(Q34018164)

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Enhancing the thermal robustness of an enzyme by directed evolution: least favorable starting points and inferior mutants can map superior evolutionary pathways

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scholarly article

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Europe PubMed Central

30 July 2017

Enhancing the thermal robustness of an enzyme by directed evolution: least favorable starting points and inferior mutants can map superior evolutionary pathways (English)

1 reference

Europe PubMed Central

30 July 2017

directed evolution

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Manfred T. Reetz

2

object named as

Manfred T Reetz

1 reference

Europe PubMed Central

30 July 2017

author name string

Yosephine Gumulya

1

1 reference

Europe PubMed Central

30 July 2017

publication date

13 September 2011

1 reference

Europe PubMed Central

30 July 2017

1 reference

Europe PubMed Central

30 July 2017

12

1 reference

Europe PubMed Central

30 July 2017

2502-2510

1 reference

Europe PubMed Central

30 July 2017

16

1 reference

Europe PubMed Central

30 July 2017

Rational engineering of enzyme stability

1 reference

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21 January 2018

Temperature adaptation of enzymes: lessons from laboratory evolution

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Directed evolution of enzyme stability

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21 January 2018

Proside: a phage-based method for selecting thermostable proteins.

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21 January 2018

Construction of stabilized proteins by combinatorial consensus mutagenesis.

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21 January 2018

Semi-rational approaches to engineering enzyme activity: combining the benefits of directed evolution and rational design

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21 January 2018

Expression and stabilization of galactose oxidase in Escherichia coli by directed evolution

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Functional expression and stabilization of horseradish peroxidase by directed evolution in Saccharomyces cerevisiae

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A thermostable variant of fructose bisphosphate aldolase constructed by directed evolution also shows increased stability in organic solvents

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21 January 2018

Serial increase in the thermal stability of 3-isopropylmalate dehydrogenase from Bacillus subtilis by experimental evolution

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21 January 2018

Directed evolution converts subtilisin E into a functional equivalent of thermitase

1 reference

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21 January 2018

Improvement of oxidative and thermostability of N-carbamyl-d-amino Acid amidohydrolase by directed evolution

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21 January 2018

Molecular dynamics simulations as a tool for improving protein stability

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21 January 2018

Extreme stabilization of a thermolysin-like protease by an engineered disulfide bond

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The effect of proline insertions on the thermostability of a barley alpha-glucosidase

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Engineering of multiple arginines into the Ser/Thr surface of Trichoderma reesei endo-1,4-beta-xylanase II increases the thermotolerance and shifts the pH optimum towards alkaline pH.

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Directed evolution of a thermostable phosphite dehydrogenase for NAD(P)H regeneration

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Directed evolution of the thermostable xylanase from Thermomyces lanuginosus

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Enzymatic analysis of a thermostabilized mutant of an Escherichia coli hygromycin B phosphotransferase.

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Enhancement of thermostability of fungal deglycating enzymes by directed evolution.

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Optimizing protein stability in vivo

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Thermostabilization of an esterase by alignment-guided focussed directed evolution

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Status of protein engineering for biocatalysts: how to design an industrially useful biocatalyst

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21 January 2018

Evolving thermostability in mutant libraries of ligninolytic oxidoreductases expressed in yeast

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21 January 2018

Thermostabilization of Bacillus circulans xylanase: computational optimization of unstable residues based on thermal fluctuation analysis

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21 January 2018

Novel methods for directed evolution of enzymes: quality, not quantity

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21 January 2018

Site-saturation mutagenesis is more efficient than DNA shuffling for the directed evolution of beta-fucosidase from beta-galactosidase

1 reference

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21 January 2018

Analysis of shuffled gene libraries

1 reference

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Mutant library construction in directed molecular evolution: casting a wider net.

1 reference

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21 January 2018

Directed evolution of enzymes and biosynthetic pathways

1 reference

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Directed evolution drives the next generation of biocatalysts

1 reference

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21 January 2018

Protein Design by Directed Evolution

1 reference

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Advances in laboratory evolution of enzymes

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21 January 2018

Exploring protein fitness landscapes by directed evolution

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21 January 2018

Enzyme engineering for enantioselectivity: from trial-and-error to rational design?

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21 January 2018

Advances in generating functional diversity for directed protein evolution.

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Selection strategies for improved biocatalysts

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Shedding light on the efficacy of laboratory evolution based on iterative saturation mutagenesis

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Laboratory evolution of stereoselective enzymes: a prolific source of catalysts for asymmetric reactions

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21 January 2018

Iterative saturation mutagenesis (ISM) for rapid directed evolution of functional enzymes

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21 January 2018

Structural basis of selection and thermostability of laboratory evolved Bacillus subtilis lipase

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21 January 2018

Thermally denatured state determines refolding in lipase: mutational analysis

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21 January 2018

The adrenaline test for enzymes

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Colorimetric assays for quantitative analysis and screening of epoxide hydrolase activity

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A cell-based adrenaline assay for automated high-throughput activity screening of epoxide hydrolases

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Structure of Aspergillus niger epoxide hydrolase at 1.8 A resolution: implications for the structure and function of the mammalian microsomal class of epoxide hydrolases

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Cassette mutagenesis: an efficient method for generation of multiple mutations at defined sites

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Promoters selected from random DNA sequences

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Cloning of random-sequence oligodeoxynucleotides

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21 January 2018

Combinatorial cassette mutagenesis as a probe of the informational content of protein sequences

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Substrate specificity of trypsin investigated by using a genetic selection

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Sequence requirements for coiled-coils: analysis with lambda repressor-GCN4 leucine zipper fusions

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Protein design by binary patterning of polar and nonpolar amino acids

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Codon cassette mutagenesis: a general method to insert or replace individual codons by using universal mutagenic cassettes

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Protein engineers turned evolutionists

1 reference

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The subtle benefits of being promiscuous: adaptive evolution potentiated by enzyme promiscuity

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In the light of directed evolution: pathways of adaptive protein evolution

1 reference

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21 January 2018

Molecular quasi-species

1 reference

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Functionally diverging molecular quasi-species evolve by crossing two enzymes

1 reference

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Identification of emerging quasi-species in directed enzyme evolution

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The quest for molecular quasi-species in ligand-activity space and its application to directed enzyme evolution

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A novel quasi-species of glutathione transferase with high activity towards naturally occurring isothiocyanates evolves from promiscuous low-activity variants

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Contribution of salt bridges toward protein thermostability

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The stability of salt bridges at high temperatures: implications for hyperthermophilic proteins

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Protein thermostability above 100 degreesC: a key role for ionic interactions

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Engineering activity and stability of Thermotoga maritima glutamate dehydrogenase. II: construction of a 16-residue ion-pair network at the subunit interface

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Electrostatic contributions to the stability of hyperthermophilic proteins.

1 reference

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Contribution of surface salt bridges to protein stability: guidelines for protein engineering.

1 reference

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An electrostatic basis for the stability of thermophilic proteins

1 reference

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Contribution of electrostatic interactions, compactness and quaternary structure to protein thermostability: lessons from structural genomics of Thermotoga maritima.

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Structural basis of thermostability. Analysis of stabilizing mutations in subtilisin BPN'

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Engineering monolignol 4-O-methyltransferases to modulate lignin biosynthesis

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Iterative saturation mutagenesis accelerates laboratory evolution of enzyme stereoselectivity: rigorous comparison with traditional methods.

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Structure-guided directed evolution of alkenyl and arylmalonate decarboxylases

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Creating lactose phosphorylase enzymes by directed evolution of cellobiose phosphorylase

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Strategies and computational tools for improving randomized protein libraries

1 reference

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Mathematical expressions useful in the construction, description and evaluation of protein libraries.

1 reference

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Combinatorial manipulation of three key active site residues in glycinamide ribonucleotide transformylase

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Enhancement of the activity of l-aspartase from Escherichia coli W by directed evolution

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Further improvement of phosphite dehydrogenase thermostability by saturation mutagenesis

1 reference

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21 January 2018

Directed evolution of enantioselective enzymes: iterative cycles of CASTing for probing protein-sequence space

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Iterative saturation mutagenesis on the basis of B factors as a strategy for increasing protein thermostability

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Directed Evolution of an Enantioselective Enzyme through Combinatorial Multiple-Cassette Mutagenesis

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Asymmetric Organic Synthesis with Enzymes

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Stabilization of Escherichia coli uridine phosphorylase by evolution and immobilization

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Regio- and stereoselectivity of P450-catalysed hydroxylation of steroids controlled by laboratory evolution

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Inverting the enantioselectivity of P450pyr monooxygenase by directed evolution

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Directed evolution of an enantioselective lipase with broad substrate scope for hydrolysis of alpha-substituted esters

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Identifiers

10.1002/CBIC.201100412

1 reference

Europe PubMed Central

30 July 2017

1 reference

Europe PubMed Central

30 July 2017

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