(Q38682882)

5 September 2017

Creating and evaluating accurate CRISPR-Cas9 scalpels for genomic surgery (English)

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5 September 2017

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Mehmet Fatih Bolukbasi

series ordinal

1

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5 September 2017

Ankit Gupta

series ordinal

2

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5 September 2017

Scot A Wolfe

series ordinal

3

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5 September 2017

1 December 2015

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5 September 2017

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5 September 2017

13

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5 September 2017

1

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5 September 2017

41-50

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https://scigraph.springernature.com/pub.10.1038/nmeth.3684

5 September 2017

0 references

cites work

Classification and evolution of type II CRISPR-Cas systems

1 reference

https://api.crossref.org/works/10.1038%2FNMETH.3684

An updated evolutionary classification of CRISPR-Cas systems

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNMETH.3684

The Bacterial Origins of the CRISPR Genome-Editing Revolution

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNMETH.3684

A Programmable Dual-RNA-Guided DNA Endonuclease in Adaptive Bacterial Immunity

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNMETH.3684

Genome editing with engineered zinc finger nucleases

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNMETH.3684

TALENs: a widely applicable technology for targeted genome editing

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNMETH.3684

Multiplex genome engineering using CRISPR/Cas systems

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNMETH.3684

RNA-guided human genome engineering via Cas9

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNMETH.3684

Targeted genome engineering in human cells with the Cas9 RNA-guided endonuclease

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNMETH.3684

RNA-programmed genome editing in human cells

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNMETH.3684

One-step generation of mice carrying mutations in multiple genes by CRISPR/Cas-mediated genome engineering

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNMETH.3684

Efficient genome editing in zebrafish using a CRISPR-Cas system

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNMETH.3684

Dynamic imaging of genomic loci in living human cells by an optimized CRISPR/Cas system

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNMETH.3684

21 January 2018

CRISPR, the disruptor

1 reference

https://api.crossref.org/works/10.1038%2FNMETH.3684

Genome editing. The new frontier of genome engineering with CRISPR-Cas9

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNMETH.3684

Therapeutic genome editing: prospects and challenges

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNMETH.3684

Efficient multiplex biallelic zebrafish genome editing using a CRISPR nuclease system

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNMETH.3684

In vivo interrogation of gene function in the mammalian brain using CRISPR-Cas9

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNMETH.3684

Multiplex CRISPR/Cas9-based genome engineering from a single lentiviral vector

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNMETH.3684

Efficient ablation of genes in human hematopoietic stem and effector cells using CRISPR/Cas9

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNMETH.3684

Dimeric CRISPR RNA-guided FokI nucleases for highly specific genome editing

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNMETH.3684

Chemically modified guide RNAs enhance CRISPR-Cas genome editing in human primary cells

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNMETH.3684

One-step generation of mice carrying reporter and conditional alleles by CRISPR/Cas-mediated genome engineering

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNMETH.3684

Characterization of genomic deletion efficiency mediated by clustered regularly interspaced palindromic repeats (CRISPR)/Cas9 nuclease system in mammalian cells.

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNMETH.3684

Disruptions of topological chromatin domains cause pathogenic rewiring of gene-enhancer interactions

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNMETH.3684

Engineering human tumour-associated chromosomal translocations with the RNA-guided CRISPR-Cas9 system

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNMETH.3684

Chromosomal translocations in human cells are generated by canonical nonhomologous end-joining.

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNMETH.3684

Analysis of off-target effects of CRISPR/Cas-derived RNA-guided endonucleases and nickases.

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNMETH.3684

Genetic screens in human cells using the CRISPR-Cas9 system

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNMETH.3684

Genome-scale CRISPR-Cas9 knockout screening in human cells

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNMETH.3684

Genome-wide recessive genetic screening in mammalian cells with a lentiviral CRISPR-guide RNA library

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNMETH.3684

High-throughput screening of a CRISPR/Cas9 library for functional genomics in human cells

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNMETH.3684

CRISPR-Cas9 knockin mice for genome editing and cancer modeling

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNMETH.3684

A Genome-wide CRISPR Screen in Primary Immune Cells to Dissect Regulatory Networks

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNMETH.3684

Multi-kilobase homozygous targeted gene replacement in human induced pluripotent stem cells

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNMETH.3684

Optimization of scarless human stem cell genome editing

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNMETH.3684

Genome editing with Cas9 in adult mice corrects a disease mutation and phenotype.

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNMETH.3684

Generation of knock-in primary human T cells using Cas9 ribonucleoproteins.

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNMETH.3684

Adenoviral vector DNA for accurate genome editing with engineered nucleases.

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNMETH.3684

Efficient CRISPR-rAAV engineering of endogenous genes to study protein function by allele-specific RNAi

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNMETH.3684

Quantifying genome-editing outcomes at endogenous loci with SMRT sequencing

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNMETH.3684

Increasing the efficiency of homology-directed repair for CRISPR-Cas9-induced precise gene editing in mammalian cells.

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNMETH.3684

Increasing the efficiency of precise genome editing with CRISPR-Cas9 by inhibition of nonhomologous end joining

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNMETH.3684

Rapid modelling of cooperating genetic events in cancer through somatic genome editing

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNMETH.3684

CRISPR-mediated direct mutation of cancer genes in the mouse liver

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNMETH.3684

A CRISPR/Cas9 vector system for tissue-specific gene disruption in zebrafish

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNMETH.3684

Adenovirus-Mediated Somatic Genome Editing of Pten by CRISPR/Cas9 in Mouse Liver in Spite of Cas9-Specific Immune Responses.

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNMETH.3684

Somatic CRISPR/Cas9-mediated tumour suppressor disruption enables versatile brain tumour modelling

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNMETH.3684

Applications of the CRISPR-Cas9 system in cancer biology

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNMETH.3684

Modeling the mitochondrial cardiomyopathy of Barth syndrome with induced pluripotent stem cell and heart-on-chip technologies

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNMETH.3684

Genome-wide CRISPR screen in a mouse model of tumor growth and metastasis

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNMETH.3684

In vivo engineering of oncogenic chromosomal rearrangements with the CRISPR/Cas9 system.

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNMETH.3684

Simple and rapid in vivo generation of chromosomal rearrangements using CRISPR/Cas9 technology

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNMETH.3684

Precise correction of the dystrophin gene in duchenne muscular dystrophy patient induced pluripotent stem cells by TALEN and CRISPR-Cas9.

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNMETH.3684

Functional Correction of Large Factor VIII Gene Chromosomal Inversions in Hemophilia A Patient-Derived iPSCs Using CRISPR-Cas9.

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNMETH.3684

Delivery and Specificity of CRISPR-Cas9 Genome Editing Technologies for Human Gene Therapy.

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNMETH.3684

Effect of genome size on AAV vector packaging

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNMETH.3684

Efficient genome engineering in human pluripotent stem cells using Cas9 from Neisseria meningitidis

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNMETH.3684

Processing-independent CRISPR RNAs limit natural transformation in Neisseria meningitidis

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNMETH.3684

In vivo genome editing using Staphylococcus aureus Cas9

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNMETH.3684

Insertional oncogenesis in 4 patients after retrovirus-mediated gene therapy of SCID-X1

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNMETH.3684

Genomic instability and myelodysplasia with monosomy 7 consequent to EVI1 activation after gene therapy for chronic granulomatous disease

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNMETH.3684

DNA interrogation by the CRISPR RNA-guided endonuclease Cas9.

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNMETH.3684

Direct observation of R-loop formation by single RNA-guided Cas9 and Cascade effector complexes.

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNMETH.3684

Genome-wide binding of the CRISPR endonuclease Cas9 in mammalian cells

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNMETH.3684

Structural basis of PAM-dependent target DNA recognition by the Cas9 endonuclease

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNMETH.3684

Structures of Cas9 Endonucleases Reveal RNA-Mediated Conformational Activation

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNMETH.3684

Crystal Structure of Cas9 in Complex with Guide RNA and Target DNA

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNMETH.3684

STRUCTURAL BIOLOGY. A Cas9-guide RNA complex preorganized for target DNA recognition

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNMETH.3684

DNA targeting specificity of RNA-guided Cas9 nucleases

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNMETH.3684

High-frequency off-target mutagenesis induced by CRISPR-Cas nucleases in human cells

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNMETH.3684

Unraveling CRISPR-Cas9 genome engineering parameters via a library-on-library approach

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNMETH.3684

Genome-wide analysis reveals characteristics of off-target sites bound by the Cas9 endonuclease.

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNMETH.3684

Comparison of non-canonical PAMs for CRISPR/Cas9-mediated DNA cleavage in human cells

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNMETH.3684

CAS9 transcriptional activators for target specificity screening and paired nickases for cooperative genome engineering

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNMETH.3684

Cas9-crRNA ribonucleoprotein complex mediates specific DNA cleavage for adaptive immunity in bacteria

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNMETH.3684

Efficient mutagenesis by Cas9 protein-mediated oligonucleotide insertion and large-scale assessment of single-guide RNAs

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNMETH.3684

Rational design of highly active sgRNAs for CRISPR-Cas9-mediated gene inactivation.

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNMETH.3684

Dramatic enhancement of genome editing by CRISPR/Cas9 through improved guide RNA design

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNMETH.3684

Sequence determinants of improved CRISPR sgRNA design

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNMETH.3684

CHOPCHOP: a CRISPR/Cas9 and TALEN web tool for genome editing

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNMETH.3684

CRISPRscan: designing highly efficient sgRNAs for CRISPR-Cas9 targeting in vivo

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNMETH.3684

High-throughput profiling of off-target DNA cleavage reveals RNA-programmed Cas9 nuclease specificity

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNMETH.3684

CRISPR/Cas9 systems have off-target activity with insertions or deletions between target DNA and guide RNA sequences

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNMETH.3684

Whole-genome sequencing analysis reveals high specificity of CRISPR/Cas9 and TALEN-based genome editing in human iPSCs

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNMETH.3684

Low incidence of off-target mutations in individual CRISPR-Cas9 and TALEN targeted human stem cell clones detected by whole-genome sequencing.

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNMETH.3684

Digenome-seq: genome-wide profiling of CRISPR-Cas9 off-target effects in human cells

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNMETH.3684

Off-target mutations are rare in Cas9-modified mice.

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNMETH.3684

Efficient genome modification by CRISPR-Cas9 nickase with minimal off-target effects.

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNMETH.3684

High-throughput gene targeting and phenotyping in zebrafish using CRISPR/Cas9.

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNMETH.3684

CRISPRseek: a bioconductor package to identify target-specific guide RNAs for CRISPR-Cas9 genome-editing systems

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNMETH.3684

Cas-OFFinder: a fast and versatile algorithm that searches for potential off-target sites of Cas9 RNA-guided endonucleases

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNMETH.3684

COSMID: A Web-based Tool for Identifying and Validating CRISPR/Cas Off-target Sites

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNMETH.3684

Cas9-chromatin binding information enables more accurate CRISPR off-target prediction.

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNMETH.3684

E-CRISP: fast CRISPR target site identification.

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNMETH.3684

ZiFiT (Zinc Finger Targeter): an updated zinc finger engineering tool

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNMETH.3684

CasOT: a genome-wide Cas9/gRNA off-target searching tool

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNMETH.3684

GUIDE-seq enables genome-wide profiling of off-target cleavage by CRISPR-Cas nucleases

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNMETH.3684

Genome-wide detection of DNA double-stranded breaks induced by engineered nucleases.

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNMETH.3684

Unbiased detection of off-target cleavage by CRISPR-Cas9 and TALENs using integrase-defective lentiviral vectors.

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNMETH.3684

Nucleotide-resolution DNA double-strand break mapping by next-generation sequencing

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNMETH.3684

An unbiased genome-wide analysis of zinc-finger nuclease specificity

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNMETH.3684

Improving CRISPR-Cas nuclease specificity using truncated guide RNAs

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNMETH.3684

Highly efficient RNA-guided genome editing in human cells via delivery of purified Cas9 ribonucleoproteins

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNMETH.3684

Gene disruption by cell-penetrating peptide-mediated delivery of Cas9 protein and guide RNA.

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNMETH.3684

Cationic lipid-mediated delivery of proteins enables efficient protein-based genome editing in vitro and in vivo

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNMETH.3684

Double nicking by RNA-guided CRISPR Cas9 for enhanced genome editing specificity

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNMETH.3684

Dimeric CRISPR RNA-Guided FokI-dCas9 Nucleases Directed by Truncated gRNAs for Highly Specific Genome Editing

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNMETH.3684

Fusion of catalytically inactive Cas9 to FokI nuclease improves the specificity of genome modification

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNMETH.3684

Engineered CRISPR-Cas9 nucleases with altered PAM specificities

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNMETH.3684

Rational design of a split-Cas9 enzyme complex

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNMETH.3684

A split-Cas9 architecture for inducible genome editing and transcription modulation

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNMETH.3684

Photoactivatable CRISPR-Cas9 for optogenetic genome editing

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNMETH.3684

Development of an intein-mediated split-Cas9 system for gene therapy

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNMETH.3684

Small molecule-triggered Cas9 protein with improved genome-editing specificity

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNMETH.3684

Phylogeny of Cas9 determines functional exchangeability of dual-RNA and Cas9 among orthologous type II CRISPR-Cas systems

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNMETH.3684

Orthogonal Cas9 proteins for RNA-guided gene regulation and editing

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNMETH.3684

Cpf1 is a single RNA-guided endonuclease of a class 2 CRISPR-Cas system

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNMETH.3684

Quantifying on- and off-target genome editing

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNMETH.3684

Discovery of cancer drug targets by CRISPR-Cas9 screening of protein domains

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNMETH.3684

Reverse genetic screening reveals poor correlation between morpholino-induced and mutant phenotypes in zebrafish.

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNMETH.3684

Chromosomal deletions and inversions mediated by TALENs and CRISPR/Cas in zebrafish.

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNMETH.3684

Efficient in vivo deletion of a large imprinted lncRNA by CRISPR/Cas9.

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNMETH.3684

A gene regulatory network controls the binary fate decision of rod and bipolar cells in the vertebrate retina

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNMETH.3684

Global microRNA depletion suppresses tumor angiogenesis.

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNMETH.3684

Convergence of developmental and oncogenic signaling pathways at transcriptional super-enhancers

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNMETH.3684

In vivo genome editing using a high-efficiency TALEN system

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNMETH.3684

Heritable and precise zebrafish genome editing using a CRISPR-Cas system

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNMETH.3684

In vivo genome editing of the albumin locus as a platform for protein replacement therapy

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNMETH.3684

Polyclonal long-term repopulating stem cell clones in a primate model

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNMETH.3684

Genome-wide translocation sequencing reveals mechanisms of chromosome breaks and rearrangements in B cells

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNMETH.3684

Structure and specificity of the RNA-guided endonuclease Cas9 during DNA interrogation, target binding and cleavage

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNMETH.3684

Targeted genomic rearrangements using CRISPR/Cas technology

21 January 2018

1 reference

12 December 2020

DNA-binding-domain fusions enhance the targeting range and precision of Cas9.

1 reference

12 December 2020

Enhanced homology-directed human genome engineering by controlled timing of CRISPR/Cas9 delivery

1 reference

12 December 2020

Efficient intracellular delivery of native proteins

1 reference

12 December 2020

What's changed with genome editing?

1 reference

12 December 2020

Functional disruption of the dystrophin gene in rhesus monkey using CRISPR/Cas9.

1 reference

12 December 2020

Identifiers

DOI

10.1038/NMETH.3684

1 reference

Dimensions Publication ID

5 September 2017

0 references

1 reference