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CRISPR-Cas9 Structures and Mechanisms

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Europe PubMed Central

10 September 2017

CRISPR-Cas9 Structures and Mechanisms (English)

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Europe PubMed Central

10 September 2017

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Jennifer A Doudna

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Europe PubMed Central

10 September 2017

Fuguo Jiang

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Europe PubMed Central

10 September 2017

30 March 2017

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Europe PubMed Central

10 September 2017

Annual Review of Biophysics

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Europe PubMed Central

10 September 2017

46

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Europe PubMed Central

10 September 2017

505-529

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Europe PubMed Central

10 September 2017

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Clustered regularly interspaced short palindrome repeats (CRISPRs) have spacers of extrachromosomal origin

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Protospacer adjacent motif (PAM)-distal sequences engage CRISPR Cas9 DNA target cleavage.

1 reference

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The Neisseria meningitidis CRISPR-Cas9 System Enables Specific Genome Editing in Mammalian Cells

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RNA-guided RNA cleavage by a CRISPR RNA-Cas protein complex

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CRISPR-Cas9 nuclear dynamics and target recognition in living cells.

1 reference

9 October 2022

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Genome-editing Technologies for Gene and Cell Therapy

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RNA-guided human genome engineering via Cas9

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9 October 2022

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Defining and improving the genome-wide specificities of CRISPR-Cas9 nucleases

1 reference

9 October 2022

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Applications of CRISPR-Cas systems in neuroscience

1 reference

9 October 2022

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Double nicking by RNA-guided CRISPR Cas9 for enhanced genome editing specificity

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9 October 2022

https://opencitations.net/index/coci/api/v1/references/10.1146/ANNUREV-BIOPHYS-062215-010822

Structure and Engineering of Francisella novicida Cas9.

1 reference

9 October 2022

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Discovery and Functional Characterization of Diverse Class 2 CRISPR-Cas Systems

1 reference

9 October 2022

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RNA-guided genetic silencing systems in bacteria and archaea

1 reference

9 October 2022

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Crystal structure of a bacterial RNA polymerase holoenzyme at 2.6 A resolution

1 reference

9 October 2022

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RNA-programmed genome editing in human cells

1 reference

9 October 2022

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Real-time observation of DNA recognition and rejection by the RNA-guided endonuclease Cas9.

1 reference

9 October 2022

https://opencitations.net/index/coci/api/v1/references/10.1146/ANNUREV-BIOPHYS-062215-010822

Phage response to CRISPR-encoded resistance in Streptococcus thermophilus

1 reference

9 October 2022

https://opencitations.net/index/coci/api/v1/references/10.1146/ANNUREV-BIOPHYS-062215-010822

DNA targeting specificity of RNA-guided Cas9 nucleases

1 reference

9 October 2022

https://opencitations.net/index/coci/api/v1/references/10.1146/ANNUREV-BIOPHYS-062215-010822

Nucleosomes Inhibit Cas9 Endonuclease Activity in Vitro.

1 reference

9 October 2022

https://opencitations.net/index/coci/api/v1/references/10.1146/ANNUREV-BIOPHYS-062215-010822

Programmable RNA recognition and cleavage by CRISPR/Cas9.

1 reference

9 October 2022

https://opencitations.net/index/coci/api/v1/references/10.1146/ANNUREV-BIOPHYS-062215-010822

CRISPR/Cas9 for Human Genome Engineering and Disease Research.

1 reference

9 October 2022

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Crystal Structure of Cas9 in Complex with Guide RNA and Target DNA

1 reference

9 October 2022

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Structural Basis for the Altered PAM Specificities of Engineered CRISPR-Cas9

1 reference

9 October 2022

https://opencitations.net/index/coci/api/v1/references/10.1146/ANNUREV-BIOPHYS-062215-010822

Therapeutic genome editing: prospects and challenges

1 reference

9 October 2022

https://opencitations.net/index/coci/api/v1/references/10.1146/ANNUREV-BIOPHYS-062215-010822

CRISPR Provides Acquired Resistance Against Viruses in Prokaryotes

1 reference

9 October 2022

https://opencitations.net/index/coci/api/v1/references/10.1146/ANNUREV-BIOPHYS-062215-010822

Origins of specificity in protein-DNA recognition.

1 reference

9 October 2022

https://opencitations.net/index/coci/api/v1/references/10.1146/ANNUREV-BIOPHYS-062215-010822

Structural basis for promiscuous PAM recognition in type I-E Cascade from E. coli

1 reference

9 October 2022

https://opencitations.net/index/coci/api/v1/references/10.1146/ANNUREV-BIOPHYS-062215-010822

Conformational control of DNA target cleavage by CRISPR-Cas9.

1 reference

9 October 2022

https://opencitations.net/index/coci/api/v1/references/10.1146/ANNUREV-BIOPHYS-062215-010822

In vivo genome editing using Staphylococcus aureus Cas9

1 reference

9 October 2022

https://opencitations.net/index/coci/api/v1/references/10.1146/ANNUREV-BIOPHYS-062215-010822

Genome-wide analysis reveals characteristics of off-target sites bound by the Cas9 endonuclease.

1 reference

9 October 2022

https://opencitations.net/index/coci/api/v1/references/10.1146/ANNUREV-BIOPHYS-062215-010822

CRISPR-Cas immunity in prokaryotes

1 reference

9 October 2022

https://opencitations.net/index/coci/api/v1/references/10.1146/ANNUREV-BIOPHYS-062215-010822

Structural Plasticity of PAM Recognition by Engineered Variants of the RNA-Guided Endonuclease Cas9.

1 reference

9 October 2022

https://opencitations.net/index/coci/api/v1/references/10.1146/ANNUREV-BIOPHYS-062215-010822

Classification and evolution of type II CRISPR-Cas systems

1 reference

9 October 2022

https://opencitations.net/index/coci/api/v1/references/10.1146/ANNUREV-BIOPHYS-062215-010822

Direct observation of R-loop formation by single RNA-guided Cas9 and Cascade effector complexes.

1 reference

9 October 2022

https://opencitations.net/index/coci/api/v1/references/10.1146/ANNUREV-BIOPHYS-062215-010822

Structural basis of PAM-dependent target DNA recognition by the Cas9 endonuclease

1 reference

9 October 2022

https://opencitations.net/index/coci/api/v1/references/10.1146/ANNUREV-BIOPHYS-062215-010822

Crystal structure of the RNA-guided immune surveillance Cascade complex in Escherichia coli

1 reference

9 October 2022

https://opencitations.net/index/coci/api/v1/references/10.1146/ANNUREV-BIOPHYS-062215-010822

High-frequency off-target mutagenesis induced by CRISPR-Cas nucleases in human cells

1 reference

9 October 2022

https://opencitations.net/index/coci/api/v1/references/10.1146/ANNUREV-BIOPHYS-062215-010822

Self versus non-self discrimination during CRISPR RNA-directed immunity.

1 reference

9 October 2022

https://opencitations.net/index/coci/api/v1/references/10.1146/ANNUREV-BIOPHYS-062215-010822

Structures of a CRISPR-Cas9 R-loop complex primed for DNA cleavage

1 reference

9 October 2022

https://opencitations.net/index/coci/api/v1/references/10.1146/ANNUREV-BIOPHYS-062215-010822

An updated evolutionary classification of CRISPR-Cas systems

1 reference

9 October 2022

https://opencitations.net/index/coci/api/v1/references/10.1146/ANNUREV-BIOPHYS-062215-010822

CRISPR interference limits horizontal gene transfer in staphylococci by targeting DNA

1 reference

9 October 2022

https://opencitations.net/index/coci/api/v1/references/10.1146/ANNUREV-BIOPHYS-062215-010822

Nucleosome breathing and remodeling constrain CRISPR-Cas9 function

1 reference

9 October 2022

https://opencitations.net/index/coci/api/v1/references/10.1146/ANNUREV-BIOPHYS-062215-010822

Orthogonal Cas9 proteins for RNA-guided gene regulation and editing

1 reference

9 October 2022

https://opencitations.net/index/coci/api/v1/references/10.1146/ANNUREV-BIOPHYS-062215-010822

The CRISPR/Cas bacterial immune system cleaves bacteriophage and plasmid DNA

1 reference

9 October 2022

https://opencitations.net/index/coci/api/v1/references/10.1146/ANNUREV-BIOPHYS-062215-010822

CRISPR RNA maturation by trans-encoded small RNA and host factor RNase III

1 reference

9 October 2022

https://opencitations.net/index/coci/api/v1/references/10.1146/ANNUREV-BIOPHYS-062215-010822

Genome editing: the end of the beginning

1 reference

9 October 2022

https://opencitations.net/index/coci/api/v1/references/10.1146/ANNUREV-BIOPHYS-062215-010822

Unravelling the structural and mechanistic basis of CRISPR-Cas systems

1 reference

9 October 2022

https://opencitations.net/index/coci/api/v1/references/10.1146/ANNUREV-BIOPHYS-062215-010822

Efficient genome engineering in human pluripotent stem cells using Cas9 from Neisseria meningitidis

1 reference

9 October 2022

https://opencitations.net/index/coci/api/v1/references/10.1146/ANNUREV-BIOPHYS-062215-010822

Development and applications of CRISPR-Cas9 for genome engineering

1 reference

9 October 2022

https://opencitations.net/index/coci/api/v1/references/10.1146/ANNUREV-BIOPHYS-062215-010822

Origins of Programmable Nucleases for Genome Engineering

1 reference

9 October 2022

https://opencitations.net/index/coci/api/v1/references/10.1146/ANNUREV-BIOPHYS-062215-010822

Biotechnology: Rewriting a genome

1 reference

9 October 2022

https://opencitations.net/index/coci/api/v1/references/10.1146/ANNUREV-BIOPHYS-062215-010822

Striking Plasticity of CRISPR-Cas9 and Key Role of Non-target DNA, as Revealed by Molecular Simulations.

1 reference

9 October 2022

https://opencitations.net/index/coci/api/v1/references/10.1146/ANNUREV-BIOPHYS-062215-010822

Phylogeny of Cas9 determines functional exchangeability of dual-RNA and Cas9 among orthologous type II CRISPR-Cas systems

1 reference

9 October 2022

https://opencitations.net/index/coci/api/v1/references/10.1146/ANNUREV-BIOPHYS-062215-010822

Chemical Biology Approaches to Genome Editing: Understanding, Controlling, and Delivering Programmable Nucleases

1 reference

9 October 2022

https://opencitations.net/index/coci/api/v1/references/10.1146/ANNUREV-BIOPHYS-062215-010822

Diversity, activity, and evolution of CRISPR loci in Streptococcus thermophilus

1 reference

9 October 2022

https://opencitations.net/index/coci/api/v1/references/10.1146/ANNUREV-BIOPHYS-062215-010822

CRISPR-Cas systems for editing, regulating and targeting genomes

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9 October 2022

https://opencitations.net/index/coci/api/v1/references/10.1146/ANNUREV-BIOPHYS-062215-010822

Rationally engineered Cas9 nucleases with improved specificity

1 reference

9 October 2022

https://opencitations.net/index/coci/api/v1/references/10.1146/ANNUREV-BIOPHYS-062215-010822

Biology and Applications of CRISPR Systems: Harnessing Nature's Toolbox for Genome Engineering

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9 October 2022

https://opencitations.net/index/coci/api/v1/references/10.1146/ANNUREV-BIOPHYS-062215-010822

Adapting to new threats: the generation of memory by CRISPR-Cas immune systems

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9 October 2022

https://opencitations.net/index/coci/api/v1/references/10.1146/ANNUREV-BIOPHYS-062215-010822

Applications of CRISPR technologies in research and beyond

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9 October 2022

https://opencitations.net/index/coci/api/v1/references/10.1146/ANNUREV-BIOPHYS-062215-010822

High-throughput profiling of off-target DNA cleavage reveals RNA-programmed Cas9 nuclease specificity

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9 October 2022

https://opencitations.net/index/coci/api/v1/references/10.1146/ANNUREV-BIOPHYS-062215-010822

CRISPR/Cas9 in Genome Editing and Beyond

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9 October 2022

https://opencitations.net/index/coci/api/v1/references/10.1146/ANNUREV-BIOPHYS-062215-010822

CRISPR interference can prevent natural transformation and virulence acquisition during in vivo bacterial infection.

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9 October 2022

https://opencitations.net/index/coci/api/v1/references/10.1146/ANNUREV-BIOPHYS-062215-010822

High-throughput functional genomics using CRISPR-Cas9.

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9 October 2022

https://opencitations.net/index/coci/api/v1/references/10.1146/ANNUREV-BIOPHYS-062215-010822

Rational design of a split-Cas9 enzyme complex

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9 October 2022

https://opencitations.net/index/coci/api/v1/references/10.1146/ANNUREV-BIOPHYS-062215-010822

Beyond editing: repurposing CRISPR-Cas9 for precision genome regulation and interrogation

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9 October 2022

https://opencitations.net/index/coci/api/v1/references/10.1146/ANNUREV-BIOPHYS-062215-010822

Cut site selection by the two nuclease domains of the Cas9 RNA-guided endonuclease

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9 October 2022

https://opencitations.net/index/coci/api/v1/references/10.1146/ANNUREV-BIOPHYS-062215-010822

A putative RNA-interference-based immune system in prokaryotes: computational analysis of the predicted enzymatic machinery, functional analogies with eukaryotic RNAi, and hypothetical mechanisms of action

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9 October 2022

https://opencitations.net/index/coci/api/v1/references/10.1146/ANNUREV-BIOPHYS-062215-010822

Planting the seed: target recognition of short guide RNAs.

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9 October 2022

https://opencitations.net/index/coci/api/v1/references/10.1146/ANNUREV-BIOPHYS-062215-010822

An equivalent metal ion in one- and two-metal-ion catalysis

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9 October 2022

https://opencitations.net/index/coci/api/v1/references/10.1146/ANNUREV-BIOPHYS-062215-010822

RNA-guided complex from a bacterial immune system enhances target recognition through seed sequence interactions

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9 October 2022

https://opencitations.net/index/coci/api/v1/references/10.1146/ANNUREV-BIOPHYS-062215-010822

The discovery of CRISPR in archaea and bacteria

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9 October 2022

https://opencitations.net/index/coci/api/v1/references/10.1146/ANNUREV-BIOPHYS-062215-010822

Structures of Cas9 Endonucleases Reveal RNA-Mediated Conformational Activation

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9 October 2022

https://opencitations.net/index/coci/api/v1/references/10.1146/ANNUREV-BIOPHYS-062215-010822

Identifying and Visualizing Functional PAM Diversity across CRISPR-Cas Systems

1 reference

9 October 2022

https://opencitations.net/index/coci/api/v1/references/10.1146/ANNUREV-BIOPHYS-062215-010822

Processing-independent CRISPR RNAs limit natural transformation in Neisseria meningitidis

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9 October 2022

https://opencitations.net/index/coci/api/v1/references/10.1146/ANNUREV-BIOPHYS-062215-010822

Small CRISPR RNAs guide antiviral defense in prokaryotes

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9 October 2022

https://opencitations.net/index/coci/api/v1/references/10.1146/ANNUREV-BIOPHYS-062215-010822

Short motif sequences determine the targets of the prokaryotic CRISPR defence system

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9 October 2022

https://opencitations.net/index/coci/api/v1/references/10.1146/ANNUREV-BIOPHYS-062215-010822

Engineered CRISPR-Cas9 nucleases with altered PAM specificities

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9 October 2022

https://opencitations.net/index/coci/api/v1/references/10.1146/ANNUREV-BIOPHYS-062215-010822

Improving CRISPR-Cas nuclease specificity using truncated guide RNAs

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9 October 2022

https://opencitations.net/index/coci/api/v1/references/10.1146/ANNUREV-BIOPHYS-062215-010822

Genome-wide binding of the CRISPR endonuclease Cas9 in mammalian cells

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9 October 2022

https://opencitations.net/index/coci/api/v1/references/10.1146/ANNUREV-BIOPHYS-062215-010822

The structural biology of CRISPR-Cas systems

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9 October 2022

https://opencitations.net/index/coci/api/v1/references/10.1146/ANNUREV-BIOPHYS-062215-010822

Interference by clustered regularly interspaced short palindromic repeat (CRISPR) RNA is governed by a seed sequence

1 reference

9 October 2022

https://opencitations.net/index/coci/api/v1/references/10.1146/ANNUREV-BIOPHYS-062215-010822

Crystal Structure of Staphylococcus aureus Cas9

1 reference

9 October 2022

https://opencitations.net/index/coci/api/v1/references/10.1146/ANNUREV-BIOPHYS-062215-010822

Structural biology. Crystal structure of the CRISPR RNA-guided surveillance complex from Escherichia coli

1 reference

9 October 2022

https://opencitations.net/index/coci/api/v1/references/10.1146/ANNUREV-BIOPHYS-062215-010822

Cas9-crRNA ribonucleoprotein complex mediates specific DNA cleavage for adaptive immunity in bacteria

1 reference

9 October 2022

https://opencitations.net/index/coci/api/v1/references/10.1146/ANNUREV-BIOPHYS-062215-010822

Kinetics of the CRISPR-Cas9 effector complex assembly and the role of 3'-terminal segment of guide RNA.

1 reference

9 October 2022

https://opencitations.net/index/coci/api/v1/references/10.1146/ANNUREV-BIOPHYS-062215-010822

Dynamics of CRISPR-Cas9 genome interrogation in living cells

1 reference

9 October 2022

https://opencitations.net/index/coci/api/v1/references/10.1146/ANNUREV-BIOPHYS-062215-010822

Harnessing CRISPR-Cas9 immunity for genetic engineering

1 reference

9 October 2022

https://opencitations.net/index/coci/api/v1/references/10.1146/ANNUREV-BIOPHYS-062215-010822

A Programmable Dual-RNA-Guided DNA Endonuclease in Adaptive Bacterial Immunity

1 reference

9 October 2022

https://opencitations.net/index/coci/api/v1/references/10.1146/ANNUREV-BIOPHYS-062215-010822

CRISPR-Cas adaptation: insights into the mechanism of action

1 reference

9 October 2022

https://opencitations.net/index/coci/api/v1/references/10.1146/ANNUREV-BIOPHYS-062215-010822

Mechanism of eukaryotic homologous recombination

1 reference

9 October 2022

https://opencitations.net/index/coci/api/v1/references/10.1146/ANNUREV-BIOPHYS-062215-010822

Cas9 specifies functional viral targets during CRISPR-Cas adaptation.

1 reference

9 October 2022

https://opencitations.net/index/coci/api/v1/references/10.1146/ANNUREV-BIOPHYS-062215-010822

Broadening the targeting range of Staphylococcus aureus CRISPR-Cas9 by modifying PAM recognition

1 reference

9 October 2022

https://opencitations.net/index/coci/api/v1/references/10.1146/ANNUREV-BIOPHYS-062215-010822

Identifiers

DOI

10.1146/ANNUREV-BIOPHYS-062215-010822

1 reference

stated in

Europe PubMed Central

10 September 2017

release_yco6nenkd5d4bf2pnho35splnq

1 reference

stated in

Fatcat

reference URL

https://api.fatcat.wiki/v0/release/yco6nenkd5d4bf2pnho35splnq

retrieved

24 November 2022

based on heuristic

mapped directly with Wikidata item

PubMed ID

28375731

1 reference

stated in

Europe PubMed Central

PubMed ID

28375731

retrieved

10 September 2017

(Q39219057)

CRISPR-Cas9 Structures and Mechanisms

Statements

Identifiers

Sitelinks

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