(Q26866010)

title

Large-scale production and protein engineering of G protein-coupled receptors for structural studies (English)

1 reference

G protein-coupled receptor

main subject

0 references

author

Dalibor Milić

series ordinal

1

1 reference

https://www.ebi.ac.uk/europepmc/webservices/rest/search?query=EXT_ID:25873898%20AND%20SRC:MED&resulttype=core&format=json

9 June 2020

Dmitry B Veprintsev

series ordinal

2

1 reference

https://www.ebi.ac.uk/europepmc/webservices/rest/search?query=EXT_ID:25873898%20AND%20SRC:MED&resulttype=core&format=json

9 June 2020

author name string

Dmitry B. Veprintsev

series ordinal

2

1 reference

language of work or name

1 reference

2015

1 reference

Frontiers in Pharmacology

published in

2 references

https://www.ebi.ac.uk/europepmc/webservices/rest/search?query=EXT_ID:25873898%20AND%20SRC:MED&resulttype=core&format=json

9 June 2020

volume

6

2 references

https://www.ebi.ac.uk/europepmc/webservices/rest/search?query=EXT_ID:25873898%20AND%20SRC:MED&resulttype=core&format=json

9 June 2020

page(s)

66

2 references

https://www.ebi.ac.uk/europepmc/webservices/rest/search?query=EXT_ID:25873898%20AND%20SRC:MED&resulttype=core&format=json

High-resolution structure of the human GPR40 receptor bound to allosteric agonist TAK-875

9 June 2020

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Structure of a nanobody-stabilized active state of the β(2) adrenoceptor

21 March 2017

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Structures of the CXCR4 chemokine GPCR with small-molecule and cyclic peptide antagonists

21 March 2017

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The 2.6 angstrom crystal structure of a human A2A adenosine receptor bound to an antagonist

21 March 2017

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High-resolution crystal structure of an engineered human beta2-adrenergic G protein-coupled receptor

21 March 2017

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Crystal structure of a photoactivated deprotonated intermediate of rhodopsin

21 March 2017

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21 March 2017

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Crystal structure of the human OX2 orexin receptor bound to the insomnia drug suvorexant

21 March 2017

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High-resolution structure of a membrane protein transferred from amphipol to a lipidic mesophase

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Structure of class C GPCR metabotropic glutamate receptor 5 transmembrane domain

21 March 2017

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Structural basis for Smoothened receptor modulation and chemoresistance to anticancer drugs

21 March 2017

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Covalent agonists for studying G protein-coupled receptor activation

21 March 2017

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Agonist-bound structure of the human P2Y12 receptor

21 March 2017

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Structure of the human P2Y12 receptor in complex with an antithrombotic drug

21 March 2017

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The 2.1 Å Resolution Structure of Cyanopindolol-Bound β1-Adrenoceptor Identifies an Intramembrane Na+ Ion that Stabilises the Ligand-Free Receptor

21 March 2017

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Lipidic cubic phase injector facilitates membrane protein serial femtosecond crystallography

21 March 2017

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Structure of signaling-competent neurotensin receptor 1 obtained by directed evolution in Escherichia coli

21 March 2017

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21 March 2017

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Serial Femtosecond Crystallography of G Protein-Coupled Receptors

21 March 2017

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Structural insights into the role of the Smoothened cysteine-rich domain in Hedgehog signalling

21 March 2017

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21 March 2017

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21 March 2017

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Structure of the CCR5 Chemokine Receptor-HIV Entry Inhibitor Maraviroc Complex

21 March 2017

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Structure of the human smoothened receptor bound to an antitumour agent

21 March 2017

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Insights into congenital stationary night blindness based on the structure of G90D rhodopsin

21 March 2017

1 reference

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Structural Features for Functional Selectivity at Serotonin Receptors

21 March 2017

1 reference

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Structural Basis for Molecular Recognition at Serotonin Receptors

21 March 2017

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Biophysical Fragment Screening of the β 1 -Adrenergic Receptor: Identification of High Affinity Arylpiperazine Leads Using Structure-Based Drug Design

21 March 2017

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Crystal structure of oligomeric β1-adrenergic G protein–coupled receptors in ligand-free basal state

21 March 2017

1 reference

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Optimized Phospholipid Bilayer Nanodiscs Facilitate High-Resolution Structure Determination of Membrane Proteins

21 March 2017

1 reference

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High-resolution crystal structure of human protease-activated receptor 1

21 March 2017

1 reference

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Structure of the chemokine receptor CXCR1 in phospholipid bilayers

21 March 2017

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N-Terminal T4 Lysozyme Fusion Facilitates Crystallization of a G Protein Coupled Receptor

21 March 2017

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Structure of the agonist-bound neurotensin receptor

21 March 2017

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Structural Basis for Allosteric Regulation of GPCRs by Sodium Ions

21 March 2017

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Structure of the δ-opioid receptor bound to naltrindole

21 March 2017

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Structure of the nociceptin/orphanin FQ receptor in complex with a peptide mimetic

21 March 2017

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Crystal Structures of a Stabilized β1-Adrenoceptor Bound to the Biased Agonists Bucindolol and Carvedilol

21 March 2017

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Crystal structure of the µ-opioid receptor bound to a morphinan antagonist

21 March 2017

1 reference

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Structure and dynamics of the M3 muscarinic acetylcholine receptor

21 March 2017

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G-protein-coupled receptor inactivation by an allosteric inverse-agonist antibody

21 March 2017

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Discovery of 1,2,4-Triazine Derivatives as Adenosine A 2A Antagonists using Structure Based Drug Design

21 March 2017

1 reference

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Stabilized G protein binding site in the structure of constitutively active metarhodopsin-II

21 March 2017

1 reference

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Solution NMR Structure of Proteorhodopsin

21 March 2017

1 reference

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Crystallographic analysis of the primary photochemical reaction of squid rhodopsin

21 March 2017

1 reference

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Structure of the Adenosine A2A Receptor in Complex with ZM241385 and the Xanthines XAC and Caffeine

21 March 2017

1 reference

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Nanobody stabilization of G protein-coupled receptor conformational states.

21 March 2017

1 reference

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Crystal structure of the eukaryotic light-driven proton-pumping rhodopsin, Acetabularia rhodopsin II, from marine alga

21 March 2017

1 reference

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Two distinct conformations of helix 6 observed in antagonist-bound structures of a 1-adrenergic receptor

21 March 2017

1 reference

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Crystal structure of metarhodopsin II

21 March 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4379943

The structural basis of agonist-induced activation in constitutively active rhodopsin

21 March 2017

1 reference

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Structure of the Human Dopamine D3 Receptor in Complex with a D2/D3 Selective Antagonist

21 March 2017

1 reference

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Binding of More Than One Retinoid to Visual Opsins

21 March 2017

1 reference

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Conserved Binding Mode of Human β 2 Adrenergic Receptor Inverse Agonists and Antagonist Revealed by X-ray Crystallography

21 March 2017

1 reference

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Structure determination of the seven-helix transmembrane receptor sensory rhodopsin II by solution NMR spectroscopy

21 March 2017

1 reference

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Ligand-specific regulation of the extracellular surface of a G-protein-coupled receptor

21 March 2017

1 reference

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Crystal structure of opsin in its G-protein-interacting conformation

21 March 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4379943

Structure of a beta1-adrenergic G-protein-coupled receptor

21 March 2017

1 reference

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Crystal structure of the ligand-free G-protein-coupled receptor opsin

21 March 2017

1 reference

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A Specific Cholesterol Binding Site Is Established by the 2.8 Å Structure of the Human β2-Adrenergic Receptor

21 March 2017

1 reference

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Crystal structure of squid rhodopsin

21 March 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4379943

Crystal Structure of Squid Rhodopsin with Intracellularly Extended Cytoplasmic Region

21 March 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4379943

Crystal structure of the human beta2 adrenergic G-protein-coupled receptor

21 March 2017

2 references

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4379943

21 March 2017

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4379943

Crystal Structure of a Thermally Stable Rhodopsin Mutant

7 April 2017

1 reference

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Photoisomerization Mechanism of Rhodopsin and 9-cis-Rhodopsin Revealed by X-ray Crystallography

21 March 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4379943

Functional role of internal water molecules in rhodopsin revealed by X-ray crystallography.

21 March 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4379943

Structural views of the ligand-binding cores of a metabotropic glutamate receptor complexed with an antagonist and both glutamate and Gd3+.

21 March 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4379943

Insights into Wnt binding and signalling from the structures of two Frizzled cysteine-rich domains

21 March 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4379943

Advances in Determination of a High-Resolution Three-Dimensional Structure of Rhodopsin, a Model of G-Protein-Coupled Receptors (GPCRs)

21 March 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4379943

Structural basis of glutamate recognition by a dimeric metabotropic glutamate receptor

21 March 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4379943

Crystal structure of rhodopsin: A G protein-coupled receptor

21 March 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4379943

Directed evolution of a G protein-coupled receptor for expression, stability, and binding selectivity

21 March 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4379943

GPCR engineering yields high-resolution structural insights into beta2-adrenergic receptor function

21 March 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4379943

A robust and rapid method of producing soluble, stable, and functional G-protein coupled receptors

21 March 2017

1 reference

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Structural biology. Crystal structure of the chemokine receptor CXCR4 in complex with a viral chemokine

21 March 2017

1 reference

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Designer lipid-like peptides: a class of detergents for studying functional olfactory receptors using commercial cell-free systems

21 March 2017

1 reference

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Crystallizing membrane proteins using lipidic mesophases

21 March 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4379943

Structure and function in rhodopsin: high-level expression of rhodopsin with restricted and homogeneous N-glycosylation by a tetracycline-inducible N-acetylglucosaminyltransferase I-negative HEK293S stable mammalian cell line

7 April 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4379943

Structural biology. Structural basis for chemokine recognition and activation of a viral G protein-coupled receptor

7 April 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4379943

A comprehensive review of the lipid cubic phase or in meso method for crystallizing membrane and soluble proteins and complexes.

28 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4379943

Isotopic labeling of mammalian G protein-coupled receptors heterologously expressed in Caenorhabditis elegans

28 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4379943

Quality control in eukaryotic membrane protein overproduction

28 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4379943

Modified T4 Lysozyme Fusion Proteins Facilitate G Protein-Coupled Receptor Crystallogenesis

28 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4379943

Rapid Computational Prediction of Thermostabilizing Mutations for G Protein-Coupled Receptors.

28 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4379943

GPCR crystallization using lipidic cubic phase technique

28 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4379943

Generating thermostabilized agonist-bound GPR40/FFAR1 using virus-like particles and a label-free binding assay

28 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4379943

Femtosecond crystallography of membrane proteins in the lipidic cubic phase

28 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4379943

Amphipols in G protein-coupled receptor pharmacology: what are they good for?

28 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4379943

The use of amphipols for solution NMR studies of membrane proteins: advantages and constraints as compared to other solubilizing media

28 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4379943

The Concise Guide to PHARMACOLOGY 2013/14: G protein-coupled receptors

28 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4379943

Structure-based drug design for G protein-coupled receptors.

28 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4379943

Global fold of human cannabinoid type 2 receptor probed by solid-state 13C-, 15N-MAS NMR and molecular dynamics simulations

28 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4379943

Structural and functional insights into the juxtamembranous amino-terminal tail and extracellular loop regions of class B GPCRs.

28 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4379943

A computationally designed water-soluble variant of a G-protein-coupled receptor: the human mu opioid receptor

28 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4379943

Production of GPCR and GPCR complexes for structure determination

28 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4379943

Surface plasmon resonance spectroscopy for characterisation of membrane protein-ligand interactions and its potential for drug discovery

28 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4379943

Cell-free expression--making a mark.

28 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4379943

Molecular signatures of G-protein-coupled receptors

28 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4379943

The dynamic process of β(2)-adrenergic receptor activation

28 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4379943

How ligands and signalling proteins affect G-protein-coupled receptors' conformational landscape

28 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4379943

Direct molecular evolution of detergent-stable G protein-coupled receptors using polymer encapsulated cells

28 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4379943

Efficacy of the β₂-adrenergic receptor is determined by conformational equilibrium in the transmembrane region

28 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4379943

A fluorescence-detection size-exclusion chromatography-based thermostability assay for membrane protein precrystallization screening

28 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4379943

Ligand-receptor interaction platforms and their applications for drug discovery.

28 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4379943

A new era of GPCR structural and chemical biology

28 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4379943

A crystal clear solution for determining G-protein-coupled receptor structures

28 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4379943

Maximizing detergent stability and functional expression of a GPCR by exhaustive recombination and evolution

28 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4379943

Fusion partner toolchest for the stabilization and crystallization of G protein-coupled receptors

28 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4379943

Critical features for biosynthesis, stability, and functionality of a G protein-coupled receptor uncovered by all-versus-all mutations

28 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4379943

Mistic and TarCF as fusion protein partners for functional expression of the cannabinoid receptor 2 in Escherichia coli

28 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4379943

Overexpressing human membrane proteins in stably transfected and clonal human embryonic kidney 293S cells

28 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4379943

Heterologous expression of functional G-protein-coupled receptors in Caenorhabditis elegans

28 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4379943

Optimization of purification and refolding of the human chemokine receptor CXCR1 improves the stability of proteoliposomes for structure determination

28 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4379943

Crystallizing membrane proteins using lipidic bicelles

28 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4379943

Ligand discovery from a dopamine D3 receptor homology model and crystal structure

28 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4379943

G protein-coupled receptor kinases: more than just kinases and not only for GPCRs

28 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4379943

An overview of enzymatic reagents for the removal of affinity tags

28 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4379943

Emerging paradigms of β-arrestin-dependent seven transmembrane receptor signaling

28 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4379943

Peptide surfactants for cell-free production of functional G protein-coupled receptors

28 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4379943

28 September 2017

Amphipols from A to Z.

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4379943

The variable detergent sensitivity of proteases that are utilized for recombinant protein affinity tag removal

28 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4379943

Thermostabilisation of an agonist-bound conformation of the human adenosine A(2A) receptor

28 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4379943

New advances in production and functional folding of G-protein-coupled receptors

28 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4379943

Maltose-neopentyl glycol (MNG) amphiphiles for solubilization, stabilization and crystallization of membrane proteins

28 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4379943

Production of membrane proteins without cells or detergents.

28 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4379943

NMR analyses of the interaction between CCR5 and its ligand using functional reconstitution of CCR5 in lipid bilayers.

28 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4379943

LCP-Tm: an assay to measure and understand stability of membrane proteins in a membrane environment

28 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4379943

Structure-based discovery of A2A adenosine receptor ligands

28 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4379943

A fluorescence anisotropy assay for the muscarinic M1 G-protein-coupled receptor

28 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4379943

Membrane protein expression in the eyes of transgenic flies

28 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4379943

Leukotriene BLT2 receptor monomers activate the G(i2) GTP-binding protein more efficiently than dimers

28 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4379943

Structural and functional characterization of the integral membrane protein VDAC-1 in lipid bilayer nanodiscs

28 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4379943

Effects of impurities on membrane-protein crystallization in different systems

28 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4379943

Engineering G protein-coupled receptors to facilitate their structure determination.

28 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4379943

Thermostabilization of the neurotensin receptor NTS1.

28 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4379943

Structure-based discovery of beta2-adrenergic receptor ligands

28 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4379943

A carboxy-terminal affinity tag for the purification and mass spectrometric characterization of integral membrane proteins

28 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4379943

Adenovirus mediated expression "in vivo" of the chemokine receptor CXCR1

28 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4379943

Efficient cell-free production of olfactory receptors: detergent optimization, structure, and ligand binding analyses

28 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4379943

Co-evolving stability and conformational homogeneity of the human adenosine A2a receptor

28 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4379943

GFP-based optimization scheme for the overexpression and purification of eukaryotic membrane proteins in Saccharomyces cerevisiae

28 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4379943

Cell-based assays in GPCR drug discovery

28 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4379943

Microscale fluorescent thermal stability assay for membrane proteins

28 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4379943

Analysis of a G protein-coupled receptor for neurotensin by liquid chromatography-electrospray ionization-mass spectrometry

28 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4379943

Stabilization of the human beta2-adrenergic receptor TM4-TM3-TM5 helix interface by mutagenesis of Glu122(3.41), a critical residue in GPCR structure

28 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4379943

Conformational thermostabilization of the beta1-adrenergic receptor in a detergent-resistant form

28 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4379943

Heterologous expression of the adenosine A1 receptor in transgenic mouse retina

28 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4379943

Transducin activation by nanoscale lipid bilayers containing one and two rhodopsins

28 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4379943

Detergents for the stabilization and crystallization of membrane proteins

28 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4379943

Use of dual affinity tags for expression and purification of functional peripheral cannabinoid receptor

28 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4379943

Fluorescence-detection size-exclusion chromatography for precrystallization screening of integral membrane proteins

28 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4379943

Enhancing functional production of G protein-coupled receptors in Pichia pastoris to levels required for structural studies via a single expression screen

28 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4379943

Crystallographic analysis of primary visual photochemistry

28 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4379943

Evaluation of detergents for the soluble expression of alpha-helical and beta-barrel-type integral membrane proteins by a preparative scale individual cell-free expression system

28 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4379943

Expression of functional G protein-coupled receptors in photoreceptors of transgenic Xenopus laevis

28 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4379943

Functional assays for screening GPCR targets

28 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4379943

Receptor systems: will mining the receptorome yield novel targets for pharmacotherapy?

28 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4379943

RONN: the bio-basis function neural network technique applied to the detection of natively disordered regions in proteins

28 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4379943

Structure of bovine rhodopsin in a trigonal crystal form.

28 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4379943

Differential dynamics in the G protein-coupled receptor rhodopsin revealed by solution NMR.

28 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4379943

Detergents Destabilize the Cubic Phase of Monoolein: Implications for Membrane Protein Crystallization

28 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4379943

A critical review of the methods for cleavage of fusion proteins with thrombin and factor Xa.

28 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4379943

Expression and purification of truncated, non-glycosylated turkey beta-adrenergic receptors for crystallization.

28 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4379943

Structure and function in rhodopsin: a tetracycline-inducible system in stable mammalian cell lines for high-level expression of opsin mutants

28 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4379943

Solution NMR spectroscopy of [alpha -15N]lysine-labeled rhodopsin: The single peak observed in both conventional and TROSY-type HSQC spectra is ascribed to Lys-339 in the carboxyl-terminal peptide sequence

28 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4379943

Membrane protein crystallization in lipidic mesophases: detergent effects

28 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4379943

X-Ray diffraction analysis of three-dimensional crystals of bovine rhodopsin obtained from mixed micelles.

28 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4379943

NMR spectroscopy in studies of light-induced structural changes in mammalian rhodopsin: applicability of solution (19)F NMR.

28 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4379943

Magic angle spinning NMR of the protonated retinylidene Schiff base nitrogen in rhodopsin: expression of 15N-lysine- and 13C-glycine-labeled opsin in a stable cell line

28 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4379943

Efficient generation of infectious recombinant baculoviruses by site-specific transposon-mediated insertion of foreign genes into a baculovirus genome propagated in Escherichia coli.

28 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4379943

Expression of a rat neurotensin receptor in Escherichia coli

28 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4379943

Expression of a synthetic bovine rhodopsin gene in monkey kidney cells

28 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4379943

Human beta 2-adrenergic receptors expressed in Escherichia coli membranes retain their pharmacological properties.

28 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4379943

28 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4379943

Platform for the rapid construction and evaluation of GPCRs for crystallography in Saccharomyces cerevisiae

28 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4379943

Crystal structure of a common GPCR-binding interface for G protein and arrestin

28 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4379943

N-terminal fusion tags for effective production of g-protein-coupled receptors in bacterial cell-free systems.

31 May 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4379943

Biased signaling pathways in β2-adrenergic receptor characterized by 19F-NMR.

31 May 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4379943

Engineering an ultra-thermostable β(1)-adrenoceptor

31 May 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4379943

Development of an Automated High Throughput LCP-FRAP Assay to Guide Membrane Protein Crystallization in Lipid Mesophases

31 May 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4379943

Cell-free synthesis of a functional G protein-coupled receptor complexed with nanometer scale bilayer discs

31 May 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4379943

Polymer-based cell-free expression of ligand-binding family B G-protein coupled receptors without detergents

31 May 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4379943

LCP-FRAP Assay for Pre-Screening Membrane Proteins for in Meso Crystallization

31 May 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4379943

Production and characterization of the recombinant human mu-opioid receptor from transgenic silkworms.

31 May 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4379943

Profiling of membrane protein variants in a baculovirus system by coupling cell-surface detection with small-scale parallel expression.

31 May 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4379943

Expression and purification of milligram levels of inactive G-protein coupled receptors in E. coli

31 May 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4379943

Functional reconstitution of purified metabotropic glutamate receptor expressed in the fly eye.

31 May 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4379943

Purification of proteins using polyhistidine affinity tags

31 May 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4379943

Purification of a rat neurotensin receptor expressed in Escherichia coli

31 May 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4379943

Amino and carboxyl terminal modifications to facilitate the production and purification of a G protein-coupled receptor

31 May 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4379943

A method for producing recombinant baculovirus expression vectors at high frequency.

31 May 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4379943

Reconstitutively active G protein-coupled receptors purified from baculovirus-infected insect cells

31 May 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4379943

Enhancement of membrane insertion and function in a type IIIb membrane protein following introduction of a cleavable signal peptide

31 May 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4379943

Expression of an olfactory receptor in Escherichia coli: purification, reconstitution, and ligand binding.

31 May 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4379943

Evolution of three human GPCRs for higher expression and stability.

31 May 2018

1 reference

https://api.crossref.org/works/10.3389%2FFPHAR.2015.00066

Optimization of membrane protein overexpression and purification using GFP fusions.

21 January 2018

1 reference

https://api.crossref.org/works/10.3389%2FFPHAR.2015.00066

A cleavable ligand column for the rapid isolation of large quantities of homogeneous and functional neurotensin receptor 1 variants from E. coli.

21 January 2018

1 reference

https://api.crossref.org/works/10.3389%2FFPHAR.2015.00066

NMR spectroscopy of phosphorylated wild-type rhodopsin: mobility of the phosphorylated C-terminus of rhodopsin in the dark and upon light activation

21 January 2018

1 reference

https://api.crossref.org/works/10.3389%2FFPHAR.2015.00066

A systematic approach to increase the efficiency of membrane protein production in cell-free expression systems.

21 January 2018

1 reference

https://api.crossref.org/works/10.3389%2FFPHAR.2015.00066

Prediction of disordered regions in proteins based on the meta approach

21 January 2018

1 reference

https://api.crossref.org/works/10.3389%2FFPHAR.2015.00066

Expression of G protein coupled receptors in a cell-free translational system using detergents and thioredoxin-fusion vectors

21 January 2018

1 reference

https://api.crossref.org/works/10.3389%2FFPHAR.2015.00066

Insertion of membrane proteins into discoidal membranes using a cell-free protein expression approach.

21 January 2018

1 reference

https://api.crossref.org/works/10.3389%2FFPHAR.2015.00066

Cell-free production of G protein-coupled receptors for functional and structural studies.

21 January 2018

1 reference

https://api.crossref.org/works/10.3389%2FFPHAR.2015.00066

Structural genomics on membrane proteins: comparison of more than 100 GPCRs in 3 expression systems.

21 January 2018

1 reference

https://api.crossref.org/works/10.3389%2FFPHAR.2015.00066

Optimization of protein production in mammalian cells with a coexpressed fluorescent marker

21 January 2018

1 reference

https://api.crossref.org/works/10.3389%2FFPHAR.2015.00066

Mammalian G-protein-coupled receptor expression in Escherichia coli: I. High-throughput large-scale production as inclusion bodies.

21 January 2018

1 reference

https://api.crossref.org/works/10.3389%2FFPHAR.2015.00066

Mammalian G protein-coupled receptor expression in Escherichia coli: II. Refolding and biophysical characterization of mouse cannabinoid receptor 1 and human parathyroid hormone receptor 1.

21 January 2018

1 reference

https://api.crossref.org/works/10.3389%2FFPHAR.2015.00066

The retinal conformation and its environment in rhodopsin in light of a new 2.2 A crystal structure

21 January 2018

1 reference

https://api.crossref.org/works/10.3389%2FFPHAR.2015.00066

Extensive proteolysis inhibits high-level production of eukaryal G protein-coupled receptors in the archaeon Haloferax volcanii

21 January 2018

1 reference

https://api.crossref.org/works/10.3389%2FFPHAR.2015.00066

Functional properties of cell-free expressed human endothelin A and endothelin B receptors in artificial membrane environments

21 January 2018

1 reference

https://api.crossref.org/works/10.3389%2FFPHAR.2015.00066

Employing Rhodobacter sphaeroides to functionally express and purify human G protein-coupled receptors.

21 January 2018

1 reference

https://api.crossref.org/works/10.3389%2FFPHAR.2015.00066

Improvements in G protein-coupled receptor purification yield light stable rhodopsin crystals

21 January 2018

1 reference

https://api.crossref.org/works/10.3389%2FFPHAR.2015.00066

Preparative scale expression of membrane proteins in Escherichia coli-based continuous exchange cell-free systems.

21 January 2018

1 reference

https://api.crossref.org/works/10.3389%2FFPHAR.2015.00066

Production of the stable human histamine H₁ receptor in Pichia pastoris for structural determination

21 January 2018

1 reference

https://api.crossref.org/works/10.3389%2FFPHAR.2015.00066

Efficient expression and immunoaffinity purification of human trace amine-associated receptor 5 from E. coli cell-free system

21 January 2018

1 reference

https://api.crossref.org/works/10.3389%2FFPHAR.2015.00066

Evaluation of cell-free expression system for the production of soluble and functional human GPCR N-formyl peptide receptors

21 January 2018

1 reference

https://api.crossref.org/works/10.3389%2FFPHAR.2015.00066

Development and crystallization of a minimal thermostabilised G protein-coupled receptor.

21 January 2018

1 reference

https://api.crossref.org/works/10.3389%2FFPHAR.2015.00066

Purification and characterization of the human adenosine A(2a) receptor functionally expressed in Escherichia coli

21 January 2018

1 reference

https://api.crossref.org/works/10.3389%2FFPHAR.2015.00066

An opsin mutant with increased thermal stability

21 January 2018

1 reference

https://api.crossref.org/works/10.3389%2FFPHAR.2015.00066

Comparison of functional non-glycosylated GPCRs expression in Pichia pastoris

21 January 2018

1 reference

https://api.crossref.org/works/10.3389%2FFPHAR.2015.00066

A Fluorescent Ligand-Binding Alternative Using Tag-lite® Technology

21 January 2018

1 reference

https://api.crossref.org/works/10.3389%2FFPHAR.2015.00066

Crystals of Native and Modified Bovine Rhodopsins and Their Heavy Atom Derivatives

21 January 2018

1 reference

https://api.crossref.org/works/10.3389%2FFPHAR.2015.00066

Advances in cell-free protein synthesis for the functional and structural analysis of membrane proteins

21 January 2018

1 reference

https://api.crossref.org/works/10.3389%2FFPHAR.2015.00066

Functional analysis of cell-free-produced human endothelin B receptor reveals transmembrane segment 1 as an essential area for ET-1 binding and homodimer formation

21 January 2018

1 reference

https://api.crossref.org/works/10.3389%2FFPHAR.2015.00066

Combined solid state and solution NMR studies of α,ɛ-15N labeled bovine rhodopsin

21 January 2018

1 reference

https://api.crossref.org/works/10.3389%2FFPHAR.2015.00066

Highly selective separation of rhodopsin from bovine rod outer segment membranes using combination of divalent cation and alkyl(thio)glucoside

21 January 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4379943

Expression of functional M2 muscarinic acetylcholine receptor in Escherichia coli

28 November 2018

1 reference

12 December 2020

Transferability of thermostabilizing mutations between β-adrenergic receptors

1 reference

12 December 2020

Functional expression of the human serotonin 5-HT1A receptor in Escherichia coli. Ligand binding properties and interaction with recombinant G protein alpha-subunits

1 reference

12 December 2020

Purification of the human G protein-coupled receptor adenosine A(2a)R in a stable and functional form expressed in Pichia pastoris

1 reference

12 December 2020

Purification of squid and octopus rhodopsin

1 reference

12 December 2020

Incorporation of in vitro synthesized GPCR into a tethered artificial lipid membrane system

1 reference

12 December 2020

Identifiers

DOI

10.3389/FPHAR.2015.00066

1 reference

https://www.ebi.ac.uk/europepmc/webservices/rest/search?query=EXT_ID:25873898%20AND%20SRC:MED&resulttype=core&format=json

9 June 2020

2 references

https://www.ebi.ac.uk/europepmc/webservices/rest/search?query=EXT_ID:25873898%20AND%20SRC:MED&resulttype=core&format=json

9 June 2020

PubMed publication ID

2 references