(Q36575382)

15 August 2017

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title

On the art of identifying effective and specific siRNAs (English)

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15 August 2017

2

Thomas Tuschl

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15 August 2017

Yi Pei

1

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publication date

1 September 2006

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15 August 2017

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15 August 2017

volume

3

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15 August 2017

issue

9

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15 August 2017

page(s)

670-676

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Mechanisms of gene silencing by double-stranded RNA

15 August 2017

cites work

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7 January 2021

siRNAs: applications in functional genomics and potential as therapeutics

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7 January 2021

High-throughput RNAi screening in cultured cells: a user's guide.

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7 January 2021

The silent treatment: siRNAs as small molecule drugs

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7 January 2021

Analysis of gene function in somatic mammalian cells using small interfering RNAs

1 reference

7 January 2021

Rational siRNA design for RNA interference

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7 January 2021

Positional effects of short interfering RNAs targeting the human coagulation trigger Tissue Factor

1 reference

7 January 2021

Sequence, chemical, and structural variation of small interfering RNAs and short hairpin RNAs and the effect on mammalian gene silencing

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7 January 2021

3' UTR seed matches, but not overall identity, are associated with RNAi off-targets

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7 January 2021

Noise amidst the silence: off-target effects of siRNAs?

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7 January 2021

siRNA-mediated off-target gene silencing triggered by a 7 nt complementation

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7 January 2021

Widespread siRNA "off-target" transcript silencing mediated by seed region sequence complementarity

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7 January 2021

Off-target effects by siRNA can induce toxic phenotype

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7 January 2021

Design of a genome-wide siRNA library using an artificial neural network

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7 January 2021

A comparison of siRNA efficacy predictors.

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7 January 2021

High-throughput screening of effective siRNAs from RNAi libraries delivered via bacterial invasion

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7 January 2021

Genome-wide application of RNAi to the discovery of potential drug targets

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7 January 2021

Screening of siRNA target sequences by using fragmentized DNA.

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7 January 2021

Assembly and function of RNA silencing complexes

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7 January 2021

Perspective: machines for RNAi

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7 January 2021

Post-transcriptional gene silencing by siRNAs and miRNAs

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7 January 2021

Control of translation and mRNA degradation by miRNAs and siRNAs

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7 January 2021

A library of siRNA duplexes targeting the phosphoinositide 3-kinase pathway: determinants of gene silencing for use in cell-based screens

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7 January 2021

Small interfering RNA-mediated gene silencing in T lymphocytes

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7 January 2021

siRecords: an extensive database of mammalian siRNAs with efficacy ratings.

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7 January 2021

siRNAdb: a database of siRNA sequences

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7 January 2021

HuSiDa--the human siRNA database: an open-access database for published functional siRNA sequences and technical details of efficient transfer into recipient cells

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7 January 2021

Computational models with thermodynamic and composition features improve siRNA design

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7 January 2021

siRNA Selection Server: an automated siRNA oligonucleotide prediction server

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7 January 2021

Improved and automated prediction of effective siRNA.

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7 January 2021

Guidelines for the selection of highly effective siRNA sequences for mammalian and chick RNA interference

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7 January 2021

Sequence characteristics of functional siRNAs

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7 January 2021

An algorithm for selection of functional siRNA sequences.

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7 January 2021

Sfold web server for statistical folding and rational design of nucleic acids

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7 January 2021

The gene-silencing efficiency of siRNA is strongly dependent on the local structure of mRNA at the targeted region

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7 January 2021

Filtering of ineffective siRNAs and improved siRNA design tool.

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7 January 2021

High-throughput selection of effective RNAi probes for gene silencing.

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7 January 2021

Comparison of test systems for RNAinterference

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7 January 2021

Enhancing and confirming the specificity of RNAi experiments

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7 January 2021

Functional siRNAs and miRNAs Exhibit Strand Bias

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7 January 2021

Asymmetry in the assembly of the RNAi enzyme complex

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7 January 2021

Small RNA asymmetry in RNAi: function in RISC assembly and gene regulation.

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7 January 2021

Design of siRNAs producing unstructured guide-RNAs results in improved RNA interference efficiency.

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7 January 2021

Criteria for effective design, construction, and gene knockdown by shRNA vectors

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7 January 2021

Passenger-strand cleavage facilitates assembly of siRNA into Ago2-containing RNAi enzyme complexes

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7 January 2021

RNA interference and chemically modified small interfering RNAs

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7 January 2021

Induction of stable RNA interference in mammalian cells

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7 January 2021

siRNA target site secondary structure predictions using local stable substructures

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7 January 2021

Local RNA target structure influences siRNA efficacy: systematic analysis of intentionally designed binding regions

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7 January 2021

Local RNA target structure influences siRNA efficacy: a systematic global analysis

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7 January 2021

Improving the efficiency of RNA interference in mammals

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7 January 2021

Sequence-dependent stimulation of the mammalian innate immune response by synthetic siRNA

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7 January 2021

Sequence-specific potent induction of IFN-alpha by short interfering RNA in plasmacytoid dendritic cells through TLR7

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7 January 2021

Activation of the mammalian immune system by siRNAs.

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7 January 2021

Design of noninflammatory synthetic siRNA mediating potent gene silencing in vivo

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7 January 2021

Expressing short hairpin RNAs in vivo

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7 January 2021

Duplexes of 21-nucleotide RNAs mediate RNA interference in cultured mammalian cells

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7 January 2021

RNA interference is mediated by 21- and 22-nucleotide RNAs

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7 January 2021

Specific inhibition of gene expression by small double-stranded RNAs in invertebrate and vertebrate systems

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7 January 2021

Short hairpin RNAs (shRNAs) induce sequence-specific silencing in mammalian cells

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7 January 2021

Synthetic dsRNA Dicer substrates enhance RNAi potency and efficacy.

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7 January 2021

Approaches for chemically synthesized siRNA and vector-mediated RNAi

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7 January 2021

Functional polarity is introduced by Dicer processing of short substrate RNAs

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7 January 2021

Induction of the interferon response by siRNA is cell type- and duplex length-dependent

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7 January 2021

A structural basis for discriminating between self and nonself double-stranded RNAs in mammalian cells.

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7 January 2021

The contributions of dsRNA structure to Dicer specificity and efficiency

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7 January 2021

Therapeutic silencing of an endogenous gene by systemic administration of modified siRNAs

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7 January 2021

Expression profiling reveals off-target gene regulation by RNAi

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7 January 2021

MicroRNAs: genomics, biogenesis, mechanism, and function

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7 January 2021

Kinetic analysis of the RNAi enzyme complex

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7 January 2021

RISC is a 5' phosphomonoester-producing RNA endonuclease

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7 January 2021

Tolerated wobble mutations in siRNAs decrease specificity, but can enhance activity in vivo

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7 January 2021

Functional anatomy of siRNAs for mediating efficient RNAi in Drosophila melanogaster embryo lysate

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7 January 2021

A systematic analysis of the silencing effects of an active siRNA at all single-nucleotide mismatched target sites

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7 January 2021

Determinants of specific RNA interference-mediated silencing of human beta-globin alleles differing by a single nucleotide polymorphism

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7 January 2021

Mechanistic insights aid computational short interfering RNA design.

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7 January 2021

Highly specific and accurate selection of siRNAs for high-throughput functional assays

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7 January 2021

siDirect: highly effective, target-specific siRNA design software for mammalian RNA interference

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7 January 2021

Position-specific chemical modification of siRNAs reduces "off-target" transcript silencing

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7 January 2021

Allele-specific RNA interference for neurological disease

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7 January 2021

New RNAi strategy for selective suppression of a mutant allele in polyglutamine disease

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7 January 2021

A genetic strategy to treat sickle cell anemia by coregulating globin transgene expression and RNA interference

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7 January 2021

Single-stranded antisense siRNAs guide target RNA cleavage in RNAi

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7 January 2021

Production of endoribonuclease-prepared short interfering RNAs for gene silencing in mammalian cells

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7 January 2021

Enzymatically prepared RNAi libraries

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7 January 2021

shRNA libraries and their use in cancer genetics

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7 January 2021

Lessons from Nature: microRNA-based shRNA libraries

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7 January 2021

Genome-scale loss-of-function screening with a lentiviral RNAi library

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7 January 2021

MicroRNA function: multiple mechanisms for a tiny RNA?

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7 January 2021

MicroRNA-directed cleavage of HOXB8 mRNA

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7 January 2021

Argonaute2 cleaves the anti-guide strand of siRNA during RISC activation

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7 January 2021

Cleavage of the siRNA passenger strand during RISC assembly in human cells

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7 January 2021

Identifiers

DOI

10.1038/NMETH911

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15 August 2017

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