(Q47281660)

13 January 2018

review article

1 reference

title

Am I ready for CRISPR? A user's guide to genetic screens (English)

1 reference

13 January 2018

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1

John G Doench

1 reference

13 January 2018

publication date

4 December 2017

1 reference

13 January 2018

published in

Nature Reviews Genetics

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13 January 2018

https://scigraph.springernature.com/pub.10.1038/nrg.2017.97

exact match

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cites work

A global reference for human genetic variation

1 reference

https://api.crossref.org/works/10.1038%2FNRG.2017.97

Disciplined approach to drug discovery and early development

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNRG.2017.97

A large-scale RNAi screen in human cells identifies new components of the p53 pathway

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNRG.2017.97

A genetic screen for candidate tumor suppressors identifies REST

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNRG.2017.97

Genome-scale CRISPR-Cas9 knockout screening in human cells

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNRG.2017.97

Genetic screens in human cells using the CRISPR-Cas9 system

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNRG.2017.97

Genome-wide recessive genetic screening in mammalian cells with a lentiviral CRISPR-guide RNA library

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNRG.2017.97

Highly parallel identification of essential genes in cancer cells

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNRG.2017.97

Viral Packaging and Cell Culture for CRISPR-Based Screens

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNRG.2017.97

Genetic screens and functional genomics using CRISPR/Cas9 technology

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNRG.2017.97

Single Guide RNA Library Design and Construction

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNRG.2017.97

Validation of Synthetic CRISPR Reagents as a Tool for Arrayed Functional Genomic Screening

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNRG.2017.97

Orthogonal Cas9 proteins for RNA-guided gene regulation and editing

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNRG.2017.97

Efficient genome engineering in human pluripotent stem cells using Cas9 from Neisseria meningitidis

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNRG.2017.97

Discovery and Functional Characterization of Diverse Class 2 CRISPR-Cas Systems

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNRG.2017.97

In vivo genome editing using Staphylococcus aureus Cas9

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNRG.2017.97

Cpf1 is a single RNA-guided endonuclease of a class 2 CRISPR-Cas system

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNRG.2017.97

Diverse evolutionary roots and mechanistic variations of the CRISPR-Cas systems

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNRG.2017.97

Rational design of highly active sgRNAs for CRISPR-Cas9-mediated gene inactivation.

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNRG.2017.97

Systematic investigation of genetic vulnerabilities across cancer cell lines reveals lineage-specific dependencies in ovarian cancer

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNRG.2017.97

Gene Essentiality Profiling Reveals Gene Networks and Synthetic Lethal Interactions with Oncogenic Ras.

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNRG.2017.97

A CRISPR Dropout Screen Identifies Genetic Vulnerabilities and Therapeutic Targets in Acute Myeloid Leukemia.

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNRG.2017.97

MTAP deletion confers enhanced dependency on the PRMT5 arginine methyltransferase in cancer cells

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNRG.2017.97

Defining a Cancer Dependency Map.

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNRG.2017.97

Project DRIVE: A Compendium of Cancer Dependencies and Synthetic Lethal Relationships Uncovered by Large-Scale, Deep RNAi Screening

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNRG.2017.97

High-Resolution CRISPR Screens Reveal Fitness Genes and Genotype-Specific Cancer Liabilities.

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNRG.2017.97

A Genome-wide CRISPR Death Screen Identifies Genes Essential for Oxidative Phosphorylation.

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNRG.2017.97

Synergistic interactions with PI3K inhibition that induce apoptosis.

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNRG.2017.97

Alternative to the soft-agar assay that permits high-throughput drug and genetic screens for cellular transformation

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNRG.2017.97

Hypoxia as a therapy for mitochondrial disease.

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNRG.2017.97

ATXN1L, CIC, and ETS Transcription Factors Modulate Sensitivity to MAPK Pathway Inhibition.

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNRG.2017.97

KEAP1 loss modulates sensitivity to kinase targeted therapy in lung cancer

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNRG.2017.97

A Vulnerability of a Subset of Colon Cancers with Potential Clinical Utility.

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNRG.2017.97

A melanocyte lineage program confers resistance to MAP kinase pathway inhibition

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNRG.2017.97

Discovery of a proteinaceous cellular receptor for a norovirus

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNRG.2017.97

A CRISPR-Based Screen Identifies Genes Essential for West-Nile-Virus-Induced Cell Death

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNRG.2017.97

CRISPR/Cas9 Screens Reveal Requirements for Host Cell Sulfation and Fucosylation in Bacterial Type III Secretion System-Mediated Cytotoxicity

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNRG.2017.97

A CRISPR screen defines a signal peptide processing pathway required by flaviviruses

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNRG.2017.97

Genetic dissection of Flaviviridae host factors through genome-scale CRISPR screens

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNRG.2017.97

Parallel shRNA and CRISPR-Cas9 screens enable antiviral drug target identification

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNRG.2017.97

CRISPR/Cas9 Screens Reveal Epstein-Barr Virus-Transformed B Cell Host Dependency Factors

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNRG.2017.97

A Genome-wide CRISPR Screen in Primary Immune Cells to Dissect Regulatory Networks

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNRG.2017.97

Genetic wiring maps of single-cell protein states reveal an off-switch for GPCR signalling.

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNRG.2017.97

A genome-wide CRISPR screen identifies a restricted set of HIV host dependency factors.

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNRG.2017.97

Versatile in vivo regulation of tumor phenotypes by dCas9-mediated transcriptional perturbation.

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNRG.2017.97

RNAi screens in mice identify physiological regulators of oncogenic growth

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNRG.2017.97

Direct in vivo RNAi screen unveils myosin IIa as a tumor suppressor of squamous cell carcinomas

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNRG.2017.97

Generation of mouse models of myeloid malignancy with combinatorial genetic lesions using CRISPR-Cas9 genome editing.

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNRG.2017.97

In vivo CRISPR screening identifies Ptpn2 as a cancer immunotherapy target.

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNRG.2017.97

Efficient tumour formation by single human melanoma cells

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNRG.2017.97

Inducible RNAi in vivo reveals that the transcription factor BATF is required to initiate but not maintain CD8+ T-cell effector differentiation

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNRG.2017.97

Studying clonal dynamics in response to cancer therapy using high-complexity barcoding.

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNRG.2017.97

Perturb-Seq: Dissecting Molecular Circuits with Scalable Single-Cell RNA Profiling of Pooled Genetic Screens

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNRG.2017.97

A Multiplexed Single-Cell CRISPR Screening Platform Enables Systematic Dissection of the Unfolded Protein Response

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNRG.2017.97

Pooled CRISPR screening with single-cell transcriptome readout

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNRG.2017.97

Dissecting Immune Circuits by Linking CRISPR-Pooled Screens with Single-Cell RNA-Seq

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNRG.2017.97

Multiplex genome engineering using CRISPR/Cas systems

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNRG.2017.97

RNA-guided human genome engineering via Cas9

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNRG.2017.97

RNA-programmed genome editing in human cells

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNRG.2017.97

Repurposing CRISPR as an RNA-guided platform for sequence-specific control of gene expression

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNRG.2017.97

CAS9 transcriptional activators for target specificity screening and paired nickases for cooperative genome engineering

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNRG.2017.97

CRISPR-mediated modular RNA-guided regulation of transcription in eukaryotes

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNRG.2017.97

Genome-Scale CRISPR-Mediated Control of Gene Repression and Activation

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNRG.2017.97

Genome-scale transcriptional activation by an engineered CRISPR-Cas9 complex

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNRG.2017.97

High-throughput mapping of regulatory DNA.

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNRG.2017.97

Repurposing the CRISPR-Cas9 system for targeted DNA methylation

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNRG.2017.97

CRISPR-dCas9 mediated TET1 targeting for selective DNA demethylation at BRCA1 promoter.

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNRG.2017.97

Epigenome editing by a CRISPR-Cas9-based acetyltransferase activates genes from promoters and enhancers.

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNRG.2017.97

Functional annotation of native enhancers with a Cas9-histone demethylase fusion.

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNRG.2017.97

Locus-specific histone deacetylation using a synthetic CRISPR-Cas9-based HDAC

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNRG.2017.97

Genome-wide specificity of DNA binding, gene regulation, and chromatin remodeling by TALE- and CRISPR/Cas9-based transcriptional activators

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNRG.2017.97

Highly specific epigenome editing by CRISPR-Cas9 repressors for silencing of distal regulatory elements

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNRG.2017.97

Programmable editing of a target base in genomic DNA without double-stranded DNA cleavage

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNRG.2017.97

Directed evolution using dCas9-targeted somatic hypermutation in mammalian cells.

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNRG.2017.97

Targeted AID-mediated mutagenesis (TAM) enables efficient genomic diversification in mammalian cells

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNRG.2017.97

Next-generation libraries for robust RNA interference-based genome-wide screens.

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNRG.2017.97

Systematic comparison of CRISPR/Cas9 and RNAi screens for essential genes

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNRG.2017.97

CRISPR knockout screening outperforms shRNA and CRISPRi in identifying essential genes.

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNRG.2017.97

Experimental validation of the importance of seed complement frequency to siRNA specificity

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNRG.2017.97

Morphological Profiles of RNAi-Induced Gene Knockdown Are Highly Reproducible but Dominated by Seed Effects

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNRG.2017.97

High-throughput Phenotyping of Lung Cancer Somatic Mutations

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNRG.2017.97

Prospective functional classification of all possible missense variants in PPARG.

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNRG.2017.97

Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9.

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNRG.2017.97

Identification and characterization of essential genes in the human genome.

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNRG.2017.97

Nucleosomes impede Cas9 access to DNA in vivo and in vitro

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNRG.2017.97

Flexible CRISPR library construction using parallel oligonucleotide retrieval.

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNRG.2017.97

Guide Picker is a comprehensive design tool for visualizing and selecting guides for CRISPR experiments.

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNRG.2017.97

Discovery of cancer drug targets by CRISPR-Cas9 screening of protein domains

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNRG.2017.97

CRISPR Screens Provide a Comprehensive Assessment of Cancer Vulnerabilities but Generate False-Positive Hits for Highly Amplified Genomic Regions.

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNRG.2017.97

APPRIS: annotation of principal and alternative splice isoforms

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNRG.2017.97

Sequence determinants of improved CRISPR sgRNA design

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNRG.2017.97

Evaluation of off-target and on-target scoring algorithms and integration into the guide RNA selection tool CRISPOR.

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNRG.2017.97

DNA Repair Profiling Reveals Nonrandom Outcomes at Cas9-Mediated Breaks.

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNRG.2017.97

Microhomology-based choice of Cas9 nuclease target sites.

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNRG.2017.97

Defining and improving the genome-wide specificities of CRISPR-Cas9 nucleases

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNRG.2017.97

Optimizing sgRNA position markedly improves the efficiency of CRISPR/dCas9-mediated transcriptional repression

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNRG.2017.97

Update of the FANTOM web resource: high resolution transcriptome of diverse cell types in mammals

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNRG.2017.97

Resources for the design of CRISPR gene editing experiments

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNRG.2017.97

E-CRISP: fast CRISPR target site identification.

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNRG.2017.97

GUIDES: sgRNA design for loss-of-function screens.

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNRG.2017.97

Cas-OFFinder: a fast and versatile algorithm that searches for potential off-target sites of Cas9 RNA-guided endonucleases

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNRG.2017.97

Systematic mapping of functional enhancer-promoter connections with CRISPR interference

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNRG.2017.97

Variant-aware saturating mutagenesis using multiple Cas9 nucleases identifies regulatory elements at trait-associated loci.

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNRG.2017.97

BCL11A enhancer dissection by Cas9-mediated in situ saturating mutagenesis

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNRG.2017.97

CRISPRi-based genome-scale identification of functional long noncoding RNA loci in human cells

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNRG.2017.97

caRpools: an R package for exploratory data analysis and documentation of pooled CRISPR/Cas9 screens.

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNRG.2017.97

Evaluation and Design of Genome-Wide CRISPR/SpCas9 Knockout Screens.

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNRG.2017.97

Measuring error rates in genomic perturbation screens: gold standards for human functional genomics

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNRG.2017.97

Creation of Novel Protein Variants with CRISPR/Cas9-Mediated Mutagenesis: Turning a Screening By-Product into a Discovery Tool

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNRG.2017.97

Rapid generation of drug-resistance alleles at endogenous loci using CRISPR-Cas9 indel mutagenesis.

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNRG.2017.97

Genomic Copy Number Dictates a Gene-Independent Cell Response to CRISPR/Cas9 Targeting

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNRG.2017.97

Complementary information derived from CRISPR Cas9 mediated gene deletion and suppression

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNRG.2017.97

Easy quantitative assessment of genome editing by sequence trace decomposition

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNRG.2017.97

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNRG.2017.97

Derivation and differentiation of haploid human embryonic stem cells

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNRG.2017.97

GenomeCRISPR - a database for high-throughput CRISPR/Cas9 screens.

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNRG.2017.97

Synergistic drug combinations for cancer identified in a CRISPR screen for pairwise genetic interactions

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNRG.2017.97

Multiplexed barcoded CRISPR-Cas9 screening enabled by CombiGEM.

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNRG.2017.97

Atomic segregation: Activity at the surface.

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNRG.2017.97

A systematic mammalian genetic interaction map reveals pathways underlying ricin susceptibility

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNRG.2017.97

A global genetic interaction network maps a wiring diagram of cellular function

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNRG.2017.97

Exploring genetic suppression interactions on a global scale

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNRG.2017.97

Applications of CRISPR genome editing technology in drug target identification and validation

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNRG.2017.97

Target identification and mechanism of action in chemical biology and drug discovery

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNRG.2017.97

Comparison of Cas9 activators in multiple species

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNRG.2017.97

Editing DNA Methylation in the Mammalian Genome

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNRG.2017.97

Strategies for precision modulation of gene expression by epigenome editing: an overview

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNRG.2017.97

GUIDE-seq enables genome-wide profiling of off-target cleavage by CRISPR-Cas nucleases

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNRG.2017.97

Compact and highly active next-generation libraries for CRISPR-mediated gene repression and activation.

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNRG.2017.97

MAGeCK enables robust identification of essential genes from genome-scale CRISPR/Cas9 knockout screens

31 March 2020

1 reference

PubMed

https://pubmed.ncbi.nlm.nih.gov/29199283

12 December 2020

inferred from PubMed ID database lookup

Combinatorial CRISPR-Cas9 screens for de novo mapping of genetic interactions.

1 reference

PubMed

https://pubmed.ncbi.nlm.nih.gov/29199283

12 December 2020

inferred from PubMed ID database lookup

Evaluation of RNAi and CRISPR technologies by large-scale gene expression profiling in the Connectivity Map.

1 reference

PubMed

https://pubmed.ncbi.nlm.nih.gov/29199283

12 December 2020

inferred from PubMed ID database lookup

Identifiers

DOI

10.1038/NRG.2017.97

1 reference

13 January 2018

release_gphaqdztnzcidmrnvrblbqkvmi

Fatcat ID

1 reference

Fatcat

https://api.fatcat.wiki/v0/release/gphaqdztnzcidmrnvrblbqkvmi

24 November 2022

mapped directly with Wikidata item

1 reference

13 January 2018