(Q54381690)

29 May 2018

title

Directed In Vitro Evolution of Reporter Genes Based on Semi-Rational Design and High-Throughput Screening (English)

1 reference

https://api.crossref.org/works/10.1007/978-1-60761-652-8_18

14 June 2024

1 reference

https://www.ebi.ac.uk/europepmc/webservices/rest/search?resulttype=core&format=json&query=SRC%3AMED%20AND%20EXT_ID%3A20676989

14 June 2024

reporter genes

1 reference

https://www.ebi.ac.uk/europepmc/webservices/rest/search?resulttype=core&format=json&query=SRC%3AMED%20AND%20EXT_ID%3A20676989

14 June 2024

1

Ai-Sheng Xiong

1 reference

https://www.ebi.ac.uk/europepmc/webservices/rest/search?resulttype=core&format=json&query=SRC%3AMED%20AND%20EXT_ID%3A20676989

14 June 2024

author name string

Quan-Hong Yao

2

1 reference

29 May 2018

Ri-He Peng

3

1 reference

29 May 2018

Zong-Ming Cheng

4

1 reference

29 May 2018

language of work or name

English

0 references

publication date

1 January 2010

1 reference

29 May 2018

full work available at URL

http://link.springer.com/content/pdf/10.1007/978-1-60761-652-8_18

1 reference

https://api.crossref.org/works/10.1007/978-1-60761-652-8_18

Methods in Molecular Biology

14 June 2024

published in

1 reference

29 May 2018

volume

634

1 reference

29 May 2018

page(s)

239-256

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29 May 2018

Rapid evolution of a protein in vitro by DNA shuffling

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Forced Evolution of a Herbicide Detoxifying Glutathione Transferase

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Discovery and directed evolution of a glyphosate tolerance gene

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Metabolic engineering and directed evolution for the production of pharmaceuticals

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DNA shuffling and screening strategies for improving vaccine efficacy

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DNA shuffling of a family of genes from diverse species accelerates directed evolution

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Genome shuffling leads to rapid phenotypic improvement in bacteria

14 June 2024

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One-step random mutagenesis by error-prone rolling circle amplification

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An effective family shuffling method using single-stranded DNA

14 June 2024

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Degenerate oligonucleotide gene shuffling (DOGS): a method for enhancing the frequency of recombination with family shuffling

14 June 2024

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Random insertion and deletion of arbitrary number of bases for codon-based random mutation of DNAs

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Protein evolution by codon-based random deletions

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Directed evolution of polymerase function by compartmentalized self-replication

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Degenerate oligonucleotide gene shuffling (DOGS) and random drift mutagenesis (RNDM): two complementary techniques for enzyme evolution

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Construction of DNA-shuffled and incrementally truncated libraries by a mutagenic and unidirectional reassembly method: changing from a substrate specificity of phospholipase to that of lipase

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Family shuffling of a targeted bphA region to engineer biphenyl dioxygenase.

14 June 2024

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A semi-rational design strategy of directed evolution combined with chemical synthesis of DNA sequences

14 June 2024

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14 June 2024

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Site-saturation mutagenesis is more efficient than DNA shuffling for the directed evolution of beta-fucosidase from beta-galactosidase

14 June 2024

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Selectable marker genes in transgenic plants: applications, alternatives and biosafety

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Directed evolution of a fucosidase from a galactosidase by DNA shuffling and screening

14 June 2024

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Improved green fluorescent protein by molecular evolution using DNA shuffling

14 June 2024

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DNA shuffling brightens prospects for GFP.

14 June 2024

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Functional tuning of a salvaged green fluorescent protein variant with a new sequence space by directed evolution

14 June 2024

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Novel fluorescent protein from Discosoma coral and its mutants possesses a unique far-red fluorescence

14 June 2024

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In vitro evolution of beta-glucuronidase into a beta-galactosidase proceeds through non-specific intermediates

14 June 2024

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Increasing the thermal stability of an oligomeric protein, beta-glucuronidase.

14 June 2024

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High efficiency and throughput system in directed evolution in vitro of reporter gene.

14 June 2024

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Concurrent mutations in six amino acids in beta-glucuronidase improve its thermostability.

14 June 2024

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https://api.crossref.org/works/10.1007/978-1-60761-652-8_18

Directed evolution of the surface chemistry of the reporter enzyme beta-glucuronidase

14 June 2024

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A simple, rapid, high-fidelity and cost-effective PCR-based two-step DNA synthesis method for long gene sequences

14 June 2024

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14 June 2024

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14 June 2024

Identifiers

1 reference

29 May 2018

1 reference

29 May 2018