEK293-EBNA cells

1 reference

12 December 2020

Structural basis of semaphorin-plexin signalling

1 reference

12 December 2020

Combined inhibition of PDGF and VEGF receptors by ellagic acid, a dietary-derived phenolic compound

1 reference

12 December 2020

High-titer, serum-free production of adeno-associated virus vectors by polyethyleneimine-mediated plasmid transfection in mammalian suspension cells

1 reference

12 December 2020

Glycan variability on a recombinant IgG antibody transiently produced in HEK-293E cells.

1 reference

12 December 2020

Versatile expression system for rapid and stable production of recombinant proteins

1 reference

12 December 2020

Transient recombinant protein expression in a human amniocyte cell line: the CAP-T® cell system

1 reference

12 December 2020

Purification and characterization of a recombinant G-protein-coupled receptor, Saccharomyces cerevisiae Ste2p, transiently expressed in HEK293 EBNA1 cells

1 reference

12 December 2020

Innovative, non-stirred bioreactors in scales from milliliters up to 1000 liters for suspension cultures of cells using disposable bags and containers--a Swiss contribution

1 reference

12 December 2020

Disposable bioreactor for cell culture using wave-induced agitation

1 reference

12 December 2020

Overexpression of membrane proteins in mammalian cells for structural studies

1 reference

12 December 2020

High-density transient gene expression in suspension-adapted 293 EBNA1 cells

1 reference

12 December 2020

Enhanced CHO cell-based transient gene expression with the epi-CHO expression system

1 reference

12 December 2020

High-level protein expression in scalable CHO transient transfection

1 reference

12 December 2020

Respiratory syncytial virus subunit vaccine based on a recombinant fusion protein expressed transiently in mammalian cells

1 reference

12 December 2020

The bioactivity and receptor affinity of recombinant tagged EGF designed for tissue engineering applications is defined by the nature and position of the tags

1 reference

12 December 2020

Structure and function in rhodopsin: high-level expression of rhodopsin with restricted and homogeneous N-glycosylation by a tetracycline-inducible N-acetylglucosaminyltransferase I-negative HEK293S stable mammalian cell line

1 reference

12 December 2020

Sustained activation and tumor targeting of NKT cells using a CD1d-anti-HER2-scFv fusion protein induce antitumor effects in mice

1 reference

12 December 2020

Transient expression and purification of chimeric heavy chain antibodies

1 reference

12 December 2020

Structural Plasticity of Eph Receptor A4 Facilitates Cross-Class Ephrin Signaling

1 reference

12 December 2020

Recombinant protein production by transient gene transfer into Mammalian cells

1 reference

12 December 2020

Structure of a tyrosine phosphatase adhesive interaction reveals a spacer-clamp mechanism

1 reference

12 December 2020

Neuropilins lock secreted semaphorins onto plexins in a ternary signaling complex

1 reference

12 December 2020

Production of chimeric heavy-chain antibodies

1 reference

12 December 2020

Stable replication of plasmids derived from Epstein-Barr virus in various mammalian cells

1 reference

12 December 2020

Cell line specific control of polyethylenimine-mediated transient transfection optimized with "Design of experiments" methodology

1 reference

12 December 2020

TubeSpin bioreactor 50 for the high-density cultivation of Sf-9 insect cells in suspension

1 reference

12 December 2020

10.1016/J.PEP.2013.09.001

Identifiers

DOI

1 reference

https://www.ebi.ac.uk/europepmc/webservices/rest/search?query=EXT_ID:24021764%20AND%20SRC:MED&resulttype=core&format=json

13 June 2020

1 reference

https://www.ebi.ac.uk/europepmc/webservices/rest/search?query=EXT_ID:24021764%20AND%20SRC:MED&resulttype=core&format=json

13 June 2020

24021764

1 reference