(Q26780446)

https://www.ebi.ac.uk/europepmc/webservices/rest/search?query=EXT_ID:26492240%20AND%20SRC:MED&resulttype=core&format=json

28 February 2020

review article

0 references

title

High-Throughput Screening in Protein Engineering: Recent Advances and Future Perspectives (English)

1 reference

https://www.ebi.ac.uk/europepmc/webservices/rest/search?query=EXT_ID:26492240%20AND%20SRC:MED&resulttype=core&format=json

28 February 2020

0 references

0 references

high-throughput screening

0 references

biomedical investigative technique

1 reference

19 September 2019

2

1 reference

https://www.ebi.ac.uk/europepmc/webservices/rest/search?query=EXT_ID:26492240%20AND%20SRC:MED&resulttype=core&format=json

28 February 2020

author name string

Magdalena Wójcik

series ordinal

1

1 reference

https://www.ebi.ac.uk/europepmc/webservices/rest/search?query=EXT_ID:26492240%20AND%20SRC:MED&resulttype=core&format=json

28 February 2020

Wim J Quax

series ordinal

3

1 reference

https://www.ebi.ac.uk/europepmc/webservices/rest/search?query=EXT_ID:26492240%20AND%20SRC:MED&resulttype=core&format=json

28 February 2020

Ykelien L Boersma

series ordinal

4

1 reference

https://www.ebi.ac.uk/europepmc/webservices/rest/search?query=EXT_ID:26492240%20AND%20SRC:MED&resulttype=core&format=json

28 February 2020

language of work or name

English

0 references

publication date

20 October 2015

2 references

19 September 2019

https://www.ebi.ac.uk/europepmc/webservices/rest/search?query=EXT_ID:26492240%20AND%20SRC:MED&resulttype=core&format=json

International Journal of Molecular Sciences

28 February 2020

published in

2 references

19 September 2019

https://www.ebi.ac.uk/europepmc/webservices/rest/search?query=EXT_ID:26492240%20AND%20SRC:MED&resulttype=core&format=json

28 February 2020

volume

16

1 reference

https://www.ebi.ac.uk/europepmc/webservices/rest/search?query=EXT_ID:26492240%20AND%20SRC:MED&resulttype=core&format=json

28 February 2020

issue

10

1 reference

https://www.ebi.ac.uk/europepmc/webservices/rest/search?query=EXT_ID:26492240%20AND%20SRC:MED&resulttype=core&format=json

28 February 2020

page(s)

24918-24945

1 reference

https://www.ebi.ac.uk/europepmc/webservices/rest/search?query=EXT_ID:26492240%20AND%20SRC:MED&resulttype=core&format=json

Creative Commons Attribution

28 February 2020

copyright license

1 reference

19 September 2019

0 references

Engineering of TEV protease variants by yeast ER sequestration screening (YESS) of combinatorial libraries

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4632782

Substrate profiling of tobacco etch virus protease using a novel fluorescence-assisted whole-cell assay

7 April 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4632782

Ultrahigh-throughput screening in drop-based microfluidics for directed evolution

7 April 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4632782

A roadmap to directed enzyme evolution and screening systems for biotechnological applications

7 April 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4632782

Accurate microfluidic sorting of droplets at 30 kHz.

7 April 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4632782

Structural basis for the substrate specificity of tobacco etch virus protease

7 April 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4632782

Structure of sortase, the transpeptidase that anchors proteins to the cell wall of Staphylococcus aureus

7 April 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4632782

Structure of staphylococcal alpha-hemolysin, a heptameric transmembrane pore

7 April 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4632782

Tobacco etch virus protease: mechanism of autolysis and rational design of stable mutants with wild-type catalytic proficiency

7 April 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4632782

Conceptual and methodological advances in cell-free directed evolution

7 April 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4632782

Dissecting enzyme function with microfluidic-based deep mutational scanning

28 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4632782

A fluorescent hydrogel-based flow cytometry high-throughput screening platform for hydrolytic enzymes.

28 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4632782

Droplet-based microfluidics platform for ultra-high-throughput bioprospecting of cellulolytic microorganisms

28 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4632782

A high-throughput cellulase screening system based on droplet microfluidics

28 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4632782

Rapid directed evolution of stabilized proteins with cellular high-throughput encapsulation solubilization and screening (CHESS).

28 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4632782

Reprogramming the specificity of sortase enzymes.

28 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4632782

Evolution of enzyme catalysts caged in biomimetic gel-shell beads

28 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4632782

Deep mutational scanning: a new style of protein science

28 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4632782

Liposome display for in vitro selection and evolution of membrane proteins.

28 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4632782

CotA laccase: high-throughput manipulation and analysis of recombinant enzyme libraries expressed in E. coli using droplet-based microfluidics

28 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4632782

Recent advances in rational approaches for enzyme engineering

28 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4632782

One in a million: flow cytometric sorting of single cell-lysate assays in monodisperse picolitre double emulsion droplets for directed evolution

28 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4632782

High-throughput screening for industrial enzyme production hosts by droplet microfluidics.

28 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4632782

New glycosidase substrates for droplet-based microfluidic screening

28 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4632782

Biocatalysis in organic chemistry and biotechnology: past, present, and future

28 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4632782

Capturing the mutational landscape of the beta-lactamase TEM-1.

28 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4632782

Mammalian cell display for the discovery and optimization of antibody therapeutics

28 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4632782

Strategies for the discovery and engineering of enzymes for biocatalysis

28 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4632782

De novo enzymes by computational design

28 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4632782

Direct molecular evolution of detergent-stable G protein-coupled receptors using polymer encapsulated cells

28 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4632782

Flow cytometry-based ultra-high-throughput screening assay for cellulase activity

28 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4632782

Cell-free protein synthesis of membrane (1,3)-β-d-glucan (curdlan) synthase: co-translational insertion in liposomes and reconstitution in nanodiscs

28 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4632782

Vanadium bromoperoxidase-coupled fluorescent assay for flow cytometry sorting of glucose oxidase gene libraries in double emulsions

28 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4632782

Maximizing detergent stability and functional expression of a GPCR by exhaustive recombination and evolution

28 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4632782

Engineering the third wave of biocatalysis

28 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4632782

On-chip continuous monitoring of motile microorganisms on an ePetri platform

28 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4632782

Tolerability, pharmacokinetics and pharmacodynamics of CMAB007, a humanized anti-immunoglobulin E monoclonal antibody, in healthy Chinese subjects

28 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4632782

Self-regulated, droplet-based sample chopper for microfluidic absorbance detection

28 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4632782

Coupling mammalian cell surface display with somatic hypermutation for the discovery and maturation of human antibodies

28 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4632782

Metagenomic cellulases highly tolerant towards the presence of ionic liquids--linking thermostability and halotolerance

28 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4632782

A general strategy for the evolution of bond-forming enzymes using yeast display

28 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4632782

A flow cytometry-based screening system for directed evolution of proteases.

28 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4632782

High-throughput injection with microfluidics using picoinjectors

28 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4632782

Polymer hydrogel capsules: en route toward synthetic cellular systems

28 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4632782

Directed evolution of enzymes: Library screening strategies

28 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4632782

Isolation of human monoclonal antibodies by mammalian cell display

28 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4632782

The future of high-throughput screening

28 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4632782

The biochemistry of somatic hypermutation

28 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4632782

28 September 2017

Droplet microfluidics

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4632782

Yeast surface display for protein engineering and characterization

28 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4632782

Selection strategies for improved biocatalysts

28 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4632782

Cell-free translation systems for protein engineering

28 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4632782

Miniaturising the laboratory in emulsion droplets

28 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4632782

Metagenomics and industrial applications

28 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4632782

SspB delivery of substrates for ClpXP proteolysis probed by the design of improved degradation tags

28 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4632782

In vitro compartmentalization by double emulsions: sorting and gene enrichment by fluorescence activated cell sorting

28 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4632782

Millisecond Kinetics on a Microfluidic Chip Using Nanoliters of Reagents

28 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4632782

Genetic analysis of the twin arginine translocator secretion pathway in bacteria

28 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4632782

The SsrA-SmpB system for protein tagging, directed degradation and ribosome rescue

28 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4632782

Directed evolution of biocatalysts

28 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4632782

A general approach to antibody thermostabilization

28 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4632782

Novel fluorescence-assisted whole-cell assay for engineering and characterization of proteases and their substrates

31 May 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4632782

Man-made cell-like compartments for molecular evolution

31 May 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4632782

Microfluidic large-scale integration

31 May 2018

1 reference

https://api.crossref.org/works/10.3390%2FIJMS161024918

A Competitive Flow Cytometry Screening System for Directed Evolution of Therapeutic Enzyme.

21 January 2018

1 reference

https://api.crossref.org/works/10.3390%2FIJMS161024918

Arginine biosynthesis in Escherichia coli: experimental perturbation and mathematical modeling

21 January 2018

1 reference

https://api.crossref.org/works/10.3390%2FIJMS161024918

Functional analysis of membranous Fo-a subunit of F1Fo-ATP synthase by in vitro protein synthesis.

21 January 2018

1 reference

https://api.crossref.org/works/10.3390%2FIJMS161024918

In vitro membrane protein synthesis inside cell-sized vesicles reveals the dependence of membrane protein integration on vesicle volume.

21 January 2018

1 reference

https://api.crossref.org/works/10.3390%2FIJMS161024918

Biorecognition through layer-by-layer polyelectrolyte assembly: in-situ hybridization on living cells

21 January 2018

1 reference

https://api.crossref.org/works/10.3390%2FIJMS161024918

A flow cytometer-based whole cell screening toolbox for directed hydrolase evolution through fluorescent hydrogels.

21 January 2018

1 reference

https://api.crossref.org/works/10.3390%2FIJMS161024918

Improving the apo-state detergent stability of NTS1 with CHESS for pharmacological and structural studies.

21 January 2018

1 reference

https://api.crossref.org/works/10.3390%2FIJMS161024918

Manipulation of droplets in microfluidic systems

21 January 2018

1 reference

https://api.crossref.org/works/10.3390%2FIJMS161024918

Formation of dispersions using “flow focusing” in microchannels

21 January 2018

1 reference

https://api.crossref.org/works/10.3390%2FIJMS161024918

Monodisperse double emulsions generated from a microcapillary device.

21 January 2018

1 reference

https://api.crossref.org/works/10.3390%2FIJMS161024918

Fluorescence-activated droplet sorting (FADS): efficient microfluidic cell sorting based on enzymatic activity.

21 January 2018

1 reference

https://api.crossref.org/works/10.3390%2FIJMS161024918

Picoliter cell lysate assays in microfluidic droplet compartments for directed enzyme evolution.

21 January 2018

1 reference

https://api.crossref.org/works/10.3390%2FIJMS161024918

A completely in vitro ultrahigh-throughput droplet-based microfluidic screening system for protein engineering and directed evolution.

21 January 2018

1 reference

https://api.crossref.org/works/10.3390%2FIJMS161024918

From microdroplets to microfluidics: selective emulsion separation in microfluidic devices

21 January 2018

1 reference

https://api.crossref.org/works/10.3390%2FIJMS161024918

Ultrafast Surface Enhanced Resonance Raman Scattering Detection in Droplet-Based Microfluidic Systems

21 January 2018

1 reference

https://api.crossref.org/works/10.3390%2FIJMS161024918

Single Living Cell Encapsulation in Nano-organized Polyelectrolyte Shells

21 January 2018

1 reference

https://api.crossref.org/works/10.3390%2FIJMS161024918

Spontaneous transfer of phospholipid-coated oil-in-oil and water-in-oil micro-droplets through an oil/water interface

21 January 2018

1 reference

PubMed

https://pubmed.ncbi.nlm.nih.gov/26492240

inferred from PubMed ID database lookup

12 December 2020

based on heuristic

A simple microfluidic device for fabrication of double emulsion droplets and polymer microcapsules

1 reference

https://api.crossref.org/works/10.3390%2FIJMS161024918

inferred from DOI database lookup

7 January 2021

based on heuristic

LXXIII.—Oxidation of tartaric acid in presence of iron

1 reference

https://api.crossref.org/works/10.3390%2FIJMS161024918

inferred from DOI database lookup

7 January 2021

based on heuristic

Identifiers

DOI

10.3390/IJMS161024918

2 references

https://www.ebi.ac.uk/europepmc/webservices/rest/search?query=EXT_ID:26492240%20AND%20SRC:MED&resulttype=core&format=json

28 February 2020

1 reference

2 references

https://www.ebi.ac.uk/europepmc/webservices/rest/search?query=EXT_ID:26492240%20AND%20SRC:MED&resulttype=core&format=json

28 February 2020

2 references

https://www.ebi.ac.uk/europepmc/webservices/rest/search?query=EXT_ID:26492240%20AND%20SRC:MED&resulttype=core&format=json