(Q38354623)

English

Characterization of a cysteine-less human reduced folate carrier: localization of a substrate-binding domain by cysteine-scanning mutagenesis and cysteine accessibility methods

scientific article published on August 15, 2003

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scholarly article

1 reference

Europe PubMed Central

PMC publication ID

2 September 2017

Characterization of a cysteine-less human reduced folate carrier: localization of a substrate-binding domain by cysteine-scanning mutagenesis and cysteine accessibility methods (English)

1 reference

Europe PubMed Central

PMC publication ID

2 September 2017

1 reference

based on heuristic

inferred from title

cell surface receptor

1 reference

Europe PubMed Central

PubMed publication ID

https://www.ebi.ac.uk/europepmc/webservices/rest/search?resulttype=core&format=json&query=SRC%3AMED%20AND%20EXT_ID%3A12749765

2 January 2024

site-directed mutagenesis

1 reference

Europe PubMed Central

PubMed publication ID

https://www.ebi.ac.uk/europepmc/webservices/rest/search?resulttype=core&format=json&query=SRC%3AMED%20AND%20EXT_ID%3A12749765

2 January 2024

1 reference

10.1042/BJ20030301

https://api.crossref.org/works/10.1042/BJ20030301

2 January 2024

1 reference

10.1042/BJ20030301

https://api.crossref.org/works/10.1042/BJ20030301

2 January 2024

author name string

Wei Cao

1

1 reference

Europe PubMed Central

PMC publication ID

2 September 2017

Larry H. Matherly

2

1 reference

Europe PubMed Central

PubMed publication ID

https://www.ebi.ac.uk/europepmc/webservices/rest/search?resulttype=core&format=json&query=SRC%3AMED%20AND%20EXT_ID%3A12749765

2 January 2024

language of work or name

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publication date

1 August 2003

1 reference

Europe PubMed Central

PMC publication ID

2 September 2017

15 August 2003

1 reference

National Center for Biotechnology Information

PubMed publication ID

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/esummary.fcgi?db=pubmed&retmode=json&id=12749765

2 January 2024

full work available at URL

https://europepmc.org/articles/pmc1223575?pdf=render

Portable Document Format

online access status

1 reference

Europe PubMed Central

PubMed publication ID

https://www.ebi.ac.uk/europepmc/webservices/rest/search?resulttype=core&format=json&query=SRC%3AMED%20AND%20EXT_ID%3A12749765

2 January 2024

https://europepmc.org/articles/PMC1223575

online access status

content deliverer

Europe PubMed Central

1 reference

Europe PubMed Central

PubMed publication ID

https://www.ebi.ac.uk/europepmc/webservices/rest/search?resulttype=core&format=json&query=SRC%3AMED%20AND%20EXT_ID%3A12749765

2 January 2024

https://europepmc.org/articles/PMC1223575?pdf=render

Portable Document Format

online access status

content deliverer

Europe PubMed Central

1 reference

Europe PubMed Central

PubMed publication ID

https://www.ebi.ac.uk/europepmc/webservices/rest/search?resulttype=core&format=json&query=SRC%3AMED%20AND%20EXT_ID%3A12749765

2 January 2024

https://doi.org/10.1042/bj20030301

online access status

1 reference

Europe PubMed Central

PubMed publication ID

https://www.ebi.ac.uk/europepmc/webservices/rest/search?resulttype=core&format=json&query=SRC%3AMED%20AND%20EXT_ID%3A12749765

2 January 2024

https://portlandpress.com/biochemj/article-pdf/374/1/27/711977/bj3740027.pdf

Portable Document Format

1 reference

10.1042/BJ20030301

https://api.crossref.org/works/10.1042/BJ20030301

2 January 2024

Biochemical Journal

1 reference

Europe PubMed Central

PMC publication ID

2 September 2017

374

1 reference

Europe PubMed Central

PMC publication ID

2 September 2017

Pt 1

1 reference

Europe PubMed Central

PMC publication ID

2 September 2017

27-36

1 reference

Europe PubMed Central

PMC publication ID

2 September 2017

Protein measurement with the Folin phenol reagent

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1223575

6 September 2017

Identification of lysine-411 in the human reduced folate carrier as an important determinant of substrate selectivity and carrier function by systematic site-directed mutagenesis

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1223575

6 September 2017

Analysis of membrane topology of the human reduced folate carrier protein by hemagglutinin epitope insertion and scanning glycosylation insertion mutagenesis

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1223575

6 September 2017

The human reduced folate carrier gene is ubiquitously and differentially expressed in normal human tissues: identification of seven non-coding exons and characterization of a novel promoter

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1223575

6 September 2017

Resistance to multiple novel antifolates is mediated via defective drug transport resulting from clustered mutations in the reduced folate carrier gene in human leukaemia cell lines

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1223575

6 September 2017

Molecular and cellular biology of the human reduced folate carrier

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1223575

6 September 2017

Site-directed sulfhydryl labeling of the lactose permease of Escherichia coli: helices IV and V that contain the major determinants for substrate binding

1 reference

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6 September 2017

Functional interactions between arginine-133 and aspartate-88 in the human reduced folate carrier: evidence for a charge-pair association

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1223575

6 September 2017

Helix packing of functionally important regions of the cardiac Na(+)-Ca(2+) exchanger

1 reference

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6 September 2017

Site-directed chemical labeling of extracellular loops in a membrane protein. The topology of the Na,K-ATPase alpha-subunit

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1223575

6 September 2017

NPS@: network protein sequence analysis

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1223575

6 September 2017

Determining the structure and mechanism of the human multidrug resistance P-glycoprotein using cysteine-scanning mutagenesis and thiol-modification techniques

1 reference

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6 September 2017

Mechanisms of methotrexate resistance in acute leukemia. Decreased transport and polyglutamylation

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1223575

6 September 2017

Carrier-mediated membrane transport of folates in mammalian cells

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1223575

6 September 2017

Topological and functional analysis of the human reduced folate carrier by hemagglutinin epitope insertion

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1223575

6 September 2017

Cys-scanning mutagenesis: a novel approach to structure function relationships in polytopic membrane proteins

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1223575

6 September 2017

Cocaine alters the accessibility of endogenous cysteines in putative extracellular and intracellular loops of the human dopamine transporter.

1 reference

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6 September 2017

Homocysteine and cardiovascular disease

1 reference

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6 September 2017

A cysteine residue in the third membrane-spanning segment of the human D2 dopamine receptor is exposed in the binding-site crevice.

1 reference

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6 September 2017

Isolation of a human cDNA that complements a mutant hamster cell defective in methotrexate uptake

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1223575

6 September 2017

Molecular cloning of the human placental folate transporter

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1223575

6 September 2017

Isolation of a gene encoding a human reduced folate carrier (RFC1) and analysis of its expression in transport-deficient, methotrexate-resistant human breast cancer cells.

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1223575

6 September 2017

Isolation of human cDNAs that restore methotrexate sensitivity and reduced folate carrier activity in methotrexate transport-defective Chinese hamster ovary cells

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1223575

6 September 2017

Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1223575

6 September 2017

Sequence from picomole quantities of proteins electroblotted onto polyvinylidene difluoride membranes

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1223575

6 September 2017

The cellular pharmacology of methotrexate

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1223575

6 September 2017

Gene splicing by overlap extension: tailor-made genes using the polymerase chain reaction

1 reference

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6 September 2017

Acetylcholine receptor channel structure probed in cysteine-substitution mutants.

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1223575

6 September 2017

Role of the E45K-reduced folate carrier gene mutation in methotrexate resistance in human leukemia cells.

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1223575

14 September 2017

Obligate genetic expression in tumor cells of a fetal membrane property mediating "folate" transport: biological significance and implications for improved therapy of human cancer

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1223575

14 September 2017

Functional role of arginine 373 in substrate translocation by the reduced folate carrier

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1223575

27 September 2017

Cysteine 144 in the third transmembrane domain of the creatine transporter is located close to a substrate-binding site

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1223575

27 September 2017

A reduced folate carrier mutation produces substrate-dependent alterations in carrier mobility in murine leukemia cells and methotrexate resistance with conservation of growth in 5-formyltetrahydrofolate

1 reference

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27 September 2017

Folic acid and the prevention of birth defects

1 reference

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27 September 2017

Folate: effects on carcinogenesis and the potential for cancer chemoprevention.

1 reference

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27 September 2017

Membrane topology of a cysteine-less mutant of human P-glycoprotein

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1223575

27 September 2017

A mutated murine reduced folate carrier (RFC1) with increased affinity for folic acid, decreased affinity for methotrexate, and an obligatory anion requirement for transport function.

1 reference

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23 June 2018

Identification of a highly glycosylated methotrexate membrane carrier in K562 human erythroleukemia cells up-regulated for tetrahydrofolate cofactor and methotrexate transport.

1 reference

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23 June 2018

Isolation and partial characterization of three methotrexate-resistant phenotypes from Chinese hamster ovary cells

1 reference

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23 June 2018

Sequence alterations in the reduced folate carrier are observed in osteosarcoma tumor samples.

1 reference

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31 August 2018

The mitochondrial oxoglutarate carrier: cysteine-scanning mutagenesis of transmembrane domain IV and sensitivity of Cys mutants to sulfhydryl reagents

1 reference

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Analysis of transmembrane segment 10 of the Glut1 glucose transporter by cysteine-scanning mutagenesis and substituted cysteine accessibility

1 reference

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Determining the dimensions of the drug-binding domain of human P-glycoprotein using thiol cross-linking compounds as molecular rulers

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1223575

31 August 2018

Mutational analysis of the functional role of conserved arginine and lysine residues in transmembrane domains of the murine reduced folate carrier

1 reference

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31 August 2018

Defining the drug-binding site in the human multidrug resistance P-glycoprotein using a methanethiosulfonate analog of verapamil, MTS-verapamil

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31 August 2018

Complete cysteine-scanning mutagenesis and site-directed chemical modification of the Tn10-encoded metal-tetracycline/H+ antiporter

1 reference

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31 August 2018

Cysteine-scanning mutagenesis reveals a highly amphipathic, pore-lining membrane-spanning helix in the glutamate transporter GltT.

1 reference

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31 August 2018

Clustering of mutations in the first transmembrane domain of the human reduced folate carrier in GW1843U89-resistant leukemia cells with impaired antifolate transport and augmented folate uptake.

1 reference

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31 August 2018

Cysteine scanning mutagenesis of helices 2 and 7 in GLUT1 identifies an exofacial cleft in both transmembrane segments.

1 reference

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31 August 2018

Mapping the substrate binding site of the prostaglandin transporter PGT by cysteine scanning mutagenesis.

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31 August 2018

Impaired membrane transport in methotrexate-resistant CCRF-CEM cells involves early translation termination and increased turnover of a mutant reduced folate carrier.

1 reference

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31 August 2018

A new topological model of the cardiac sarcolemmal Na+-Ca2+ exchanger

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31 August 2018

A structurally altered human reduced folate carrier with increased folic acid transport mediates a novel mechanism of antifolate resistance.

1 reference

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31 August 2018

Mutations in the reduced folate carrier gene which confer dominant resistance to 5,10-dideazatetrahydrofolate

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31 August 2018

Identification of Residues within the Drug-binding Domain of the Human Multidrug Resistance P-glycoprotein by Cysteine-scanning Mutagenesis and Reaction with Dibromobimane

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3 December 2018

Carrier-mediated Transport of the Folic Acid Analogue, Methotrexate, in the L1210 Leukemia Cell

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12 December 2020

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Rapid formation of poly-gamma-glutamyl derivatives of methotrexate and their association with dihydrofolate reductase as assessed by high pressure liquid chromatography in the Ehrlich ascites tumor cell in vitro

1 reference

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12 December 2020

based on heuristic

inferred from PubMed ID database lookup

Human K562 transfectants expressing high levels of reduced folate carrier but exhibiting low transport activity

1 reference

https://pubmed.ncbi.nlm.nih.gov/12749765

12 December 2020

based on heuristic

inferred from PubMed ID database lookup

Substituted-cysteine accessibility method

1 reference

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inferred from PubMed ID database lookup

Reduced folate carrier gene expression in childhood acute lymphoblastic leukemia: relationship to immunophenotype and ploidy

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12 December 2020

based on heuristic

inferred from PubMed ID database lookup

Mechanisms of methotrexate resistance in osteosarcoma

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12 December 2020

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Use of a disulfide cross-linking strategy to study muscarinic receptor structure and mechanisms of activation

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12 December 2020

based on heuristic

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Role of the amino acid 45 residue in reduced folate carrier function and ion-dependent transport as characterized by site-directed mutagenesis

1 reference

https://pubmed.ncbi.nlm.nih.gov/12749765

12 December 2020

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Cysteine-scanning mutagenesis around transmembrane segments 1 and 11 and their flanking loop regions of Tn10-encoded metal-Tetracycline/H+ antiporter

1 reference

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12 December 2020

based on heuristic

inferred from PubMed ID database lookup

Identifiers

10.1042/BJ20030301

1 reference

Europe PubMed Central

PMC publication ID

2 September 2017

PMC publication ID

1 reference

Europe PubMed Central

PMC publication ID

2 September 2017

PubMed publication ID

1 reference

Europe PubMed Central

PMC publication ID

2 September 2017

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