(Q33827075)

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Comprehensive analysis of protein digestion using six trypsins reveals the origin of trypsin as a significant source of variability in proteomics

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scholarly article

1 reference

Europe PubMed Central

29 July 2017

Comprehensive analysis of protein digestion using six trypsins reveals the origin of trypsin as a significant source of variability in proteomics (English)

1 reference

Europe PubMed Central

29 July 2017

Scott J. Walmsley

object named as

Scott J Walmsley

1

0 references

Alexey I. Nesvizhskii

object named as

Alexey I Nesvizhskii

6

0 references

author name string

Paul A Rudnick

2

1 reference

Europe PubMed Central

29 July 2017

Yuxue Liang

3

1 reference

Europe PubMed Central

29 July 2017

Qian Dong

4

1 reference

Europe PubMed Central

29 July 2017

Stephen E Stein

5

1 reference

Europe PubMed Central

29 July 2017

publication date

14 November 2013

1 reference

Europe PubMed Central

29 July 2017

Journal of Proteome Research

1 reference

Europe PubMed Central

29 July 2017

12

1 reference

Europe PubMed Central

29 July 2017

12

1 reference

Europe PubMed Central

29 July 2017

5666-5680

1 reference

Europe PubMed Central

29 July 2017

In-source fragmentation and the sources of partially tryptic peptides in shotgun proteomics.

1 reference

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Prediction of missed proteolytic cleavages for the selection of surrogate peptides for quantitative proteomics

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Quantitative mass spectrometry in proteomics: critical review update from 2007 to the present

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Selected reaction monitoring-based proteomics: workflows, potential, pitfalls and future directions

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Targeted data extraction of the MS/MS spectra generated by data-independent acquisition: a new concept for consistent and accurate proteome analysis

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An assessment of the ultrasonic probe-based enhancement of protein cleavage with immobilized trypsin

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Bioanalytical approaches to analyzing peptides and proteins by LC--MS/MS.

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Addressing trypsin bias in large scale (phospho)proteome analysis by size exclusion chromatography and secondary digestion of large post-trypsin peptides.

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Generating and navigating proteome maps using mass spectrometry.

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A quantitative study of the effects of chaotropic agents, surfactants, and solvents on the digestion efficiency of human plasma proteins by trypsin

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Value of using multiple proteases for large-scale mass spectrometry-based proteomics

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Trans-proteomic pipeline: a pipeline for proteomic analysis

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Nanobiocatalysis for protein digestion in proteomic analysis

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Repeatability and reproducibility in proteomic identifications by liquid chromatography-tandem mass spectrometry

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Interlaboratory study characterizing a yeast performance standard for benchmarking LC-MS platform performance.

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Multi-site assessment of the precision and reproducibility of multiple reaction monitoring-based measurements of proteins in plasma

1 reference

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Does trypsin cut before proline?

1 reference

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Effects of modified digestion schemes on the identification of proteins from complex mixtures

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Serine protease mechanism and specificity

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Proteolysis Data Bank: specificity of alpha-chymotrypsin from computation of protein cleavages

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Cutting edge proteomics: benchmarking of six commercial trypsins.

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Quantitation of therapeutic proteins following direct trypsin digestion of dried blood spot samples and detection by LC-MS-based bioanalytical methods in drug discovery

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An improved trypsin digestion method minimizes digestion-induced modifications on proteins.

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Comparison of digestion protocols for microgram quantities of enriched protein samples.

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Trypsin cleaves exclusively C-terminal to arginine and lysine residues

1 reference

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Large-Scale Quantitative Assessment of Different In-Solution Protein Digestion Protocols Reveals Superior Cleavage Efficiency of Tandem Lys-C/Trypsin Proteolysis over Trypsin Digestion

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Systematic and quantitative comparison of digest efficiency and specificity reveals the impact of trypsin quality on MS-based proteomics

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Identifiers

10.1021/PR400611H

1 reference

Europe PubMed Central

29 July 2017

1 reference

Europe PubMed Central

29 July 2017

1 reference

Europe PubMed Central

29 July 2017

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